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. 1981 Mar 25;9(6):1291–1299. doi: 10.1093/nar/9.6.1291

Nucleotide sequence and structure of cytoplasmic 5S RNA and 5.8S RNA of Chlamydomonas reinhardii.

J L Darlix, J D Rochaix
PMCID: PMC326757  PMID: 7232217

Abstract

Three small RNAs of the cytoplasmic 8OS ribosomes of the green unicellular alga Chlamydomonas reinhardii have been sequenced. They include two species of ribosomal 5S RNA, a major and a minor one of 122 and 121 nucleotides respectively, which differ from each other by 17 bases, and also the ribosomal 5.8S RNA of 156 nucleotides. Novel structural features can be recognized in the 5S RNAs of C. reinhardii by a comparison with published 5S RNA sequences. In addition the secondary structure of these small RNA molecules has been examined using a newly developed method based on differential nuclease susceptibility.

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  1. Darlix J. L., Levray M., Bromley P. A., Spahr P. F. Characterization of the genomic RNA from a Rous sarcoma virus mutant temperature sensitive for cell transformation. Nucleic Acids Res. 1979 Feb;6(2):471–485. doi: 10.1093/nar/6.2.471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Darlix J. L. Stimultaneous purification of Escherichia coli termination factor rho, RNAase III and RNAase H. Eur J Biochem. 1975 Feb 21;51(2):369–376. doi: 10.1111/j.1432-1033.1975.tb03937.x. [DOI] [PubMed] [Google Scholar]
  3. Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dunn J. J., Anderson C. W., Atkins J. F., Bartelt D. C., Crockett W. C. Bacteriophages T7 and T3 as model systems for RNA synthesis and processing. Prog Nucleic Acid Res Mol Biol. 1976;19:263–273. doi: 10.1016/s0079-6603(08)60924-1. [DOI] [PubMed] [Google Scholar]
  5. Erdmann V. A. Structure and function of 5S and 5.8 S RNA. Prog Nucleic Acid Res Mol Biol. 1976;18:45–90. [PubMed] [Google Scholar]
  6. Ford P. J., Southern E. M. Different sequences for 5S RNA in kidney cells and ovaries of Xenopus laevis. Nat New Biol. 1973 Jan 3;241(105):7–12. doi: 10.1038/newbio241007a0. [DOI] [PubMed] [Google Scholar]
  7. Forget B. G., Weissman S. M. The nucleotide sequence of ribosomal 5 S ribonucleic acid from KB cells. J Biol Chem. 1969 Jun 25;244(12):3148–3165. [PubMed] [Google Scholar]
  8. Gerlach W. L., Dyer T. A. Sequence organization of the repeating units in the nucleus of wheat which contain 5S rRNA genes. Nucleic Acids Res. 1980 Nov 11;8(21):4851–4865. doi: 10.1093/nar/8.21.4851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hori H., Osawa S. Evolutionary change in 5S RNA secondary structure and a phylogenic tree of 54 5S RNA species. Proc Natl Acad Sci U S A. 1979 Jan;76(1):381–385. doi: 10.1073/pnas.76.1.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kelly J. M., Maden B. E. Chemical modification studies and the secondary structure of HeLa cell 5.8S rRNA. Nucleic Acids Res. 1980 Oct 10;8(19):4521–4534. doi: 10.1093/nar/8.19.4521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Korn L. J., Brown D. D. Nucleotide sequence of Xenopus borealis oocyte 5S DNA: comparison of sequences that flank several related eucaryotic genes. Cell. 1978 Dec;15(4):1145–1156. doi: 10.1016/0092-8674(78)90042-9. [DOI] [PubMed] [Google Scholar]
  12. Luoma G. A., Burns P. D., Bruce R. E., Marshall A. G. Melting of Saccharomyces cerevisiae 5S ribonucleic acid: ultraviolet absorption, circular dichroism, and 360-MHz proton nuclear magnetic resonance spectroscopy. Biochemistry. 1980 Nov 11;19(23):5456–5462. doi: 10.1021/bi00564a047. [DOI] [PubMed] [Google Scholar]
  13. MacKay R. M., Gray M. W., Doolittle W. F. Nucleotide sequence of Crithidia fasciculata cytosol 5S ribosomal ribonucleic acid. Nucleic Acids Res. 1980 Nov 11;8(21):4911–4917. doi: 10.1093/nar/8.21.4911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Min Jou W., Haegeman G., Ysebaert M., Fiers W. Nucleotide sequence of the gene coding for the bacteriophage MS2 coat protein. Nature. 1972 May 12;237(5350):82–88. doi: 10.1038/237082a0. [DOI] [PubMed] [Google Scholar]
  15. Nazar R. N., Sitz T. O., Busch H. Structural analyses of mammalian ribosomal ribonucleic acid and its precursors. Nucleotide sequence of ribosomal 5.8 S ribonucleic acid. J Biol Chem. 1975 Nov 25;250(22):8591–8597. [PubMed] [Google Scholar]
  16. Nishikawa K., Takemura S. Nucleotide sequence of 5 S RNA from Torulopsis utilis. FEBS Lett. 1974 Mar 15;40(1):106–109. doi: 10.1016/0014-5793(74)80904-x. [DOI] [PubMed] [Google Scholar]
  17. Peattie D. A. Direct chemical method for sequencing RNA. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1760–1764. doi: 10.1073/pnas.76.4.1760. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pilly D., Niemeyer A., Schmidt M., Bargetzi J. P. Enzymes for RNA sequence analysis. Preparation and specificity of exoplasmodial ribonucleases I and II from Physarum polycephalum. J Biol Chem. 1978 Jan 25;253(2):437–445. [PubMed] [Google Scholar]
  19. Randerath K., Gupta R. C., Randerath E. 3H and 32P derivative methods for base composition and sequence analysis of RNA. Methods Enzymol. 1980;65(1):638–680. doi: 10.1016/s0076-6879(80)65065-4. [DOI] [PubMed] [Google Scholar]
  20. Rensing U. F., Schoenmakers G. G. A sequence of 50 nucleotides from coliphage R17 RNA. Eur J Biochem. 1973 Feb 15;33(1):8–18. doi: 10.1111/j.1432-1033.1973.tb02648.x. [DOI] [PubMed] [Google Scholar]
  21. Rubin G. M. The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid. J Biol Chem. 1973 Jun 10;248(11):3860–3875. [PubMed] [Google Scholar]
  22. Simoncsits A., Brownlee G. G., Brown R. S., Rubin J. R., Guilley H. New rapid gel sequencing method for RNA. Nature. 1977 Oct 27;269(5631):833–836. doi: 10.1038/269833a0. [DOI] [PubMed] [Google Scholar]
  23. Williamson R., Brownlee G. G. The sequence of 5S ribosomal RNA from two mouse cell lines. FEBS Lett. 1969 Jun;3(5):306–308. doi: 10.1016/0014-5793(69)80163-8. [DOI] [PubMed] [Google Scholar]

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