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. 1983 Sep 24;11(18):6453–6464. doi: 10.1093/nar/11.18.6453

Differential inhibition of cellular RNAs by photosensitized trioxalen.

B Shames, S Kalma, Y M Heimer, J Tal
PMCID: PMC326385  PMID: 6194509

Abstract

Treatment of mouse cell cultures with 5 X 10(-6)M trioxalen (4,5',8-trimethylpsoralen, or TMP) followed by long wavelength ("near") UV-irradiation (NUV) resulted in immediate cessation of most of the cellular transcriptional activity. The effective inhibitory TMP concentrations for ribosomal RNA were lower than those affecting poly RNA containing RNA. Low molecular RNA species were only partially inhibited. Side effects, commonly caused by other drugs, were not detected. These properties, and the simplicity of their use, make psoralens attractive inhibitors of RNA biosynthesis.

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Selected References

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  1. Cole R. S. Light-induced cross-linking of DNA in the presence of a furocoumarin (psoralen). Studies with phage lambda, Escherichia coli, and mouse leukemia cells. Biochim Biophys Acta. 1970 Sep 17;217(1):30–39. doi: 10.1016/0005-2787(70)90119-x. [DOI] [PubMed] [Google Scholar]
  2. Frederiksen S., Hearst J. E. Binding of 4'-aminomethyl 4,5',8-trimethyl psoralen to DNA, RNA and protein in HeLa cells and Drosophila cells. Biochim Biophys Acta. 1979 Jul 26;563(2):343–355. doi: 10.1016/0005-2787(79)90053-4. [DOI] [PubMed] [Google Scholar]
  3. Goldstein E. S., Penman S. Regulation of protein synthesis in mammalian cells. V. Further studies on the effect of actinomycin D on translation control in HeLa cells. J Mol Biol. 1973 Oct 25;80(2):243–254. doi: 10.1016/0022-2836(73)90170-8. [DOI] [PubMed] [Google Scholar]
  4. Hamelin R., Larsen C. J., Tavitian A. Effects of actinomycin D, toyocamycin and cycloheximide on the synthesis of low-molecular-weight nuclear RNAs in HeLa cells. Eur J Biochem. 1973 Jun;35(2):350–356. doi: 10.1111/j.1432-1033.1973.tb02846.x. [DOI] [PubMed] [Google Scholar]
  5. Harada F., Dahlberg J. E. Specific cleavage of tRNA by nuclease S1. Nucleic Acids Res. 1975 Jun;2(6):865–871. doi: 10.1093/nar/2.6.865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Heimer Y. M., Ben-Hur E., Riklis E. Photosensitized inhibition of nitrate reductase induction by 4,5',8-trimethylpsoralen and near ultraviolet light. Biochim Biophys Acta. 1978 Jul 24;519(2):499–506. doi: 10.1016/0005-2787(78)90102-8. [DOI] [PubMed] [Google Scholar]
  7. Heimer Y. M., Ben-Hur E., Riklis E. Psoralen and near ultraviolet light; a probe for study of control of protein synthesis. Nature. 1977 Jul 14;268(5616):170–171. doi: 10.1038/268170a0. [DOI] [PubMed] [Google Scholar]
  8. Hyde J. E., Hearst J. E. Binding of psoralen derivatives to DNA and chromatin: influence of the ionic environment on dark binding and photoreactivity. Biochemistry. 1978 Apr 4;17(7):1251–1257. doi: 10.1021/bi00600a019. [DOI] [PubMed] [Google Scholar]
  9. Hyman R. W., Davidson N. Kinetics of the in vitro inhibition of transcription by actinomycin. J Mol Biol. 1970 Jun 14;50(2):421–438. doi: 10.1016/0022-2836(70)90202-0. [DOI] [PubMed] [Google Scholar]
  10. Isaacs S. T., Shen C. K., Hearst J. E., Rapoport H. Synthesis and characterization of new psoralen derivatives with superior photoreactivity with DNA and RNA. Biochemistry. 1977 Mar 22;16(6):1058–1064. doi: 10.1021/bi00625a005. [DOI] [PubMed] [Google Scholar]
