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. 1990 Oct 25;18(20):6025–6029. doi: 10.1093/nar/18.20.6025

Thiophosphate interference experiments locate phosphates important for the hammerhead RNA self-cleavage reaction.

D E Ruffner 1, O C Uhlenbeck 1
PMCID: PMC332400  PMID: 2235484

Abstract

A hammerhead domain of less than 50 nucleotides is responsible for a self-cleavage reaction in the replication of plant RNA pathogens. The hammerhead is composed of three helices joining at a central conserved core of 11 single stranded nucleotides. The core is believed to fold into a tertiary structure that provides functional groups for catalysis and to coordinate one or more divalent metal ions. In this study we use a phosphorothioate substitution interference assay to identify four phosphates in the conserved core which also play a role in the self-cleavage reaction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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