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. 1990 May 25;18(10):2887–2890. doi: 10.1093/nar/18.10.2887

A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones.

J Riley 1, R Butler 1, D Ogilvie 1, R Finniear 1, D Jenner 1, S Powell 1, R Anand 1, J C Smith 1, A F Markham 1
PMCID: PMC330815  PMID: 2161516

Abstract

The recent development of yeast artificial chromosome (YAC) vectors has provided a system for cloning fragments that are over ten times larger than those that can be cloned in more established systems. We have developed a method for the rapid isolation of terminal sequences from YAC clones. The YAC clone is digested with a range of restriction enzymes, a common linker is ligated to the DNA fragments and terminal sequences are amplified using a vector specific primer and a linker specific primer. Sequence data derived from these terminal specific products can be used to design primers for a further round of screening to isolate overlapping clones. The method also provides a convenient method of generating Sequence Tagged Sites for the mapping of complex genomes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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