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. 1981 Jul 24;9(14):3365–3377. doi: 10.1093/nar/9.14.3365

A promoter of pBR322 activated by cAMP receptor protein.

C Queen, M Rosenberg
PMCID: PMC327357  PMID: 6269081

Abstract

We have demonstrated in vitro the existence on the plasmid pBR322 of a promoter signal that is strictly dependent on cAMP and its receptor protein CRP. Transcription initiates with pppG at nucleotide 2270 and proceeds counterclockwise on the standard pBR322 map. DNase protection studies show that CRP selectively binds to the -35 region of the promoter. This region exhibits strong structural homologies to the binding sites of other CRP-dependent promoters.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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