Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1991 Jun 25;19(12):3325–3330. doi: 10.1093/nar/19.12.3325

A new allele of the duplicated 27kD zein locus of maize generated by homologous recombination.

O P Das 1, E Poliak 1, K Ward 1, J Messing 1
PMCID: PMC328330  PMID: 2062649

Abstract

The 27kD zein storage protein locus in many inbred lines of maize consists of a tandem duplication of 12kb, with an expressed gene in each repeat, termed A and B. A single-copy allele with only the A gene can be generated from this duplication in particular stocks of the maize inbred line A188 by a mitotic process that includes a crossover at the 3' regions of the two genes (1). Here, we characterize a second single-copy allele with only the B gene, found in different stocks of A188. This allele arises from a homologous recombination at the highly conserved 5' regions of the two repeats, and cloning and sequencing of this allele define the crossover region. The A and B genes in the duplicated allele were previously shown to be expressed at different levels; this difference remains unchanged in either recombinant allele. Therefore, the crossover points of these two recombinant alleles define the borders of cis-acting sequences responsible for the differential expression of the two genes.

Full text

PDF
3327

Images in this article

3326Image
on p.3326
3327Image
on p.3327
3327Image
on p.3327

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brawerman G. Determinants of messenger RNA stability. Cell. 1987 Jan 16;48(1):5–6. doi: 10.1016/0092-8674(87)90346-1. [DOI] [PubMed] [Google Scholar]
  2. Chodchoy N., Levine B. J., Sprecher C., Skoultchi A. I., Marzluff W. F. Expression of mouse histone genes: transcription into 3' intergenic DNA and cryptic processing sites downstream from the 3' end of the H3 gene. Mol Cell Biol. 1987 Mar;7(3):1039–1047. doi: 10.1128/mcb.7.3.1039. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chomet P. S., Wessler S., Dellaporta S. L. Inactivation of the maize transposable element Activator (Ac) is associated with its DNA modification. EMBO J. 1987 Feb;6(2):295–302. doi: 10.1002/j.1460-2075.1987.tb04753.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Das O. P., Levi-Minzi S., Koury M., Benner M., Messing J. A somatic gene rearrangement contributing to genetic diversity in maize. Proc Natl Acad Sci U S A. 1990 Oct;87(20):7809–7813. doi: 10.1073/pnas.87.20.7809. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Das O. P., Messing J. W. Allelic variation and differential expression at the 27-kilodalton zein locus in maize. Mol Cell Biol. 1987 Dec;7(12):4490–4497. doi: 10.1128/mcb.7.12.4490. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dooner H. K., Kermicle J. L. Structure of the R tandem duplication in maize. Genetics. 1971 Mar;67(3):427–436. doi: 10.1093/genetics/67.3.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kirihara J. A., Petri J. B., Messing J. Isolation and sequence of a gene encoding a methionine-rich 10-kDa zein protein from maize. Gene. 1988 Nov 30;71(2):359–370. doi: 10.1016/0378-1119(88)90053-4. [DOI] [PubMed] [Google Scholar]
  8. Maeda N., Smithies O. The evolution of multigene families: human haptoglobin genes. Annu Rev Genet. 1986;20:81–108. doi: 10.1146/annurev.ge.20.120186.000501. [DOI] [PubMed] [Google Scholar]
  9. McStay B., Reeder R. H. A termination site for Xenopus RNA polymerase I also acts as an element of an adjacent promoter. Cell. 1986 Dec 26;47(6):913–920. doi: 10.1016/0092-8674(86)90806-8. [DOI] [PubMed] [Google Scholar]
  10. Pedersen K., Argos P., Naravana S. V., Larkins B. A. Sequence analysis and characterization of a maize gene encoding a high-sulfur zein protein of Mr 15,000. J Biol Chem. 1986 May 15;261(14):6279–6284. [PubMed] [Google Scholar]
  11. Peterhans A., Schlüpmann H., Basse C., Paszkowski J. Intrachromosomal recombination in plants. EMBO J. 1990 Nov;9(11):3437–3445. doi: 10.1002/j.1460-2075.1990.tb07551.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Peterson T. Intragenic transposition of Ac generates a new allele of the maize P gene. Genetics. 1990 Oct;126(2):469–476. doi: 10.1093/genetics/126.2.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wang S. Z., Esen A. Primary structure of a proline-rich zein and its cDNA. Plant Physiol. 1986 May;81(1):70–74. doi: 10.1104/pp.81.1.70. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wilson C. M. Serial analysis of zein by isoelectric focusing and sodium dodecyl sulfate gel electrophoresis. Plant Physiol. 1986 Sep;82(1):196–202. doi: 10.1104/pp.82.1.196. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES