Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1983 Oct 25;11(20):7205–7214. doi: 10.1093/nar/11.20.7205

The sequences of rabbit kappa light chains of b4 and b5 allotypes differ more in their constant regions than in their 3' untranslated regions.

K E Bernstein, R M Skurla Jr, R G Mage
PMCID: PMC326455  PMID: 6314281

Abstract

We report the sequence of a cDNA clone encoding the entire variable and constant regions of a rabbit kappa light chain of b5 allotype. The deduced amino acid sequence of the variable region (positions 1-95) is 86% homologous to that of a b4 light chain protein [BS-1) (1) but the b4 and b5 constant regions are only 74% homologous. Comparison of this DNA sequence to that of a cDNA clone encoding a b4 constant region shows that the kappa allotypes b4 and b5 have diverged significantly more in their coding region than in the 3' untranslated regions (86% vs 96% nucleotide sequence homologies). This implies either a function for the 3' untranslated region with evolutionary pressures to conserve or an accelerated divergence of the coding regions.

Full text

PDF
7208

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alt F. W., Baltimore D. Joining of immunoglobulin heavy chain gene segments: implications from a chromosome with evidence of three D-JH fusions. Proc Natl Acad Sci U S A. 1982 Jul;79(13):4118–4122. doi: 10.1073/pnas.79.13.4118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Auffray C., Sikorav J. L., Ollo R., Rougeon F. Correlation between D region structure and antigen-binding specificity: evidences from the comparison of closely related immunoglobulin VH sequences. Ann Immunol (Paris) 1981 Jul-Aug;132D(1):77–88. [PubMed] [Google Scholar]
  3. Ayadi H., Dutka S., Paroutaud P., Strosberg A. D. Partial amino acid sequence of a rabbit immunoglobulin light chain of allotype b5. Biochemistry. 1983 Feb 15;22(4):993–998. doi: 10.1021/bi00273a044. [DOI] [PubMed] [Google Scholar]
  4. Ayadi H., Paroutaud P., Benammar A., Cazenave P. A., Strosberg A. D. Structural studies of a wild rabbit immunoglobulin light chain constant region. Mol Immunol. 1983 Feb;20(2):223–227. doi: 10.1016/0161-5890(83)90134-7. [DOI] [PubMed] [Google Scholar]
  5. Benammar A., Cazenave P. A. A second rabbit kappa isotype. J Exp Med. 1982 Aug 1;156(2):585–595. doi: 10.1084/jem.156.2.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bentley D. L., Rabbitts T. H. Human immunoglobulin variable region genes--DNA sequences of two V kappa genes and a pseudogene. Nature. 1980 Dec 25;288(5792):730–733. doi: 10.1038/288730a0. [DOI] [PubMed] [Google Scholar]
  7. Bernstein K. E., Pavirani A., Alexander C., Jacobsen F., Fitzmaurice L., Mage R. Use of Trypanosoma equiperdum infected rabbits as a source of splenic mRNA; construction of cDNA clones and identification of a rabbit mu heavy chain clone. Mol Immunol. 1983 Jan;20(1):89–99. doi: 10.1016/0161-5890(83)90108-6. [DOI] [PubMed] [Google Scholar]
  8. Braun D. G., Huser H., Knecht R., Wright J. K. Amino acid sequences of antibody light chain variable regions of pedigreed rabbits: kappa light chain K49-501 (allotype b4 anti-streptococcal group A-variant polysaccharide antibody). Hoppe Seylers Z Physiol Chem. 1979 May;360(5):663–667. doi: 10.1515/bchm2.1979.360.1.663. [DOI] [PubMed] [Google Scholar]
  9. Chersi A., Alexander C. B., Mage R. Partial primary structure of the immunoglobulin light chain constant region of a single rabbit of b5 allotype. Mol Immunol. 1980 Dec;17(12):1515–1523. doi: 10.1016/0161-5890(80)90177-7. [DOI] [PubMed] [Google Scholar]
  10. Evans G. A., Margulies D. H., Camerini-Otero R. D., Ozato K., Seidman J. G. Structure and expression of a mouse major histocompatibility antigen gene, H-2Ld. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1994–1998. doi: 10.1073/pnas.79.6.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Farnsworth V., Goodfliesh R., Rodkey S., Hood L. Immunoglobulin allotypes of rabbit kappa chains: polymorphism of a control mechanism regulating closely linked duplicated genes? Proc Natl Acad Sci U S A. 1976 Apr;73(4):1293–1296. doi: 10.1073/pnas.73.4.1293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hardison R. C., Butler E. T., 3rd, Lacy E., Maniatis T., Rosenthal N., Efstratiadis A. The structure and transcription of four linked rabbit beta-like globin genes. Cell. 1979 Dec;18(4):1285–1297. doi: 10.1016/0092-8674(79)90239-3. [DOI] [PubMed] [Google Scholar]
  13. Heidmann O., Auffray C., Cazenave P. A., Rougeon F. Nucleotide sequence of constant and 3' untranslated regions of a kappa immunoglobulin light chain mRNA of a homozygous b4 rabbit. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5802–5806. doi: 10.1073/pnas.78.9.5802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Heidmann O., Rougeon F. Multiple sequences related to a constant-region kappa light chain gene in the rabbit genome. Cell. 1982 Mar;28(3):507–513. doi: 10.1016/0092-8674(82)90205-7. [DOI] [PubMed] [Google Scholar]
  15. Heidmann O., Rougeon F. Multiplicity of constant kappa light chain genes in the rabbit genome: a b4b4 homozygous rabbit contains a kappa-bas gene. EMBO J. 1983;2(3):437–441. doi: 10.1002/j.1460-2075.1983.tb01441.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hieter P. A., Maizel J. V., Jr, Leder P. Evolution of human immunoglobulin kappa J region genes. J Biol Chem. 1982 Feb 10;257(3):1516–1522. [PubMed] [Google Scholar]
  17. Hieter P. A., Max E. E., Seidman J. G., Maizel J. V., Jr, Leder P. Cloned human and mouse kappa immunoglobulin constant and J region genes conserve homology in functional segments. Cell. 1980 Nov;22(1 Pt 1):197–207. doi: 10.1016/0092-8674(80)90168-3. [DOI] [PubMed] [Google Scholar]
  18. Jaton J. C. The V-region sequence of the H chain from a third rabbit anti-pneumococcal antibody. Biochem J. 1976 Aug 1;157(2):449–459. doi: 10.1042/bj1570449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kindt T. J. Rabbit immunoglobulin allotypes: structure, immunology, and genetics. Adv Immunol. 1975;21:35–86. doi: 10.1016/s0065-2776(08)60218-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kurosawa Y., Tonegawa S. Organization, structure, and assembly of immunoglobulin heavy chain diversity DNA segments. J Exp Med. 1982 Jan 1;155(1):201–218. doi: 10.1084/jem.155.1.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Larhammar D., Gustafsson K., Claesson L., Bill P., Wiman K., Schenning L., Sundelin J., Widmark E., Peterson P. A., Rask L. Alpha chain of HLA-DR transplantation antigens is a member of the same protein superfamily as the immunoglobulins. Cell. 1982 Aug;30(1):153–161. doi: 10.1016/0092-8674(82)90021-6. [DOI] [PubMed] [Google Scholar]
  22. Mage R. The phenotypic expression of rabbit immunoglobulins: A model of complex regulated gene expression and cellular differentiation. Contemp Top Mol Immunol. 1981;8:89–112. doi: 10.1007/978-1-4684-3917-5_3. [DOI] [PubMed] [Google Scholar]
  23. Max E. E., Seidman J. G., Leder P. Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3450–3454. doi: 10.1073/pnas.76.7.3450. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  25. McCartney-Francis N., Mandy W. J. Control of latent allotype expression by rabbit splenocytes. J Immunol. 1981 Jul;127(1):352–357. [PubMed] [Google Scholar]
