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. 1979 Apr;6(4):1417–1431. doi: 10.1093/nar/6.4.1417

DNA replication in Physarum polycephalum: electron microscopic and autoradiographic analysis of replicating DNA from defined stages of the S-period.

S Funderud, R Andreassen, F Haugli
PMCID: PMC327780  PMID: 450701

Abstract

Electron microscopic and autoradiographic analysis of replicating DNA from Physarum showed that replication occurs at a rate of 0.4 micron/min/per replicon and that replicons of size 10--15 mu occur in temporal clusters with an average of about 4 replicons per cluster. These results are compared with previous hydrodynamic measurements and with those obtained in other organisms.

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Selected References

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  1. Blumenthal A. B., Kriegstein H. J., Hogness D. S. The units of DNA replication in Drosophila melanogaster chromosomes. Cold Spring Harb Symp Quant Biol. 1974;38:205–223. doi: 10.1101/sqb.1974.038.01.024. [DOI] [PubMed] [Google Scholar]
  2. Edenberg H. J., Huberman J. A. Eukaryotic chromosome replication. Annu Rev Genet. 1975;9:245–284. doi: 10.1146/annurev.ge.09.120175.001333. [DOI] [PubMed] [Google Scholar]
  3. Funderud S., Andreassen R., Haugli F. DNA replication in Physarum polycephalum: UV photolysis of maturing 5-bromo-deoxyuridine substituted DNA. Nucleic Acids Res. 1978 Sep;5(9):3303–3313. doi: 10.1093/nar/5.9.3303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Funderud S., Andreassen R., Haugli F. DNA replication in Physarum polycephalum: bidirectional replication of DNA within replicons. Nucleic Acids Res. 1978 Mar;5(3):713–721. doi: 10.1093/nar/5.3.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Funderud S., Haugli F. DNA replication in Physarum polycephalum: characterization of DNA replication products made in vivo in the presence of cycloheximide in strains sensitive and resistant to cycloheximide. Nucleic Acids Res. 1977 Feb;4(2):405–413. doi: 10.1093/nar/4.2.405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Funderud S., Haugli F. DNA replication in Physarum polycephalum: characterization of replication products in vivo. Nucleic Acids Res. 1975 Aug;2(8):1381–1390. doi: 10.1093/nar/2.8.1381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Funderud S., Haugli F. DNA replication in Physarum polycephalum: characterization of replication products made in isolated nuclei. Biochem Biophys Res Commun. 1977 Feb 7;74(3):941–948. doi: 10.1016/0006-291x(77)91609-6. [DOI] [PubMed] [Google Scholar]
  8. Hand R. Regulation of DNA replication on subchromosomal units of mammalian cells. J Cell Biol. 1975 Jan;64(1):89–97. doi: 10.1083/jcb.64.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hand R., Tamm I. Initiation of DNA replication in mammalian cells and its inhibition by reovirus infection. J Mol Biol. 1974 Jan 15;82(2):175–183. doi: 10.1016/0022-2836(74)90339-8. [DOI] [PubMed] [Google Scholar]
  10. Hori T., Lark K. G. Effect of puromycin on DNA replication in Chinese hamster cells. J Mol Biol. 1973 Jul 5;77(3):391–404. doi: 10.1016/0022-2836(73)90446-4. [DOI] [PubMed] [Google Scholar]
  11. Housman D., Huberman J. A. Changes in the rate of DNA replication fork movement during S phase in mammalian cells. J Mol Biol. 1975 May 15;94(2):173–181. doi: 10.1016/0022-2836(75)90076-5. [DOI] [PubMed] [Google Scholar]
  12. Huberman J. A., Riggs A. D. On the mechanism of DNA replication in mammalian chromosomes. J Mol Biol. 1968 Mar 14;32(2):327–341. doi: 10.1016/0022-2836(68)90013-2. [DOI] [PubMed] [Google Scholar]
  13. Kowalski J., Cheevers W. P. Synthesis of high molecular weight DNA strands during S phase. J Mol Biol. 1976 Jul 5;104(3):603–615. doi: 10.1016/0022-2836(76)90123-6. [DOI] [PubMed] [Google Scholar]
  14. Mohberg J., Rusch H. P. Isolation and DNA content of nuclei of Physarum polycephalum. Exp Cell Res. 1971 Jun;66(2):305–316. doi: 10.1016/0014-4827(71)90682-3. [DOI] [PubMed] [Google Scholar]
  15. Newlon C. S., Petes T. D., Hereford L. M., Fangman W. L. Replication of yeast chromosomal DNA. Nature. 1974 Jan 4;247(5435):32–35. doi: 10.1038/247032a0. [DOI] [PubMed] [Google Scholar]
  16. Planck S. R., Mueller G. C. Deoxyribonucleic acid chain growth and organization of replicating units in HeLa cells. Biochemistry. 1977 May 3;16(9):1808–1813. doi: 10.1021/bi00628a007. [DOI] [PubMed] [Google Scholar]
  17. Stimac E., Housman D., Huberman J. A. Effects of inhibition of protein synthesis on DNA replication in cultured mammalian cells. J Mol Biol. 1977 Sep 25;115(3):485–511. doi: 10.1016/0022-2836(77)90167-x. [DOI] [PubMed] [Google Scholar]
  18. Zimm B. H., Schumaker V. N., Zimm C. B. Anomalies in sedimentation. V. Chains at high fields, practical consequences. Biophys Chem. 1976 Jul;5(1-2):265–270. doi: 10.1016/0301-4622(76)80039-7. [DOI] [PubMed] [Google Scholar]

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