Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1983 Dec 10;11(23):8237–8251. doi: 10.1093/nar/11.23.8237

Nucleotide sequence and stability of the RNA component of RNase P from a temperature-sensitive mutant of E. coli.

H Sakamoto, N Kimura, F Nagawa, Y Shimura
PMCID: PMC326578  PMID: 6200826

Abstract

The gene coding for the RNA component of RNase P was cloned from a temperature-sensitive mutant of Escherichia coli defective in RNase P activity (ts709) and its parental wild-type strain (4273), and the complete nucleotide sequences of the gene and its flanking regions were determined. The 5'- and 3'-terminal sequences of the RNA component were determined and mapped on the DNA sequence. The mutant gene has GC-to-AT substitutions at positions corresponding to 89 and 365 nucleotides downstream from the 5' terminus of the RNA sequence. Comparing to the wild-type RNA, the mutant RNA is less stable and rapidly degraded in vivo and in vitro.

Full text

PDF
8237

Images in this article

8241Image
on p.8241
8245Image
on p.8245
8246Image
on p.8246
8247Image
on p.8247
8248Image
on p.8248

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altman S., Smith J. D. Tyrosine tRNA precursor molecule polynucleotide sequence. Nat New Biol. 1971 Sep 8;233(36):35–39. doi: 10.1038/newbio233035a0. [DOI] [PubMed] [Google Scholar]
  2. Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
  3. Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Donis-Keller H. Phy M: an RNase activity specific for U and A residues useful in RNA sequence analysis. Nucleic Acids Res. 1980 Jul 25;8(14):3133–3142. doi: 10.1093/nar/8.14.3133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hohn B. In vitro packaging of lambda and cosmid DNA. Methods Enzymol. 1979;68:299–309. doi: 10.1016/0076-6879(79)68021-7. [DOI] [PubMed] [Google Scholar]
  6. Ikemura T., Shimura Y., Sakano H., Ozeki H. Precursor molecules of Escherichia coli transfer RNAs accumulated in a temperature-sensitive mutant. J Mol Biol. 1975 Jul 25;96(1):69–86. doi: 10.1016/0022-2836(75)90182-5. [DOI] [PubMed] [Google Scholar]
  7. Jain S. K., Gurevitz M., Apirion D. A small RNA that complements mutants in the RNA processing enzyme ribonuclease P. J Mol Biol. 1982 Dec 15;162(3):515–533. doi: 10.1016/0022-2836(82)90386-2. [DOI] [PubMed] [Google Scholar]
  8. Jay E., Bambara R., Padmanabhan R., Wu R. DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping. Nucleic Acids Res. 1974 Mar;1(3):331–353. doi: 10.1093/nar/1.3.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kole R., Altman S. Reconstitution of RNase P activity from inactive RNA and protein. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3795–3799. doi: 10.1073/pnas.76.8.3795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kole R., Baer M. F., Stark B. C., Altman S. E. coli RNAase P has a required RNA component. Cell. 1980 Apr;19(4):881–887. doi: 10.1016/0092-8674(80)90079-3. [DOI] [PubMed] [Google Scholar]
  11. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  12. Motamedi H., Lee K., Nichols L., Schmidt F. J. An RNA species involved in Escherichia coli ribonuclease P activity. Gene cloning and effect on transfer RnA synthesis in vivo. J Mol Biol. 1982 Dec 15;162(3):535–550. doi: 10.1016/0022-2836(82)90387-4. [DOI] [PubMed] [Google Scholar]
  13. Nakajima N., Ozeki H., Shimura Y. In vitro transcription of the supB-E tRNA operon of Escherichia coli. Characterization of transcription products. J Biol Chem. 1982 Sep 25;257(18):11113–11120. [PubMed] [Google Scholar]
  14. Nakajima N., Ozeki H., Shimura Y. Organization and structure of an E. coli tRNA operon containing seven tRNA genes. Cell. 1981 Jan;23(1):239–249. doi: 10.1016/0092-8674(81)90288-9. [DOI] [PubMed] [Google Scholar]
  15. Ozeki H., Sakano H., Yamada S., Ikemura T., Shimura Y. Temperature-sensitive mutants of Escherichia coli defective in tRNA biosynthesis. Brookhaven Symp Biol. 1975 Jul;(26):89–105. [PubMed] [Google Scholar]
  16. Reed R. E., Baer M. F., Guerrier-Takada C., Donis-Keller H., Altman S. Nucleotide sequence of the gene encoding the RNA subunit (M1 RNA) of ribonuclease P from Escherichia coli. Cell. 1982 Sep;30(2):627–636. doi: 10.1016/0092-8674(82)90259-8. [DOI] [PubMed] [Google Scholar]
  17. Sakano H., Shimura Y. Characterization and in vitro processing of transfer RNA precursors accumulated in a temperature-sensitive mutant of Escherichia coli. J Mol Biol. 1978 Aug 15;123(3):287–326. doi: 10.1016/0022-2836(78)90082-7. [DOI] [PubMed] [Google Scholar]
  18. Sakano H., Yamada S., Ikemura T., Shimura Y., Ozeki H. Temperature sensitive mutants of Escherichia coli for tRNA synthesis. Nucleic Acids Res. 1974 Mar;1(3):355–371. doi: 10.1093/nar/1.3.355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sanger F., Brownlee G. G., Barrell B. G. A two-dimensional fractionation procedure for radioactive nucleotides. J Mol Biol. 1965 Sep;13(2):373–398. doi: 10.1016/s0022-2836(65)80104-8. [DOI] [PubMed] [Google Scholar]
  20. Schedl P., Primakoff P. Mutants of Escherichia coli thermosensitive for the synthesis of transfer RNA. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2091–2095. doi: 10.1073/pnas.70.7.2091. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shimura Y., Sakano H., Nagawa F. Specific ribonucleases involved in processing of tRNA precursors of Escherichia coli. Partial purification and some properties. Eur J Biochem. 1978 May;86(1):267–281. doi: 10.1111/j.1432-1033.1978.tb12308.x. [DOI] [PubMed] [Google Scholar]
  22. Stark B. C., Kole R., Bowman E. J., Altman S. Ribonuclease P: an enzyme with an essential RNA component. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3717–3721. doi: 10.1073/pnas.75.8.3717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES