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. 1982 Oct 25;10(20):6531–6539. doi: 10.1093/nar/10.20.6531

E. coli initiator tRNA analogs with different nucleotides in the discriminator base position.

H Uemura, M Imai, E Ohtsuka, M Ikehara, D Söll
PMCID: PMC326942  PMID: 6294608

Abstract

The effect of base changes at the fourth position from the 3'-terminus of Escherichia coli initiator tRNAMet has been studied to test the 'discriminator hypothesis' which proposed that the nucleotide in this position might have a role in the specificity of the aminoacylation reaction. E. coli initiator tRNA lacking the 3'-terminal tetranucleotide was prepared by partial digestion with S1 nuclease. To construct tRNA analogs with different bases in the fourth position this truncated tRNA was joined by RNA ligase to each of four chemically synthesized 2',3'-ethoxy-methylidene tetranucleotides pACCA(em), pCCCA(em), pGCCA(em), and pUCCA(em). In vitro aminoacylation studies showed that all four molecules accepted methionine, albeit with different Vmax values.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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