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. 1981 May 25;9(10):2325–2333. doi: 10.1093/nar/9.10.2325

The sequence of the ribosomal 16S RNA from Proteus vulgaris. Sequence comparison with E. coli 16S RNA and its use in secondary model building.

P Carbon, J P Ebel, C Ehresmann
PMCID: PMC326848  PMID: 7019853

Abstract

The complete nucleotide sequence of the 16S RNA from Proteus vulgaris has been determined. The molecule (1544 nucleotides) shows 93% homology with the sequence of E. coli 16S RNA. Six methylated nucleotides have been localized in positions homologous to those observed in the E. coli RNA molecule. Both E. coli and P. vulgaris 16S RNA chains can be folded up into a common secondary structure scheme. Comparative sequence analysis of the two molecules has provided a valuable contribution to 16S RNA secondary structure model building.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Branlant C., Krol A., Machatt A., Ebel J. P. The secondary structure of the protein L1 binding region of ribosomal 23S RNA. Homologies with putative secondary structures of the L11 mRNA and of a region of mitochondrial 16S rRNA. Nucleic Acids Res. 1981 Jan 24;9(2):293–307. doi: 10.1093/nar/9.2.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4801–4805. doi: 10.1073/pnas.75.10.4801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Daya-Grosjean L., Geisser M., Stöffler G., Garret R. A. Heterologous protein-RNA interactions in bacterial ribosomes. FEBS Lett. 1973 Nov 15;37(1):17–20. doi: 10.1016/0014-5793(73)80416-8. [DOI] [PubMed] [Google Scholar]
  4. Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ehresmann C., Stiegler P., Fellner P., Ebel J. P. The determination of the primary structure of the 16S ribosomal RNA of Escherichia coli. III. Further studies. Biochimie. 1975;57(6-7):711–748. doi: 10.1016/s0300-9084(75)80047-2. [DOI] [PubMed] [Google Scholar]
  6. England T. E., Uhlenbeck O. C. 3'-terminal labelling of RNA with T4 RNA ligase. Nature. 1978 Oct 12;275(5680):560–561. doi: 10.1038/275560a0. [DOI] [PubMed] [Google Scholar]
  7. Fellner P. Nucleotide sequences from specific areas of the 16S and 23S ribosomal RNAs of E. coli. Eur J Biochem. 1969 Nov;11(1):12–27. doi: 10.1111/j.1432-1033.1969.tb00733.x. [DOI] [PubMed] [Google Scholar]
  8. Fischel J. L., Krol A., Ehresmann C., Fellner P., Ebel J. P. Comparative study of the 16S RNA's of Escherichia coli and Proteus vulgaris. Biochimie. 1975;57(8):885–897. doi: 10.1016/s0300-9084(75)80211-2. [DOI] [PubMed] [Google Scholar]
  9. Glotz C., Brimacombe R. An experimentally-derived model for the secondary structure of the 16S ribosomal RNA from Escherichia coli. Nucleic Acids Res. 1980 Jun 11;8(11):2377–2395. doi: 10.1093/nar/8.11.2377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Krupp G., Gross H. J. Rapid RNA sequencing: nucleases from Staphylococcus aureus and Neurospora crassa discriminate between uridine and cytidine. Nucleic Acids Res. 1979 Aug 10;6(11):3481–3490. doi: 10.1093/nar/6.11.3481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Peattie D. A. Direct chemical method for sequencing RNA. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1760–1764. doi: 10.1073/pnas.76.4.1760. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shinagawa M., Padmanabhan R. Inhibition of a nuclease contaminant in the commercial preparations of Escherichia coli alkaline phosphatase. Anal Biochem. 1979 Jun;95(2):458–464. doi: 10.1016/0003-2697(79)90756-5. [DOI] [PubMed] [Google Scholar]
  14. Stiegler P., Carbon P., Zuker M., Ebel J. P., Ehresmann C. Structure secondaire et topographie du RNA ribosomique 16S d'Escherichia coli. C R Seances Acad Sci D. 1980 Dec 8;291(12):937–940. [PubMed] [Google Scholar]
  15. Vasilenko S. K., Ryte V. C. [Isolation of highly purified ribonuclease from cobra (Naja oxiana) venom]. Biokhimiia. 1975 May-Jun;40(3):578–583. [PubMed] [Google Scholar]
  16. Woese C. R., Fox G. E., Zablen L., Uchida T., Bonen L., Pechman K., Lewis B. J., Stahl D. Conservation of primary structure in 16S ribosomal RNA. Nature. 1975 Mar 6;254(5495):83–86. doi: 10.1038/254083a0. [DOI] [PubMed] [Google Scholar]
  17. Woese C. R., Magrum L. J., Gupta R., Siegel R. B., Stahl D. A., Kop J., Crawford N., Brosius J., Gutell R., Hogan J. J. Secondary structure model for bacterial 16S ribosomal RNA: phylogenetic, enzymatic and chemical evidence. Nucleic Acids Res. 1980 May 24;8(10):2275–2293. doi: 10.1093/nar/8.10.2275. [DOI] [PMC free article] [PubMed] [Google Scholar]

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