Abstract
Attempts to prevent the urea conversion of a 200-230,000 molecular weight DNA polymerase alpha to a 150-170,000 molecular weight form by the inclusion of protease inhibitors have not been successful. No other method has been found capable of dissociating a 50-70,000 fragment or subunit from the DNA polymerase subunit. Addition of this 50-70,000 subunit to the polymerase subunit does not aid the binding of the enzyme to DNA, but does have an effect on the utilisation of synthetic template-initiator complexes by the polymerase subunit.
Full text
PDF











Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bollum F. J. Mammalian DNA polymerases. Prog Nucleic Acid Res Mol Biol. 1975;15(0):109–144. doi: 10.1016/s0079-6603(08)60118-x. [DOI] [PubMed] [Google Scholar]
- Brakel C. L., Blumenthal A. B. Multiple forms of Drosophila embryo DNA polymerase: evidence for proteolytic conversion. Biochemistry. 1977 Jul 12;16(14):3137–3143. doi: 10.1021/bi00633a016. [DOI] [PubMed] [Google Scholar]
- Chang L. M., Cassani G. R., Bollum F. J. Deoxynucleotide-polymerizing enzymes of calf thymus gland. VII. Replication of homopolymers. J Biol Chem. 1972 Dec 10;247(23):7718–7723. [PubMed] [Google Scholar]
- Craig R. K., Keir H. M. Deoxyribonucleic acid poymerase of BHK-21/C13 cells. Heterogeneity, molecular asymmetry and subcellular distribution of the enzymes. Biochem J. 1975 Feb;145(2):225–232. doi: 10.1042/bj1450225. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fisher P. A., Korn D. DNA polymerase-alpha. Purification and structural characterization of the near homogeneous enzyme from human KB cells. J Biol Chem. 1977 Sep 25;252(18):6528–6535. [PubMed] [Google Scholar]
- Hachmann H. J., Lezius A. G. High-molecular-weight DNA polymerases from mouse myeloma. Purification and properties of three enzymes. Eur J Biochem. 1975 Jan 2;50(2):357–366. doi: 10.1111/j.1432-1033.1975.tb09811.x. [DOI] [PubMed] [Google Scholar]
- Hesslewood I. P., Holmes A. M., Wakeling W. F., Johnston I. R. Studies on the purification and properties of a 6.8-S DNA polymerase activity found in calf-thymus DNA polymerase-alpha fraction. Eur J Biochem. 1978 Mar;84(1):123–131. doi: 10.1111/j.1432-1033.1978.tb12148.x. [DOI] [PubMed] [Google Scholar]
- Holmes A. M., Hesslewood I. P., Johnston I. R. Evidence that DNA polymerase-alpha of calf thymus contains a subunit of molecular weight 155000. Eur J Biochem. 1976 Feb 16;62(2):229–235. doi: 10.1111/j.1432-1033.1976.tb10152.x. [DOI] [PubMed] [Google Scholar]
- Holmes A. M., Hesslewood I. P., Johnston I. R. In vitro concersion of a calf thymus 8S DNA polymerase to a 7.3S species. Nature. 1975 May 29;255(5507):420–422. doi: 10.1038/255420a0. [DOI] [PubMed] [Google Scholar]
- Holmes A. M., Hesslewood I. P., Wickremasinghe R. G., Johnston I. R. The heterogeneity of deoxyribonucleic acid polymerase-alpha. Biochem Soc Symp. 1977;(42):17–36. [PubMed] [Google Scholar]
- Lowe P. A., Malcolm A. D. Structural properties of Escherichia coli RNA polymerase Subunits. Eur J Biochem. 1976 Apr 15;64(1):177–188. doi: 10.1111/j.1432-1033.1976.tb10286.x. [DOI] [PubMed] [Google Scholar]
- Matsukage A., Bohn E. W., Wilson S. H. Multiple forms of DNA polymerase in mouse myeloma. Proc Natl Acad Sci U S A. 1974 Feb;71(2):578–582. doi: 10.1073/pnas.71.2.578. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matsukage A., Sivarajan M., Wilson S. H. Studies on DNA alpha-polymerase of mouse myeloma: partial purification and comparison of three molecular forms of the enzyme. Biochemistry. 1976 Nov 30;15(24):5305–5314. doi: 10.1021/bi00669a017. [DOI] [PubMed] [Google Scholar]
- Momparler R. L., Rossi M., Labitan A. Partial purification and properties of two forms of deoxyribonucleic acid polymerase from calf thymus. J Biol Chem. 1973 Jan 10;248(1):285–293. [PubMed] [Google Scholar]
- Méchali M., de Recondo A. M. Detection of a DNA-binding factor associated with mammalian DNA polymerase-alpha. Biochem Biophys Res Commun. 1978 May 15;82(1):255–264. doi: 10.1016/0006-291x(78)90603-4. [DOI] [PubMed] [Google Scholar]
- Noy G. P., Weissbach A. HeLa cell DNA polymerases: the effect of cycloheximide in vivo and detection of a new form of DNA polymerase alpha. Biochim Biophys Acta. 1977 Jul 5;477(1):70–83. doi: 10.1016/0005-2787(77)90161-7. [DOI] [PubMed] [Google Scholar]
- RICHARDS F. M., VITHAYATHIL P. J. Peptide-protein interactions in ribonuclease-S. Brookhaven Symp Biol. 1960 Nov;13:115–134. [PubMed] [Google Scholar]
- Wickremasinghe R. G., Johnston I. R. Evidence that the DNA- and poly(A)-dependent DNA polymerase activities of rat liver nuclei are functions of the same enzyme. Some observations on its mode of action. Biochim Biophys Acta. 1974 Aug 15;361(1):37–52. doi: 10.1016/0005-2787(74)90207-x. [DOI] [PubMed] [Google Scholar]
- Yoshida S., Kondo T., Ando T. Multiple molecular species of cytoplasmic DNA polymerase from calf thymus. Biochim Biophys Acta. 1974 Jul 24;353(4):463–474. doi: 10.1016/0005-2787(74)90052-5. [DOI] [PubMed] [Google Scholar]
- de Jong A., van der Vliet P. C., Jansz H. S. DNA polymerases in adenovirus type 5-infected and uninfected KB cells. Induction of an alpha-type DNA polymerase in adenovirus type 5-infected and in fast growing cells. Biochim Biophys Acta. 1977 May 17;476(2):156–165. doi: 10.1016/0005-2787(77)90092-2. [DOI] [PubMed] [Google Scholar]