Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1981 Jul 10;9(13):3061–3075. doi: 10.1093/nar/9.13.3061

Cloning of a new mouse foetal beta-globin mRNA sequence.

N A Affara, P S Goldfarb, K Vass, A Lyons, P R Harrison
PMCID: PMC327331  PMID: 7024908

Abstract

A novel globin cDNA recombinant (pFG5) has been isolated from a 14-15 day Porton mouse foetal liver cDNA library. It codes for a beta-like globin mRNA expressed in foetal liver-derived erythroblasts and erythrocytes but not in adult reticulocytes nor in yolk sac derived nucleated erythrocytes. It is also found in Friend cells induced to differentiate by DMSO. The nucleotide sequence of pFG5 confirms that it does not code for the beta major or beta minor globin chains nor the embryonic epsilon Y2 globin chain; but it is identical to the published partial sequence of the epsilon Y3 globin gene over the region of overlap (78 nucleotides).

Full text

PDF
3070

Images in this article

3067Image
on p.3067
3070Image
on p.3070

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Affara N. A., Jacquet M., Jakob H., Jacob F., Gros F. Comparison of polysomal polyadenylated RNA from embryonal carcinoma and committed myogenic and erythropoietic cell lines. Cell. 1977 Oct;12(2):509–520. doi: 10.1016/0092-8674(77)90127-1. [DOI] [PubMed] [Google Scholar]
  2. Affara N., Daubas P. Regulation of a group of abundant mRNA sequences during Friend cell differentiation. Dev Biol. 1979 Sep;72(1):110–125. doi: 10.1016/0012-1606(79)90102-7. [DOI] [PubMed] [Google Scholar]
  3. Covey C., Richardson D., Carbon J. A method for the deletion of restriction sites in bacterial plasmid deoxyribonucleic acid. Mol Gen Genet. 1976 May 7;145(2):155–158. doi: 10.1007/BF00269587. [DOI] [PubMed] [Google Scholar]
  4. Fantoni A., Bank A., Marks P. A. Globin composition and synthesis of hemoglobins in developing fetal mice erythroid cells. Science. 1967 Sep 15;157(3794):1327–1329. doi: 10.1126/science.157.3794.1327. [DOI] [PubMed] [Google Scholar]
  5. Fantoni A., Bozzoni I., Ullu E., Farace M. G. Construction of a recombinant bacterial plasmid containing DNA sequences for a mouse embryonic globin chain. Nucleic Acids Res. 1979 Aug 10;6(11):3505–3517. doi: 10.1093/nar/6.11.3505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fritsch E. F., Lawn R. M., Maniatis T. Molecular cloning and characterization of the human beta-like globin gene cluster. Cell. 1980 Apr;19(4):959–972. doi: 10.1016/0092-8674(80)90087-2. [DOI] [PubMed] [Google Scholar]
  7. Gilman J. G. Mouse haemoglobin Beta chains. Sequence data on embryonic y chain and genetic linkage of the Y-chain locus to the adult beta-chain locus Hbb. Biochem J. 1976 May 1;155(2):231–241. doi: 10.1042/bj1550231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gilman J. G. Mouse haemoglobin beta chains. Comparative sequence data on adult major and minor beta chains from two species, Mus musculus and Mus cervicolor. Biochem J. 1976 Oct 1;159(1):43–53. doi: 10.1042/bj1590043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gilman J. G., Smithies O. Fetal hemoglobin variants in mice. Science. 1968 May 24;160(3830):885–886. doi: 10.1126/science.160.3830.885. [DOI] [PubMed] [Google Scholar]
