Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1979 Sep 25;7(2):361–375. doi: 10.1093/nar/7.2.361

Sequence of 1019 nucleotides encompassing one of the inverted repeats from the yeast 2 micrometer plasmid.

J Hindley, G A Phear
PMCID: PMC328023  PMID: 386282

Abstract

A sequence of 1019 nucleotides encompassing one of the 600 base inverted repeats and non-repeated flanking regions has been determined in the type A yeast 2 micrometers plasmid cloned in pMB9. Methods are described for applying the Maxam-Gilbert sequencing procedure to DNA fragments labelled at the 3'-end using a T4-polymerase exchange/repair reaction and for sequencing 5'-end labelled fragments using dideoxy-nucleotides as chain terminators in the presence of E. coli DNA polymerase (nach Klenow). A notable feature of the sequence is its unusual content of symmetry elements. In one region of 140 nucleotides, 137 are involved in a complex arrangement of direct and inverted repeats linked by palindromic sequences.

Full text

PDF
365

Images in this article

366Image
on p.366
367Image
on p.367
368Image
on p.368
370Image
on p.370

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beggs J. D., Guerineau M., Atkins J. F. A map of the restriction targets in yeast 2 micron plasmid DNA cloned on bacteriophage lambda. Mol Gen Genet. 1976 Nov 17;148(3):287–294. doi: 10.1007/BF00332903. [DOI] [PubMed] [Google Scholar]
  2. Beggs J. D. Transformation of yeast by a replicating hybrid plasmid. Nature. 1978 Sep 14;275(5676):104–109. doi: 10.1038/275104a0. [DOI] [PubMed] [Google Scholar]
  3. Broach J. R., Atkins J. F., McGill C., Chow L. Identification and mapping of the transcriptional and translational products of the yeast plasmid, 2mu circle. Cell. 1979 Apr;16(4):827–839. doi: 10.1016/0092-8674(79)90098-9. [DOI] [PubMed] [Google Scholar]
  4. Cameron J. R., Philippsen P., Davis R. W. Analysis of chromosomal integration and deletions of yeast plasmids. Nucleic Acids Res. 1977;4(5):1429–1448. doi: 10.1093/nar/4.5.1429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Clewell D. B., Helinski D. R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. doi: 10.1073/pnas.62.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fiddes J. C. Nucleotide sequence of the intercistronic region between genes G and F in bacteriophage phiX174 DNA. J Mol Biol. 1976 Oct 15;107(1):1–24. doi: 10.1016/s0022-2836(76)80014-9. [DOI] [PubMed] [Google Scholar]
  7. Gerbaud C., Fournier P., Blanc H., Aigle M., Heslot H., Guerineau M. High frequency of yeast transformation by plasmids carrying part or entire 2-micron yeast plasmid. Gene. 1979 Mar;5(3):233–253. doi: 10.1016/0378-1119(79)90080-5. [DOI] [PubMed] [Google Scholar]
  8. Guerineau M., Grandchamp C., Slonimski P. P. Circular DNA of a yeast episome with two inverted repeats: structural analysis by a restriction enzyme and electron microscopy. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3030–3034. doi: 10.1073/pnas.73.9.3030. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Guerineau M., Slonimski P. P., Avner P. R. Yeast episome: oligomycin resistance associated with a small covalently closed non-mitochondrial circular DNA. Biochem Biophys Res Commun. 1974 Nov 27;61(2):462–469. doi: 10.1016/0006-291x(74)90979-6. [DOI] [PubMed] [Google Scholar]
  10. Hollenberg C. P., Degelmann A., Kustermann-Kuhn B., Royer H. D. Characterization of 2-mum DNA of Saccharomyces cerevisiae by restriction fragment analysis and integration in an Escherichia coli plasmid. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2072–2076. doi: 10.1073/pnas.73.6.2072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kleckner N. Translocatable elements in procaryotes. Cell. 1977 May;11(1):11–23. doi: 10.1016/0092-8674(77)90313-0. [DOI] [PubMed] [Google Scholar]
  12. Livingston D. M., Klein H. L. Deoxyribonucleic acid sequence organization of a yeast plasmid. J Bacteriol. 1977 Jan;129(1):472–481. doi: 10.1128/jb.129.1.472-481.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Meijer M., Beck E., Hansen F. G., Bergmans H. E., Messer W., von Meyenburg K., Schaller H. Nucleotide sequence of the origin of replication of the Escherichia coli K-12 chromosome. Proc Natl Acad Sci U S A. 1979 Feb;76(2):580–584. doi: 10.1073/pnas.76.2.580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sanger F., Coulson A. R. The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978 Mar 1;87(1):107–110. doi: 10.1016/0014-5793(78)80145-8. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES