Abstract
The cytoplasmic initiator tRNA from the green alga Scenedesmus obliquus has been purified and its sequence shown to be p A G C U G A G-U m G m G C G C A G D G G A A G C G psi m G A psi G G G C U C A U t A A--C C C A U A G m G D m C A C A G G A U C G m A A A C C U Gm U C U C A--G C U A C C A-O H. The sequence has been deduced and confirmed using several different P-post labelling techniques. The sequence is similar to those of other eukaryotic cytoplasmic initiator tRNAs and it has the sequence G A U C in place of the usual G T psi C. Although it resembles lower eukaryotic species in having a U preceding the anticodon and a modified G in the T psi C stem, in overall homology it is closer to the higher eukaryotic than to the fungal initiator tRNAs.
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- Brown R. S., Rubin J. R., Rhodes D., Guilley H., Simoncsits A., Brownlee G. G. The nucleoside sequence of tyrosine tRNA from Bacillus stearothermophilus. Nucleic Acids Res. 1978 Jan;5(1):23–36. doi: 10.1093/nar/5.1.23. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fitch W. M., Margoliash E. Construction of phylogenetic trees. Science. 1967 Jan 20;155(3760):279–284. doi: 10.1126/science.155.3760.279. [DOI] [PubMed] [Google Scholar]
- Gauss D. H., Grüter F., Sprinzl M. Compilation of tRNA sequences. Nucleic Acids Res. 1979 Jan;6(1):r1–r19. [PMC free article] [PubMed] [Google Scholar]
- Gillam I., Millward S., Blew D., von Tigerstrom M., Wimmer E., Tener G. M. The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose. Biochemistry. 1967 Oct;6(10):3043–3056. doi: 10.1021/bi00862a011. [DOI] [PubMed] [Google Scholar]
- Gillum A. M., Hecker L. I., Silberklang M., Schwartzbach S. D., RajBhandary U. L., Barnett W. E. Nucleotide sequence of Neurospora crassa cytoplasmic initiator tRNA. Nucleic Acids Res. 1977 Dec;4(12):4109–4131. doi: 10.1093/nar/4.12.4109. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gillum A. M., Roe B. A., Anandaraj M. P., RajBhandary U. L. Nucleotide sequence of human placenta cytoplasmic initiator tRNA. Cell. 1975 Nov;6(3):407–413. doi: 10.1016/0092-8674(75)90190-7. [DOI] [PubMed] [Google Scholar]
- Gillum A. M., Urquhart N., Smith M., RajBhandary U. L. Nucleotide sequence of salmon testes and salmon liver cytoplasmic initiator tRNA. Cell. 1975 Nov;6(3):395–405. doi: 10.1016/0092-8674(75)90189-0. [DOI] [PubMed] [Google Scholar]
- Jay F. T., Coultas C., Jones D. S. The use of a dipolar ion-exchanger for the fractionation of transfer ribonucleic acid. Nucleic Acids Res. 1976 Jan;3(1):177–190. doi: 10.1093/nar/3.1.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuchino Y., Kato M., Sugisaki H., Nishimura S. Nucleotide sequence of starfish initiator tRNA. Nucleic Acids Res. 1979 Aug 10;6(11):3459–3469. doi: 10.1093/nar/6.11.3459. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lockard R. E., Alzner-Deweerd B., Heckman J. E., MacGee J., Tabor M. W., RajBhandary U. L. Sequence analysis of 5'[32P] labeled mRNA and tRNA using polyacrylamide gel electrophoresis. Nucleic Acids Res. 1978 Jan;5(1):37–56. doi: 10.1093/nar/5.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lu L. W., Chiang G. H., Tseng W. C., Randerath K. Effects of 5-fluorouridine on modified nucleosides in mouse liver transfer RNA. Biochem Biophys Res Commun. 1976 Dec 20;73(4):1075–1082. doi: 10.1016/0006-291x(76)90233-3. [DOI] [PubMed] [Google Scholar]
- Martin M. G., Reese C. B. Some aspects of the chemistry of N(1)- and N(6)-dimethylallyl derivatives of adenosine and adenine. J Chem Soc Perkin 1. 1968;14:1731–1738. doi: 10.1039/j39680001731. [DOI] [PubMed] [Google Scholar]
- Old J. M., Jones D. S. The aminoacylation of transfer ribonucleic acid. Inhibitory effects of some amino acid analogues with altered side chains. Biochem J. 1976 Dec 1;159(3):503–511. doi: 10.1042/bj1590503. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pearson R. L., Weiss J. F., Kelmers A. D. Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns. Biochim Biophys Acta. 1971 Feb 11;228(3):770–774. doi: 10.1016/0005-2787(71)90748-9. [DOI] [PubMed] [Google Scholar]
- Porath J., Fornstedt N. Group fractionation of plasma proteins on dipolar ion exchangers. J Chromatogr. 1970 Sep 23;51(3):479–489. doi: 10.1016/s0021-9673(01)96895-5. [DOI] [PubMed] [Google Scholar]
- Randerath E., Yu C. T., Randerath K. Base analysis of ribopolynucleotides by chemical tritium labeling: a methodological study with model nucleosides and purified tRNA species. Anal Biochem. 1972 Jul;48(1):172–198. doi: 10.1016/0003-2697(72)90181-9. [DOI] [PubMed] [Google Scholar]
- Randerath K., Randerath E., Chia L. S., Nowak B. J. Base analysis of ribopolynucleotides by chemical tritium labeling: an improved mapping procedure for nucleoside trialcohols. Anal Biochem. 1974 May;59(1):263–271. doi: 10.1016/0003-2697(74)90032-3. [DOI] [PubMed] [Google Scholar]
- Sanger F., Donelson J. E., Coulson A. R., Kössel H., Fischer D. Use of DNA polymerase I primed by a synthetic oligonucleotide to determine a nucleotide sequence in phage fl DNA. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1209–1213. doi: 10.1073/pnas.70.4.1209. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Silberklang M., Gillum A. M., RajBhandary U. L. The use of nuclease P1 in sequence analysis of end group labeled RNA. Nucleic Acids Res. 1977 Dec;4(12):4091–4108. doi: 10.1093/nar/4.12.4091. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Silberklang M., Gillum A. M., RajBhandary U. L. Use of in vitro 32P labeling in the sequence analysis of nonradioactive tRNAs. Methods Enzymol. 1979;59:58–109. doi: 10.1016/0076-6879(79)59072-7. [DOI] [PubMed] [Google Scholar]
- Silberklang M., Prochiantz A., Haenni A. L., Rajbhandary U. L. Studies on the sequence of the 3'-terminal region of turnip-yellow-mosaic-virus RNA. Eur J Biochem. 1977 Feb;72(3):465–478. doi: 10.1111/j.1432-1033.1977.tb11270.x. [DOI] [PubMed] [Google Scholar]
- Simoncsits A., Brownlee G. G., Brown R. S., Rubin J. R., Guilley H. New rapid gel sequencing method for RNA. Nature. 1977 Oct 27;269(5631):833–836. doi: 10.1038/269833a0. [DOI] [PubMed] [Google Scholar]
- Simsek M., RajBhandary U. L. The primary structure of yeast initiator transfer ribonucleic acid. Biochem Biophys Res Commun. 1972 Oct 17;49(2):508–515. doi: 10.1016/0006-291x(72)90440-8. [DOI] [PubMed] [Google Scholar]
- Stanley J., Vassilenko S. A different approach to RNA sequencing. Nature. 1978 Jul 6;274(5666):87–89. doi: 10.1038/274087a0. [DOI] [PubMed] [Google Scholar]
- Székely M., Sanger F. Use of polynucleotide kinase in fingerprinting non-radioactive nucleic acids. J Mol Biol. 1969 Aug 14;43(3):607–617. doi: 10.1016/0022-2836(69)90362-3. [DOI] [PubMed] [Google Scholar]
- Yamashiro-Matsumura S., Takemura S. The primary structure of cytoplasmic initiator transfer ribonucleic acid from Torulopsis utilis. J Biochem. 1979 Aug;86(2):335–346. doi: 10.1093/oxfordjournals.jbchem.a132531. [DOI] [PubMed] [Google Scholar]