  11. LERNER A. B., DENTON C. R., FITZPATRICK T. B. Clinical and experimental studies with 8-methoxypsoralen in vitiligo. J Invest Dermatol. 1953 Apr;20(4):299–314. doi: 10.1038/jid.1953.36. [DOI] [PubMed] [Google Scholar]
  12. Maitra U., Nakata Y., Hurwitz J. The role of deoxyribonucleic acid in ribonucleic acid synthesis. XIV. A study of the initiation of ribonucleic acid synthesis. J Biol Chem. 1967 Nov 10;242(21):4908–4918. [PubMed] [Google Scholar]
  13. Musajo L., Rodighiero G. Studies on the photo-C4-cyclo-addition reactions between skin-photosensitizing furocoumarins and nucleic acids. Photochem Photobiol. 1970 Jan;11(1):27–35. doi: 10.1111/j.1751-1097.1970.tb05714.x. [DOI] [PubMed] [Google Scholar]
  14. Nakashima K., LaFiandra A. J., Shatkin A. J. Differential dependence of reovirus-associated enzyme activities on genome RNA as determined by psoralen photosensitivity. J Biol Chem. 1979 Aug 25;254(16):8007–8014. [PubMed] [Google Scholar]
  15. Ou C. N., Tsai C. H., Tapley K. J., Jr, Song P. S. Photobinding of 8-methoxypsoralen and 5,7-dimethoxycoumarin to DNA and its effect on template activity. Biochemistry. 1978 Mar 21;17(6):1047–1053. doi: 10.1021/bi00599a017. [DOI] [PubMed] [Google Scholar]
  16. Parrish J. A., Fitzpatrick T. B., Tanenbaum L., Pathak M. A. Photochemotherapy of psoriasis with oral methoxsalen and longwave ultraviolet light. N Engl J Med. 1974 Dec 5;291(23):1207–1211. doi: 10.1056/NEJM197412052912301. [DOI] [PubMed] [Google Scholar]
  17. Scholtissek C. Unphysiological breakdown of fast-labelled RNA by actinomycin D in primary chick fibroblasts. Eur J Biochem. 1972 Jun 23;28(1):70–73. doi: 10.1111/j.1432-1033.1972.tb01884.x. [DOI] [PubMed] [Google Scholar]
  18. Singer R. H., Penman S. Stability of HeLa cell mRNA in actinomycin. Nature. 1972 Nov 10;240(5376):100–102. doi: 10.1038/240100a0. [DOI] [PubMed] [Google Scholar]
  19. Song P. S., Tapley K. J., Jr Photochemistry and photobiology of psoralens. Photochem Photobiol. 1979 Jun;29(6):1177–1197. doi: 10.1111/j.1751-1097.1979.tb07838.x. [DOI] [PubMed] [Google Scholar]
  20. Stern R., Friedman R. M. Double-stranded RNA synthesized in animal cells in the presence of actinomycin D. Nature. 1970 May 16;226(5246):612–616. doi: 10.1038/226612a0. [DOI] [PubMed] [Google Scholar]
  21. Stern R., Friedman R. M. Ribonucleic acid synthesis in animal cells in the presence of actinomycin. Biochemistry. 1971 Sep 28;10(20):3635–3645. doi: 10.1021/bi00796a001. [DOI] [PubMed] [Google Scholar]
  22. Swan D., Aviv H., Leder P. Purification and properties of biologically active messenger RNA for a myeloma light chain. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1967–1971. doi: 10.1073/pnas.69.7.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Swanstrom R., Hallick L. M., Jackson J., Hearst J. E., Bishop J. M. Interaction of psoralen derivatives with the RNA genome of Rous sarcoma virus. Virology. 1981 Sep;113(2):613–622. doi: 10.1016/0042-6822(81)90189-6. [DOI] [PubMed] [Google Scholar]
  24. Tal J. The cleavage of transfer RNA by a single strang specific endonuclease from Neurospora crassa. Nucleic Acids Res. 1975 Jul;2(7):1073–1082. doi: 10.1093/nar/2.7.1073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wolff K., Gschnait F., Hönigsmann H., Konrad K., Parrish J. A., Fitzpatrick T. B. Phototesting and dosimetry for photochemotherapy. Br J Dermatol. 1977 Jan;96(1):1–10. doi: 10.1111/j.1365-2133.1977.tb05177.x. [DOI] [PubMed] [Google Scholar]

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