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. 1990 Oct 11;18(19):5673–5676. doi: 10.1093/nar/18.19.5673

Rat DNA polymerase beta gene can join in excision repair of Escherichia coli.

T Ohnishi 1, S Yuba 1, T Date 1, H Utsumi 1, A Matsukage 1
PMCID: PMC332299  PMID: 2216761

Abstract

Though DNA polymerase I (poll) of Escherichia (E.) coli is understood to play a role in repair synthesis of excision repair, it is still obscure whether DNA polymerase beta (pol beta) plays a similar role in eukaryotic cells. To estimate the role of pol beta in excision repair processes, we inserted the rat pol beta gene into several mutant E. coli defective in a diverse set of enzymatic activities of poll. UV resistance was seen only when the 5'----3' exonuclease (exo) activity of poll molecules remained. Therefore it is suggested that 5'----3' exo activity as well as pol beta activity are essential for repair synthesis of excision repair in eukaryotic cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abbotts J., SenGupta D. N., Zmudzka B., Widen S. G., Notario V., Wilson S. H. Expression of human DNA polymerase beta in Escherichia coli and characterization of the recombinant enzyme. Biochemistry. 1988 Feb 9;27(3):901–909. doi: 10.1021/bi00403a010. [DOI] [PubMed] [Google Scholar]
  2. Chang L. M. Phylogeny of DNA polymerase-beta. Science. 1976 Mar 19;191(4232):1183–1185. doi: 10.1126/science.769158. [DOI] [PubMed] [Google Scholar]
  3. Cleaver J. E. Structure of repaired sites in human DNA synthesized in the presence of inhibitors of DNA polymerases alpha and beta in human fibroblasts. Biochim Biophys Acta. 1983 Apr 15;739(3):301–311. doi: 10.1016/0167-4781(83)90105-7. [DOI] [PubMed] [Google Scholar]
  4. Date T. Demonstration by a novel genetic technique that leader peptidase is an essential enzyme of Escherichia coli. J Bacteriol. 1983 Apr;154(1):76–83. doi: 10.1128/jb.154.1.76-83.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Date T., Yamaguchi M., Hirose F., Nishimoto Y., Tanihara K., Matsukage A. Expression of active rat DNA polymerase beta in Escherichia coli. Biochemistry. 1988 Apr 19;27(8):2983–2990. doi: 10.1021/bi00408a048. [DOI] [PubMed] [Google Scholar]
  6. Fornace A. J., Jr, Zmudzka B., Hollander M. C., Wilson S. H. Induction of beta-polymerase mRNA by DNA-damaging agents in Chinese hamster ovary cells. Mol Cell Biol. 1989 Feb;9(2):851–853. doi: 10.1128/mcb.9.2.851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Friedberg E. C., Lehman I. R. Excision of thymine dimers by proteolytic and amber fragments of E. coli DNA polymerase I. Biochem Biophys Res Commun. 1974 May 7;58(1):132–139. doi: 10.1016/0006-291x(74)90901-2. [DOI] [PubMed] [Google Scholar]
  8. Glickman B. W. The role of DNA polymerase I in excision-repair. Basic Life Sci. 1975;5A:213–218. doi: 10.1007/978-1-4684-2895-7_28. [DOI] [PubMed] [Google Scholar]
  9. Grossman L., Braun A., Feldberg R., Mahler I. Enzymatic repair of DNA. Annu Rev Biochem. 1975;44:19–43. doi: 10.1146/annurev.bi.44.070175.000315. [DOI] [PubMed] [Google Scholar]
  10. Hanawalt P. C., Cooper P. K., Smith C. A. Repair replication schemes in bacteria and human cells. Prog Nucleic Acid Res Mol Biol. 1981;26:181–196. doi: 10.1016/s0079-6603(08)60404-3. [DOI] [PubMed] [Google Scholar]
  11. Heijneker H. L., Ellens D. J., Tjeerde R. H., Glickman B. W., van Dorp B., Pouwels P. H. A mutant of Escherichia coli K12 deficient in the 5'-3' exonucleolytic activity of DNA polymerase I. II. Purification and properties of the mutant enzyme. Mol Gen Genet. 1973 Jul 31;124(1):83–96. doi: 10.1007/BF00267167. [DOI] [PubMed] [Google Scholar]
  12. Kato T. Excision repair characteristics of recB - res - and uvrC - strains of Escherichia coli. J Bacteriol. 1972 Dec;112(3):1237–1246. doi: 10.1128/jb.112.3.1237-1246.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  14. Lehman I. R., Chien J. R. Persistence of deoxyribonucleic acid polymerase I and its 5'--3' exonuclease activity in PolA mutants of Escherichia coli K12. J Biol Chem. 1973 Nov 25;248(22):7717–7723. [PubMed] [Google Scholar]
  15. Lindell T. J., Weinberg F., Morris P. W., Roeder R. G., Rutter W. J. Specific inhibition of nuclear RNA polymerase II by alpha-amanitin. Science. 1970 Oct 23;170(3956):447–449. doi: 10.1126/science.170.3956.447. [DOI] [PubMed] [Google Scholar]
  16. Miller M. R., Chinault D. N. Evidence that DNA polymerases alpha and beta participate differentially in DNA repair synthesis induced by different agents. J Biol Chem. 1982 Jan 10;257(1):46–49. [PubMed] [Google Scholar]
  17. Mosbaugh D. W., Linn S. Excision repair and DNA synthesis with a combination of HeLa DNA polymerase beta and DNase V. J Biol Chem. 1983 Jan 10;258(1):108–118. [PubMed] [Google Scholar]
  18. Mosbaugh D. W., Meyer R. R. Interaction of mammalian deoxyribonuclease V, a double strand 3' to 5' and 5' to 3' exonuclease, with deoxyribonucleic acid polymerase-beta from the Novikoff hepatoma. J Biol Chem. 1980 Nov 10;255(21):10239–10247. [PubMed] [Google Scholar]
  19. Sancar A., Rupp W. D. A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides of the damaged region. Cell. 1983 May;33(1):249–260. doi: 10.1016/0092-8674(83)90354-9. [DOI] [PubMed] [Google Scholar]
  20. Sato N., Ohnishi T., Tano K., Yamamoto K., Nozu K. Induction of umuC+ gene expression in Escherichia coli irradiated by near ultraviolet light. Photochem Photobiol. 1985 Aug;42(2):135–139. doi: 10.1111/j.1751-1097.1985.tb01551.x. [DOI] [PubMed] [Google Scholar]
  21. Zmudzka B. Z., SenGupta D., Matsukage A., Cobianchi F., Kumar P., Wilson S. H. Structure of rat DNA polymerase beta revealed by partial amino acid sequencing and cDNA cloning. Proc Natl Acad Sci U S A. 1986 Jul;83(14):5106–5110. doi: 10.1073/pnas.83.14.5106. [DOI] [PMC free article] [PubMed] [Google Scholar]

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