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. 1981 Aug 25;9(16):4087–4098. doi: 10.1093/nar/9.16.4087

pUR222, a vector for cloning and rapid chemical sequencing of DNA.

U Rüther, M Koenen, K Otto, B Müller-Hill
PMCID: PMC327416  PMID: 6272193

Abstract

A multipurpose plasmid, pUR222, was constructed. It contains six unique cloning sites (PstI, SalI, AccI, HindII, BamHI and EcoRI) in a small region of its lac Z-gene part. Insertion of foreign DNA into the plasmid can be easily detected. Bacteria harbouring recombinant plasmids generally give rise to white colonies, while those containing only vector DNA form blue colonies on indicator plates. Plasmid DNA purified by a rapid method (Birnboim, H.C. and Doly, J. (1979) Nucl. Acids. Res. 7, 1513-1523) can be used for chemical sequencing of the cloned insert DNA. Labeled fragments need not be isolated after cutting with the proper restriction enzymes and are treated directly according to the sequencing protocol of Maxam and Gilbert.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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