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. 1983 Dec 10;11(23):8237–8251. doi: 10.1093/nar/11.23.8237

Nucleotide sequence and stability of the RNA component of RNase P from a temperature-sensitive mutant of E. coli.

H Sakamoto, N Kimura, F Nagawa, Y Shimura
PMCID: PMC326578  PMID: 6200826

Abstract

The gene coding for the RNA component of RNase P was cloned from a temperature-sensitive mutant of Escherichia coli defective in RNase P activity (ts709) and its parental wild-type strain (4273), and the complete nucleotide sequences of the gene and its flanking regions were determined. The 5'- and 3'-terminal sequences of the RNA component were determined and mapped on the DNA sequence. The mutant gene has GC-to-AT substitutions at positions corresponding to 89 and 365 nucleotides downstream from the 5' terminus of the RNA sequence. Comparing to the wild-type RNA, the mutant RNA is less stable and rapidly degraded in vivo and in vitro.

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