  26. Moulton J. E., Coleman J. L., Gee M. K. Pathogenesis of Trypanosoma equiperdum in rabbits. Am J Vet Res. 1975 Apr;36(4 PT1):357–366. [PubMed] [Google Scholar]
  27. Ollo R., Rougeon F. Gene conversion and polymorphism: generation of mouse immunoglobulin gamma 2a chain alleles by differential gene conversion by gamma 2b chain gene. Cell. 1983 Feb;32(2):515–523. doi: 10.1016/0092-8674(83)90471-3. [DOI] [PubMed] [Google Scholar]
  28. Pavirani A., Mage R., Jacobsen F. W., Reddy E. P., Bernstein K., Fitzmaurice L. Construction of a partial rabbit spleen cDNA library and identification of immunoglobulin clones. Eur J Immunol. 1982 Oct;12(10):854–860. doi: 10.1002/eji.1830121011. [DOI] [PubMed] [Google Scholar]
  29. Pavirani A., McCartney-Francis N., Jacobsen F., Mage R. G., Reddy E. P., Fitzmaurice L. C. Analyses of the splenic mRNA expressed by rabbits of different immunoglobulin kappa-light chain allotypes: conserved sequences in the 3' untranslated region and allotype-specific probes. J Immunol. 1983 Aug;131(2):1000–1006. [PubMed] [Google Scholar]
  30. Pease L. R., Schulze D. H., Pfaffenbach G. M., Nathenson S. G. Spontaneous H-2 mutants provide evidence that a copy mechanism analogous to gene conversion generates polymorphism in the major histocompatibility complex. Proc Natl Acad Sci U S A. 1983 Jan;80(1):242–246. doi: 10.1073/pnas.80.1.242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Rose L. M., Goldman M., Lambert P. H. Simultaneous induction of an idiotype, corresponding anti-idiotypic antibodies, and immune complexes during African trypanosomiasis in mice. J Immunol. 1982 Jan;128(1):79–85. [PubMed] [Google Scholar]
  32. Sakano H., Kurosawa Y., Weigert M., Tonegawa S. Identification and nucleotide sequence of a diversity DNA segment (D) of immunoglobulin heavy-chain genes. Nature. 1981 Apr 16;290(5807):562–565. doi: 10.1038/290562a0. [DOI] [PubMed] [Google Scholar]
  33. Schreier P. H., Bothwell A. L., Mueller-Hill B., Baltimore D. Multiple differences between the nucleic acid sequences of the IgG2aa and IgG2ab alleles of the mouse. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4495–4499. doi: 10.1073/pnas.78.7.4495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Scott C. L., Potter M. Diversity of immunoglobulin structural gene loci. The special case of the mouse lambda locus. Surv Immunol Res. 1983;2(1):43–51. [PubMed] [Google Scholar]
  35. Sheppard H. W., Gutman G. A. Allelic forms of rat kappa chain genes: evidence for strong selection at the level of nucleotide sequence. Proc Natl Acad Sci U S A. 1981 Nov;78(11):7064–7068. doi: 10.1073/pnas.78.11.7064. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Sogn J. A., Kindt T. J. A genetic polymorphism in the constant region of rabbit b4 kappa chains. J Exp Med. 1976 Jun 1;143(6):1475–1482. doi: 10.1084/jem.143.6.1475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Weiss E. H., Mellor A., Golden L., Fahrner K., Simpson E., Hurst J., Flavell R. A. The structure of a mutant H-2 gene suggests that the generation of polymorphism in H-2 genes may occur by gene conversion-like events. Nature. 1983 Feb 24;301(5902):671–674. doi: 10.1038/301671a0. [DOI] [PubMed] [Google Scholar]
  38. van Ooyen A., van den Berg J., Mantei N., Weissmann C. Comparison of total sequence of a cloned rabbit beta-globin gene and its flanking regions with a homologous mouse sequence. Science. 1979 Oct 19;206(4416):337–344. doi: 10.1126/science.482942. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES