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. 1983 Nov 25;11(22):8073–8085. doi: 10.1093/nar/11.22.8073

The major coat protein gene of the filamentous Pseudomonas aeruginosa phage Pf3: absence of an N-terminal leader signal sequence.

R G Luiten, J G Schoenmakers, R N Konings
PMCID: PMC326561  PMID: 6316283

Abstract

From in vitro protein synthesis studies and nucleotide sequence analysis it has been deduced that, unlike the major coat proteins of the hitherto studied filamentous bacterial viruses Ff (M13, fd and f1), IKe and Pf1, the major coat protein of the filamentous Pseudomonas aeruginosa virus Pf3 is not synthesized as a precursor containing a leader signal polypeptide at its N-terminal end. From the elucidated nucleotide sequence of the Pf3 major coat protein gene it follows that the coat protein is 44 amino acid residues long (mol.wt. 6425). No sequence homology was observed with the major coat protein genes of either the Ff group or IKe but, similar to these phages, 3' ward of the Pf3 coat protein gene a DNA sequence is located which has many characteristics in common with rho-independent transcription termination signals.

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Selected References

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  1. Beck E., Sommer R., Auerswald E. A., Kurz C., Zink B., Osterburg G., Schaller H., Sugimoto K., Sugisaki H., Okamoto T. Nucleotide sequence of bacteriophage fd DNA. Nucleic Acids Res. 1978 Dec;5(12):4495–4503. doi: 10.1093/nar/5.12.4495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boeke J. D., Model P. A prokaryotic membrane anchor sequence: carboxyl terminus of bacteriophage f1 gene III protein retains it in the membrane. Proc Natl Acad Sci U S A. 1982 Sep;79(17):5200–5204. doi: 10.1073/pnas.79.17.5200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boeke J. D., Model P., Zinder N. D. Effects of bacteriophage f1 gene III protein on the host cell membrane. Mol Gen Genet. 1982;186(2):185–192. doi: 10.1007/BF00331849. [DOI] [PubMed] [Google Scholar]
  4. Chang C. N., Model P., Blobel G. Membrane biogenesis: cotranslational integration of the bacteriophage f1 coat protein into an Escherichia coli membrane fraction. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1251–1255. doi: 10.1073/pnas.76.3.1251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grant R. A., Lin T. C., Konigsberg W., Webster R. E. Structure of the filamentous bacteriophage fl. Location of the A, C, and D minor coat proteins. J Biol Chem. 1981 Jan 10;256(1):539–546. [PubMed] [Google Scholar]
  6. Hill D. F., Petersen G. B. Nucleotide sequence of bacteriophage f1 DNA. J Virol. 1982 Oct;44(1):32–46. doi: 10.1128/jvi.44.1.32-46.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Humphreys G. O., Willshaw G. A., Anderson E. S. A simple method for the preparation of large quantities of pure plasmid DNA. Biochim Biophys Acta. 1975 Apr 2;383(4):457–463. doi: 10.1016/0005-2787(75)90318-4. [DOI] [PubMed] [Google Scholar]
  8. Konings R. N., Hulsebos T., Van den Hondel C. A. Identification and characterization of the in vitro synthesized gene products of bacteriophage M13. J Virol. 1975 Mar;15(3):570–584. doi: 10.1128/jvi.15.3.570-584.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Konings R. N. Synthesis of phage M13 specific proteins in a DNA-dependent cell-free system. FEBS Lett. 1973 Sep 1;35(1):155–160. doi: 10.1016/0014-5793(73)80600-3. [DOI] [PubMed] [Google Scholar]
  10. Konings R. N. Template function of restriction enzyme fragments of phage M13 replicative form DNA. Methods Enzymol. 1980;65(1):795–811. doi: 10.1016/s0076-6879(80)65074-5. [DOI] [PubMed] [Google Scholar]
  11. Konings R. N., Ward R., Francke B., Hofschneider P. H. Gene order of RNA bacteriophage M 12. Nature. 1970 May 16;226(5246):604–607. doi: 10.1038/226604a0. [DOI] [PubMed] [Google Scholar]
  12. Kyte J., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. doi: 10.1016/0022-2836(82)90515-0. [DOI] [PubMed] [Google Scholar]
  13. Lin T. C., Webster R. E., Konigsberg W. Isolation and characterization of the C and D proteins coded by gene IX and gene VI in the filamentous bacteriophage fl and fd. J Biol Chem. 1980 Nov 10;255(21):10331–10337. [PubMed] [Google Scholar]
  14. Makowski L., Caspar D. L., Marvin D. A. Filamentous bacteriophage Pf1 structure determined at 7A resolution by refinement of models for the alpha-helical subunit. J Mol Biol. 1980 Jun 25;140(2):149–181. doi: 10.1016/0022-2836(80)90101-1. [DOI] [PubMed] [Google Scholar]
  15. Marco R., Jazwinski S. M., Kornberg A. Binding, eclipse, and penetration of the filamentous bacteriophage M13 in intact and disrupted cells. Virology. 1974 Nov;62(1):209–223. doi: 10.1016/0042-6822(74)90316-x. [DOI] [PubMed] [Google Scholar]
