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. 1982 Oct 25;10(20):6521–6530. doi: 10.1093/nar/10.20.6521

A new restriction endonuclease from Citrobacter freundii

AA Janulaitis 1, PS Stakenas 1, EN Lebedenko *, YuA Berlin *
PMCID: PMC326941  PMID: 6294607

Abstract

CfrI, a new restriction endonuclease of unique substrate specificity, has been isolated from a Citrobacter freundii strain. The enzyme recognizes a degenerated sequence PyGGCCPu in double-strand DNA and cleaves it between Py and G residues to yield 5′ -protruding tetranucleotide ends GGCC.

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Selected References

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