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. 1991 Sep 11;19(17):4623–4629. doi: 10.1093/nar/19.17.4623

Removal of psoralen monoadducts and crosslinks by human cell free extracts.

J T Reardon 1, P Spielmann 1, J C Huang 1, S Sastry 1, A Sancar 1, J E Hearst 1
PMCID: PMC328701  PMID: 1891353

Abstract

Human cell free extracts are capable of carrying out damage-induced DNA synthesis in response to DNA damage by UV, psoralen, and cisplatin. We show that this damage-induced DNA synthesis is associated with removal of psoralen adducts and therefore is 'repair synthesis' and not an aberrant DNA synthesis reaction potentiated by DNA deformed by adducts. By comparing the denaturable fraction of psoralen adducted DNA which becomes labeled in the repair reaction to that of terminally labeled DNA (without repair) we have found that all DNA synthesis induced by psoralen monoadducts is the consequence of removal of these adducts. By the same approach we have obtained preliminary evidence that this in vitro system is capable of removing psoralen crosslinks as well.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chatterjee P. K., Cantor C. R. Photochemical production of psoralen - DNA monoadducts capable of subsequent photocrosslinking. Nucleic Acids Res. 1978 Oct;5(10):3619–3633. doi: 10.1093/nar/5.10.3619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cheng S., Sancar A., Hearst J. E. RecA-dependent incision of psoralen-crosslinked DNA by (A)BC excinuclease. Nucleic Acids Res. 1991 Feb 11;19(3):657–663. doi: 10.1093/nar/19.3.657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cheng S., Van Houten B., Gamper H. B., Sancar A., Hearst J. E. Use of psoralen-modified oligonucleotides to trap three-stranded RecA-DNA complexes and repair of these cross-linked complexes by ABC excinuclease. J Biol Chem. 1988 Oct 15;263(29):15110–15117. [PubMed] [Google Scholar]
  4. Cimino G. D., Shi Y. B., Hearst J. E. Wavelength dependence for the photoreversal of a psoralen-DNA cross-link. Biochemistry. 1986 May 20;25(10):3013–3020. doi: 10.1021/bi00358a042. [DOI] [PubMed] [Google Scholar]
  5. Coverley D., Kenny M. K., Munn M., Rupp W. D., Lane D. P., Wood R. D. Requirement for the replication protein SSB in human DNA excision repair. Nature. 1991 Feb 7;349(6309):538–541. doi: 10.1038/349538a0. [DOI] [PubMed] [Google Scholar]
  6. Hansson J., Munn M., Rupp W. D., Kahn R., Wood R. D. Localization of DNA repair synthesis by human cell extracts to a short region at the site of a lesion. J Biol Chem. 1989 Dec 25;264(36):21788–21792. [PubMed] [Google Scholar]
  7. Hansson J., Wood R. D. Repair synthesis by human cell extracts in DNA damaged by cis- and trans-diamminedichloroplatinum(II). Nucleic Acids Res. 1989 Oct 25;17(20):8073–8091. doi: 10.1093/nar/17.20.8073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Heiger-Bernays W. J., Essigmann J. M., Lippard S. J. Effect of the antitumor drug cis-diamminedichloroplatinum(II) and related platinum complexes on eukaryotic DNA replication. Biochemistry. 1990 Sep 11;29(36):8461–8466. doi: 10.1021/bi00488a037. [DOI] [PubMed] [Google Scholar]
  9. Kanne D., Straub K., Rapoport H., Hearst J. E. Psoralen-deoxyribonucleic acid photoreaction. Characterization of the monoaddition products from 8-methoxypsoralen and 4,5'8-trimethylpsoralen. Biochemistry. 1982 Mar 2;21(5):861–871. doi: 10.1021/bi00534a008. [DOI] [PubMed] [Google Scholar]
  10. Koo H. S., Claassen L., Grossman L., Liu L. F. ATP-dependent partitioning of the DNA template into supercoiled domains by Escherichia coli UvrAB. Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1212–1216. doi: 10.1073/pnas.88.4.1212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Manley J. L., Fire A., Cano A., Sharp P. A., Gefter M. L. DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3855–3859. doi: 10.1073/pnas.77.7.3855. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sancar A., Sancar G. B. DNA repair enzymes. Annu Rev Biochem. 1988;57:29–67. doi: 10.1146/annurev.bi.57.070188.000333. [DOI] [PubMed] [Google Scholar]
  13. Sibghat-Ullah, Sancar A. Substrate overlap and functional competition between human nucleotide excision repair and Escherichia coli photolyase and (a)BC excision nuclease. Biochemistry. 1990 Jun 19;29(24):5711–5718. doi: 10.1021/bi00476a011. [DOI] [PubMed] [Google Scholar]
  14. Sibghatullah, Husain I., Carlton W., Sancar A. Human nucleotide excision repair in vitro: repair of pyrimidine dimers, psoralen and cisplatin adducts by HeLa cell-free extract. Nucleic Acids Res. 1989 Jun 26;17(12):4471–4484. doi: 10.1093/nar/17.12.4471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sladek F. M., Munn M. M., Rupp W. D., Howard-Flanders P. In vitro repair of psoralen-DNA cross-links by RecA, UvrABC, and the 5'-exonuclease of DNA polymerase I. J Biol Chem. 1989 Apr 25;264(12):6755–6765. [PubMed] [Google Scholar]
  16. Tessman J. W., Isaacs S. T., Hearst J. E. Photochemistry of the furan-side 8-methoxypsoralen-thymidine monoadduct inside the DNA helix. Conversion to diadduct and to pyrone-side monoadduct. Biochemistry. 1985 Mar 26;24(7):1669–1676. doi: 10.1021/bi00328a015. [DOI] [PubMed] [Google Scholar]
  17. Thomas D. C., Levy M., Sancar A. Amplification and purification of UvrA, UvrB, and UvrC proteins of Escherichia coli. J Biol Chem. 1985 Aug 15;260(17):9875–9883. [PubMed] [Google Scholar]
  18. Van Houten B., Gamper H., Holbrook S. R., Hearst J. E., Sancar A. Action mechanism of ABC excision nuclease on a DNA substrate containing a psoralen crosslink at a defined position. Proc Natl Acad Sci U S A. 1986 Nov;83(21):8077–8081. doi: 10.1073/pnas.83.21.8077. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Vos J. M., Hanawalt P. C. Processing of psoralen adducts in an active human gene: repair and replication of DNA containing monoadducts and interstrand cross-links. Cell. 1987 Aug 28;50(5):789–799. doi: 10.1016/0092-8674(87)90337-0. [DOI] [PubMed] [Google Scholar]
  20. Wood R. D. Repair of pyrimidine dimer ultraviolet light photoproducts by human cell extracts. Biochemistry. 1989 Oct 17;28(21):8287–8292. doi: 10.1021/bi00447a005. [DOI] [PubMed] [Google Scholar]
  21. Wood R. D., Robins P., Lindahl T. Complementation of the xeroderma pigmentosum DNA repair defect in cell-free extracts. Cell. 1988 Apr 8;53(1):97–106. doi: 10.1016/0092-8674(88)90491-6. [DOI] [PubMed] [Google Scholar]

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