  16. Marvin D. A., Wiseman R. L., Wachtel E. J. Filamentous bacterial viruses. XI. Molecular architecture of the class II (Pf1, Xf) virion. J Mol Biol. 1974 Jan 15;82(2):121–138. doi: 10.1016/0022-2836(74)90336-2. [DOI] [PubMed] [Google Scholar]
  17. Messing J., Vieira J. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982 Oct;19(3):269–276. doi: 10.1016/0378-1119(82)90016-6. [DOI] [PubMed] [Google Scholar]
  18. Newman J., Day L. A., Dalack G. W., Eden D. Hydrodynamic determination of molecular weight, dimensions, and structural parameters of Pf3 virus. Biochemistry. 1982 Jul 6;21(14):3352–3358. doi: 10.1021/bi00257a016. [DOI] [PubMed] [Google Scholar]
  19. Peterson C., Winter W. T., Dalack G. W., Day L. A. Structure of the filamentous bacteriophage, Pf3, by X-ray fiber diffraction. J Mol Biol. 1982 Dec 25;162(4):877–881. doi: 10.1016/0022-2836(82)90552-6. [DOI] [PubMed] [Google Scholar]
  20. Pratt D., Tzagoloff H., Beaudoin J. Conditional lethal mutants of the small filamentous coliphage M13. II. Two genes for coat proteins. Virology. 1969 Sep;39(1):42–53. doi: 10.1016/0042-6822(69)90346-8. [DOI] [PubMed] [Google Scholar]
  21. Rivera M. J., Smits M. A., Quint W., Schoenmakers J. G., Konings R. N. Expression of bacteriophage M13 DNA in vivo. Localization of the transcription initiation and termination signal of the mRNA coding for the major capsid protein. Nucleic Acids Res. 1978 Aug;5(8):2895–2912. doi: 10.1093/nar/5.8.2895. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Russel M., Model P. A mutation downstream from the signal peptidase cleavage site affects cleavage but not membrane insertion of phage coat protein. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1717–1721. doi: 10.1073/pnas.78.3.1717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Simons G. F., Konings R. N., Schoenmakers J. G. Genes VI, VII, and IX of phage M13 code for minor capsid proteins of the virion. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4194–4198. doi: 10.1073/pnas.78.7.4194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Simons G. F., Konings R. N., Schoenmakers J. G. Identification of two new capsid proteins in bacteriophage M13. FEBS Lett. 1979 Oct 1;106(1):8–12. doi: 10.1016/0014-5793(79)80683-3. [DOI] [PubMed] [Google Scholar]
  26. Staden R. A new computer method for the storage and manipulation of DNA gel reading data. Nucleic Acids Res. 1980 Aug 25;8(16):3673–3694. doi: 10.1093/nar/8.16.3673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Stanisich V. A. The properties and host range of male-specific bacteriophages of Pseudomonas aeruginosa. J Gen Microbiol. 1974 Oct;84(2):332–342. doi: 10.1099/00221287-84-2-332. [DOI] [PubMed] [Google Scholar]
  28. Stormo G. D., Schneider T. D., Gold L., Ehrenfeucht A. Use of the 'Perceptron' algorithm to distinguish translational initiation sites in E. coli. Nucleic Acids Res. 1982 May 11;10(9):2997–3011. doi: 10.1093/nar/10.9.2997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Sugimoto K., Sugisaki H., Okamoto T., Takanami M. Studies on bacteriophage fd DNA. IV. The sequence of messenger RNA for the major coat protein gene. J Mol Biol. 1977 Apr 25;111(4):487–507. doi: 10.1016/s0022-2836(77)80065-x. [DOI] [PubMed] [Google Scholar]
  30. Thomas G. J., Jr, Prescott B., Day L. A. Structure similarity, difference and variability in the filamentous viruses fd, If1, IKe, Pf1 and Xf. Investigation by laser Raman spectroscopy. J Mol Biol. 1983 Apr 5;165(2):321–356. doi: 10.1016/s0022-2836(83)80260-5. [DOI] [PubMed] [Google Scholar]
  31. Van Den Hondel C. A., Pennings L., Schoenmakers J. G. Restriction-enzyme-cleavage maps of bacteriophage M13. Existence of an intergenic region on the M13 genome. Eur J Biochem. 1976 Sep;68(1):55–70. doi: 10.1111/j.1432-1033.1976.tb10764.x. [DOI] [PubMed] [Google Scholar]
  32. Webster R. E., Grant R. A., Hamilton L. A. Orientation of the DNA in the filamentous bacteriophage f1. J Mol Biol. 1981 Oct 25;152(2):357–374. doi: 10.1016/0022-2836(81)90247-3. [DOI] [PubMed] [Google Scholar]
  33. Woolford J. L., Jr, Steinman H. M., Webster R. E. Adsorption protein of bacteriophage fl: solubilization in deoxycholate and localization in the fl virion. Biochemistry. 1977 Jun 14;16(12):2694–2700. doi: 10.1021/bi00631a017. [DOI] [PubMed] [Google Scholar]
  34. van Wezenbeek P. M., Hulsebos T. J., Schoenmakers J. G. Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd. Gene. 1980 Oct;11(1-2):129–148. doi: 10.1016/0378-1119(80)90093-1. [DOI] [PubMed] [Google Scholar]

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