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. 1991 Jun 25;19(12):3325–3330. doi: 10.1093/nar/19.12.3325

A new allele of the duplicated 27kD zein locus of maize generated by homologous recombination.

O P Das 1, E Poliak 1, K Ward 1, J Messing 1
PMCID: PMC328330  PMID: 2062649

Abstract

The 27kD zein storage protein locus in many inbred lines of maize consists of a tandem duplication of 12kb, with an expressed gene in each repeat, termed A and B. A single-copy allele with only the A gene can be generated from this duplication in particular stocks of the maize inbred line A188 by a mitotic process that includes a crossover at the 3' regions of the two genes (1). Here, we characterize a second single-copy allele with only the B gene, found in different stocks of A188. This allele arises from a homologous recombination at the highly conserved 5' regions of the two repeats, and cloning and sequencing of this allele define the crossover region. The A and B genes in the duplicated allele were previously shown to be expressed at different levels; this difference remains unchanged in either recombinant allele. Therefore, the crossover points of these two recombinant alleles define the borders of cis-acting sequences responsible for the differential expression of the two genes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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