Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1979 Apr;6(4):1495–1508. doi: 10.1093/nar/6.4.1495

Operators and promoters in the OR region of phage 434.

V Pirrotta
PMCID: PMC327785  PMID: 450705

Abstract

The OR operator region of phage 434 contains three 14 bp blocks with sequence ACAAGA-A--TTGT which are presumed to be the 434 repressor recognition sites. Operator constitutive mutations are located in two of these blocks, while a mutation affecting repressor levels in the lysogenic state is located in the third. Two transcripts obtained in vitro, one leftwards and one rightwards, are tentatively identified as the Prm and PR transcription starts. the arrangement of the 434 operator region appears to be very similar to that of the OR of phage lambda.

Full text

PDF
1501

Images in this article

1499Image
on p.1499
1502Image
on p.1502
1503Image
on p.1503
1504Image
on p.1504

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Jay E., Bambara R., Padmanabhan R., Wu R. DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping. Nucleic Acids Res. 1974 Mar;1(3):331–353. doi: 10.1093/nar/1.3.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. KAISER A. D., JACOB F. Recombination between related temperate bacteriophages and the genetic control of immunity and prophage localization. Virology. 1957 Dec;4(3):509–521. doi: 10.1016/0042-6822(57)90083-1. [DOI] [PubMed] [Google Scholar]
  3. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Meyer B. J., Kleid D. G., Ptashne M. Lambda repressor turns off transcription of its own gene. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4785–4789. doi: 10.1073/pnas.72.12.4785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Murray K., Murray N. E. Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli. J Mol Biol. 1975 Nov 5;98(3):551–564. doi: 10.1016/s0022-2836(75)80086-6. [DOI] [PubMed] [Google Scholar]
  6. Pastrana R., Brammar W. J. Control of cI gene expression in bacteriophage lambda imm434, studied in an immunity/trp fusion made in vitro. Mol Gen Genet. 1976 Jul 23;146(2):191–198. doi: 10.1007/BF00268088. [DOI] [PubMed] [Google Scholar]
  7. Pirrotta V., Ptashne M. Isolation of the 434 phage repressor. Nature. 1969 May 10;222(5193):541–544. doi: 10.1038/222541a0. [DOI] [PubMed] [Google Scholar]
  8. Pirrotta V. Sequence of the OR operator of phage lambda. Nature. 1975 Mar 13;254(5496):114–117. doi: 10.1038/254114a0. [DOI] [PubMed] [Google Scholar]
  9. Pirrotta V. The lambda repressor and its action. Curr Top Microbiol Immunol. 1976;74:21–54. doi: 10.1007/978-3-642-66336-9_2. [DOI] [PubMed] [Google Scholar]
  10. Pribnow D. Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter. Proc Natl Acad Sci U S A. 1975 Mar;72(3):784–788. doi: 10.1073/pnas.72.3.784. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ptashne M., Backman K., Humayun M. Z., Jeffrey A., Maurer R., Meyer B., Sauer R. T. Autoregulation and function of a repressor in bacteriophage lambda. Science. 1976 Oct 8;194(4261):156–161. doi: 10.1126/science.959843. [DOI] [PubMed] [Google Scholar]
  12. Reichardt L., Kaiser A. D. Control of lambda repressor synthesis. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2185–2189. doi: 10.1073/pnas.68.9.2185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Scherer G. Nucleotide sequence of the O gene and of the origin of replication in bacteriophage lambda DNA. Nucleic Acids Res. 1978 Sep;5(9):3141–3156. doi: 10.1093/nar/5.9.3141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shine J., Dalgarno L. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342–1346. doi: 10.1073/pnas.71.4.1342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Walz A., Pirrotta V., Ineichen K. Lambda repressor regulates the switch between PR and Prm promoters. Nature. 1976 Aug 19;262(5570):665–669. doi: 10.1038/262665a0. [DOI] [PubMed] [Google Scholar]
  16. Walz A., Pirrotta V. Sequence of the PR promoter of phage lambda. Nature. 1975 Mar 13;254(5496):118–121. doi: 10.1038/254118a0. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES