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. 1979;6(5):2003–2016. doi: 10.1093/nar/6.5.2003

Initiation of translation with Pseudomonas aeruginosa phage PP7 RNA: nucleotide sequence of the coat cistron ribosome binding site.

B A Bassel, D R Mills
PMCID: PMC327827  PMID: 109813

Abstract

Initiation complex formation between PP7 RNA and ribosomes of Pseudomonas aeruginosa and Escherichia coli has been investigated. The PP7 RNA fragments protected by both species of ribosome have been isolated, and their sequences have been determined. Only one binding sites is available on the intact PP7 RNA strand, and this site is recognized by ribosomes of both species. The PP7 RNA binding site is approximately 38 nucleotides long. It contains two AUG sequences and a purine-rich segment near the 5'-end that is complementary to segments near the 3'-ends of the 16S ribosomal RNA's of both P. aeruginosa and E. coli. In order to establish which of the AUG codons acts as the initiator, the H2N-terminal amino acid sequence of PP7 coat protein was determined. This sequence is compatible with the codon sequence following the second AUG codon. The extent of the reaction of PP7 RNA with E. coli ribosomes is greater than with P. aeruginosa ribosomes, but our results do not indicate a qualitative difference in the initial interaction between intact PP7 RNA and the ribosomes of either species.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bassel B. A., Curry M. E. Comparison of the activities of extracts of Escherichia coli and Salmonella typhimurium in amino acid incorporation. J Bacteriol. 1973 Nov;116(2):757–763. doi: 10.1128/jb.116.2.757-763.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bassel B. A., Walsh E. C., Chi J. Y., Curry M. E., Brown J. G. Translation of Pseudomonas aeruginosa bacteriophage PP7 RNA by a cell-free amino acid incorporating system from Escherichia coli. J Virol. 1974 Jun;13(6):1326–1330. doi: 10.1128/jvi.13.6.1326-1330.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bertrand K., Korn L., Lee F., Platt T., Squires C. L., Squires C., Yanofsky C. New features of the regulation of the tryptophan operon. Science. 1975 Jul 4;189(4196):22–26. doi: 10.1126/science.1094538. [DOI] [PubMed] [Google Scholar]
  4. Davies J. W., Benike C. Translation of virus mRNA: synthesis of bacteriophage PP7 proteins in cell-free extracts from Pseudomonas aeruginosa. Virology. 1974 Oct;61(2):450–457. doi: 10.1016/0042-6822(74)90281-5. [DOI] [PubMed] [Google Scholar]
  5. Gupta S. L., Chen J., Schaefer L., Lengyel P., Weissman S. M. Nucleotide sequence of a ribosome attachment site of bacteriophage f2 RNA. Biochem Biophys Res Commun. 1970 Jun 5;39(5):883–888. doi: 10.1016/0006-291x(70)90406-7. [DOI] [PubMed] [Google Scholar]
  6. Himes R. H., Stallcup M. R., Rabinowitz J. C. Translation of synthetic and endogenous messenger ribonucleic acid in vitro by ribosomes and polyribosomes from Clostridium pasteurianum. J Bacteriol. 1972 Dec;112(3):1057–1069. doi: 10.1128/jb.112.3.1057-1069.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Howk R., Sarimo S. S., Pine M. J. Translation of RNA coliphages by amino acid-incorporation systems of the Enterobacteriaceae. J Gen Microbiol. 1973 Jan;74(1):93–96. doi: 10.1099/00221287-74-1-93. [DOI] [PubMed] [Google Scholar]
  8. Leffler S., Szer W. Messenger selection by bacterial ribosomes. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2364–2368. doi: 10.1073/pnas.70.8.2364. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Leffler S., Szer W. Polypeptide chain initiation in Caulobacter crescentus without initiation factor IF-1. J Biol Chem. 1974 Mar 10;249(5):1465–1468. [PubMed] [Google Scholar]
  10. Lodish H. F., Robertson H. D. Regulation of in vitro translation of bacteriophage f2 RNA. Cold Spring Harb Symp Quant Biol. 1969;34:655–673. doi: 10.1101/sqb.1969.034.01.076. [DOI] [PubMed] [Google Scholar]
  11. Lodish H. F. Species specificity of polypeptide chain initiation. Nature. 1969 Nov 29;224(5222):867–870. doi: 10.1038/224867a0. [DOI] [PubMed] [Google Scholar]
  12. Lodish H. F. Specificity in bacterial protein synthesis: role of initiation factors and ribosomal subunits. Nature. 1970 May 23;226(5247):705–707. doi: 10.1038/226705a0. [DOI] [PubMed] [Google Scholar]
  13. Maizels N. E. coli lactose operon ribosome binding site. Nature. 1974 Jun 14;249(458):647–649. doi: 10.1038/249647b0. [DOI] [PubMed] [Google Scholar]
  14. Musso R. E., de Crombrugghe B., Pastan I., Sklar J., Yot P., Weissman S. The 5'-terminal nucleotide sequence of galactose messenger ribonucleic acid of Escherichia coli. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4940–4944. doi: 10.1073/pnas.71.12.4940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pieczenik G., Model P., Robertson H. D. Sequence and symmetry in ribosome binding sites of bacteriophage f1 RNA. J Mol Biol. 1974 Dec 5;90(2):191–124. doi: 10.1016/0022-2836(74)90368-4. [DOI] [PubMed] [Google Scholar]
  16. Platt T., Yanofsky C. An intercistronic region and ribosome-binding site in bacterial messenger RNA. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2399–2403. doi: 10.1073/pnas.72.6.2399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Robertson H. D., Barrell B. G., Weith H. L., Donelson J. E. Isolation and sequence analysis of a ribosome-protected fragment from bacteriophage phiX 174 DNA. Nat New Biol. 1973 Jan 10;241(106):38–40. doi: 10.1038/newbio241038a0. [DOI] [PubMed] [Google Scholar]
  18. Shine J., Dalgarno L. Determinant of cistron specificity in bacterial ribosomes. Nature. 1975 Mar 6;254(5495):34–38. doi: 10.1038/254034a0. [DOI] [PubMed] [Google Scholar]
  19. Stallcup M. R., Rabinowitz J. C. Initiation of protein synthesis in vitro by a clostridial system. I. Specificity in the translation of natural messenger ribonucleic acids. J Biol Chem. 1973 May 10;248(9):3209–3215. [PubMed] [Google Scholar]
  20. Stallcup M. R., Rabinowitz J. C. Initiation of protein synthesis in vitro by a clostridial system. II. The roles of initiation factors and salt-washed ribosomes in determining specificity in the translation of natural messenger ribonucleic acids. J Biol Chem. 1973 May 10;248(9):3216–3219. [PubMed] [Google Scholar]
  21. Staples D. H., Hindley J., Billeter M. A., Weissmann C. Localization of Q-beta maturation cistron ribosome binding site. Nat New Biol. 1971 Sep 15;234(50):202–204. doi: 10.1038/newbio234202a0. [DOI] [PubMed] [Google Scholar]
  22. Staples D. H., Hindley J. Ribosome binding site of Q-beta RNA polymerase cistron. Nat New Biol. 1971 Sep 15;234(50):211–212. doi: 10.1038/newbio234211a0. [DOI] [PubMed] [Google Scholar]
  23. Steege D. A. 5'-Terminal nucleotide sequence of Escherichia coli lactose repressor mRNA: features of translational initiation and reinitiation sites. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4163–4167. doi: 10.1073/pnas.74.10.4163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Steege D. A. A ribosome binding site from the pR RNA of bacteriophage lambda. J Mol Biol. 1977 Aug 25;114(4):559–568. doi: 10.1016/0022-2836(77)90178-4. [DOI] [PubMed] [Google Scholar]
  25. Steitz J. A., Bryan R. A. Two ribosome binding sites from the gene 0-3 messenger RNA of bacteriophages T7. J Mol Biol. 1977 Aug 25;114(4):527–543. doi: 10.1016/0022-2836(77)90176-0. [DOI] [PubMed] [Google Scholar]
  26. Steitz J. A. Oligonucleotide sequence of replicase initiation site in Q RNA. Nat New Biol. 1972 Mar 22;236(64):71–75. doi: 10.1038/newbio236071a0. [DOI] [PubMed] [Google Scholar]
  27. Steitz J. A. Polypeptide chain initiation: nucleotide sequences of the three ribosomal binding sites in bacteriophage R17 RNA. Nature. 1969 Dec 6;224(5223):957–964. doi: 10.1038/224957a0. [DOI] [PubMed] [Google Scholar]
  28. Szer W., Brenowitz J. Translation of MS2 RNA by ribosomes from different bacterial species. Biochem Biophys Res Commun. 1970 Mar 27;38(6):1154–1160. doi: 10.1016/0006-291x(70)90360-8. [DOI] [PubMed] [Google Scholar]
  29. Szer W., Leffler S. Interaction of Escherichia coli 30S ribosomal subunits with MS2 phage RNA in the absence of initiation factors. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3611–3615. doi: 10.1073/pnas.71.9.3611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Weiner A. M., Platt T., Weber K. Amino-terminal sequence analysis of proteins purified on a nanomole scale by gel electrophoresis. J Biol Chem. 1972 May 25;247(10):3242–3251. [PubMed] [Google Scholar]
  31. Woese C., Sogin M., Stahl D., Lewis B. J., Bonen L. A comparison of the 16S ribosomal RNAs from mesophilic and thermophilic bacilli: some modifications in the Sanger method for RNA sequencing. J Mol Evol. 1976 Apr 9;7(3):197–213. doi: 10.1007/BF01731489. [DOI] [PubMed] [Google Scholar]

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