  10. Hoeijmakers J. H., Borst P., van den Burg J., Weissmann C., Cross G. A. The isolation of plasmids containing DNA complementary to messenger RNA for variant surface glycoproteins of Trypanosoma brucei. Gene. 1980 Mar;8(4):391–417. doi: 10.1016/0378-1119(80)90043-8. [DOI] [PubMed] [Google Scholar]
  11. Jahn C. L., Hutchison C. A., 3rd, Phillips S. J., Weaver S., Haigwood N. L., Voliva C. F., Edgell M. H. DNA sequence organization of the beta-globin complex in the BALB/c mouse. Cell. 1980 Aug;21(1):159–168. doi: 10.1016/0092-8674(80)90123-3. [DOI] [PubMed] [Google Scholar]
  12. Jeffreys A. J., Wilson V., Wood D., Simons J. P., Kay R. M., Williams J. G. Linkage of adult alpha- and beta-globin genes in X. laevis and gene duplication by tetraploidization. Cell. 1980 Sep;21(2):555–564. doi: 10.1016/0092-8674(80)90493-6. [DOI] [PubMed] [Google Scholar]
  13. Konkel D. A., Maizel J. V., Jr, Leder P. The evolution and sequence comparison of two recently diverged mouse chromosomal beta--globin genes. Cell. 1979 Nov;18(3):865–873. doi: 10.1016/0092-8674(79)90138-7. [DOI] [PubMed] [Google Scholar]
  14. Kraus L. M., Rasad A., Oba Y., Patterson M. T. Mouse fetal hemoglobin. Ann N Y Acad Sci. 1974 Nov 29;241(0):683–690. doi: 10.1111/j.1749-6632.1974.tb21923.x. [DOI] [PubMed] [Google Scholar]
  15. Lacy E., Hardison R. C., Quon D., Maniatis T. The linkage arrangement of four rabbit beta-like globin genes. Cell. 1979 Dec;18(4):1273–1283. doi: 10.1016/0092-8674(79)90238-1. [DOI] [PubMed] [Google Scholar]
  16. Marks P. A., Rifkind R. A. Protein synthesis: its control in erythropoiesis. Science. 1972 Mar 3;175(4025):955–961. doi: 10.1126/science.175.4025.955. [DOI] [PubMed] [Google Scholar]
  17. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  18. Steinheider G., Melderis H., Ostertag W. Embryonic epsilon chains of mice and rabbits. Nature. 1975 Oct 23;257(5528):714–716. doi: 10.1038/257714a0. [DOI] [PubMed] [Google Scholar]
  19. Stern R. H., Russell E. S., Taylor B. A. Strain distribution and linkage relationship of a mouse embryonic hemoglobin variant. Biochem Genet. 1976 Apr;14(3-4):373–381. doi: 10.1007/BF00484775. [DOI] [PubMed] [Google Scholar]
  20. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tushinski R. J., Sussman P. M., Yu L. Y., Bancroft F. C. Pregrowth hormone messenger RNA: glucocorticoid induction and identification in rat pituitary cells. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2357–2361. doi: 10.1073/pnas.74.6.2357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Whitney J. B., 3rd Differential control of the synthesis of two hemoglobin beta chains in normal mice. Cell. 1977 Dec;12(4):863–871. doi: 10.1016/0092-8674(77)90150-7. [DOI] [PubMed] [Google Scholar]
  23. Whitney J. B., 3rd, McFarland E. C., Russell E. S. Interconversion of mouse adult hemoglobin and a neonatal spleen fast hemoglobin band. Dev Biol. 1978 Jul;65(1):233–237. doi: 10.1016/0012-1606(78)90193-8. [DOI] [PubMed] [Google Scholar]
  24. Wu N. C., Sikkema D. A., Zucker R. M. Mouse fetal hemoglobin. Biochim Biophys Acta. 1978 Sep 26;536(1):306–311. doi: 10.1016/0005-2795(78)90078-8. [DOI] [PubMed] [Google Scholar]
  25. Wu N. C., Zucker R. M. Mouse fetal hemoglobin synthesis in murine erythroleukemic cells. FEBS Lett. 1979 Mar 15;99(2):299–302. doi: 10.1016/0014-5793(79)80977-1. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES