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. 1991 Jun 25;19(12):3315–3318. doi: 10.1093/nar/19.12.3315

Mapping irradiation hybrids to cosmid and yeast artificial chromosome libraries by direct hybridization of Alu-PCR products.

A P Monaco 1, V M Lam 1, G Zehetner 1, G G Lennon 1, C Douglas 1, D Nizetic 1, P N Goodfellow 1, H Lehrach 1
PMCID: PMC328328  PMID: 2062647

Abstract

A direct hybridization protocol is described for screening cosmid and yeast artificial chromosome libraries with pools of Alu-PCR products from somatic cell or irradiation hybrids. This method eliminates purification, cloning and analysis of each individual Alu-PCR product before library screening. A series of human X chromosome irradiation hybrids were mapped by this method, using a cosmid reference library for comparisons between overlapping hybrids to identify interesting clones for further analysis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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