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. 1983 Dec 10;11(23):8421–8429. doi: 10.1093/nar/11.23.8421

Stabilization of (dG-dC)n.(dG-dC)n in the Z conformation by a crosslinking reaction.

H Castleman, L H Hanau, B F Erlanger
PMCID: PMC326592  PMID: 6672768

Abstract

(dG-dC)n.(dG-dC) was converted to the Z conformer by heating in the presence of Mn++n. Reaction of this preparation with the crosslinking reagent, DL-diepoxybutane (DEB), stabilized this conformer so that it retained its structure even when returned to conditions that favored reversion to the B conformation. Treatment of the crosslinked Z conformer with periodate caused scission of the crosslink, allowing reversion to the B conformer. Reaction of (dG-dC)n.(dG-dC)n in the B conformation with DEB did not prevent conversion to the Z conformer in 4M NaC1; dialysis of the high salt solution against low ionic strength buffer allowed return to the B conformer. The Z in equilibrium B transitions were followed by circular dichroism studies and immunochemical procedures. The results suggest the feasibility of stabilizing Z sequences of DNA in chromatin by crosslinking, so that they could then be identified after DNA isolation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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