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. 1990 Nov 25;18(22):6491–6495. doi: 10.1093/nar/18.22.6491

Solid-phase synthesis of H-Phe-Tyr-(pATAT)-NH2: a nucleopeptide fragment from the nucleoprotein of bacteriophage phi X174.

C M Dreef-Tromp 1, E M van Dam 1, H van den Elst 1, G A van der Marel 1, J H van Boom 1
PMCID: PMC332600  PMID: 2147473

Abstract

The preparation of the nucleopeptide H-Phe-Tyr-(pATAT)-NH2 could be realized via a solid phase phosphitetriester approach and by using the protected protecting group 2-(tert-butyldiphenylsilyoxymethyl)-benzoyl for the masking of the N6-amino function of deoxyadenosine. The latter protecting group can be removed under mild conditions with fluoride ion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ambros V., Baltimore D. Protein is linked to the 5' end of poliovirus RNA by a phosphodiester linkage to tyrosine. J Biol Chem. 1978 Aug 10;253(15):5263–5266. [PubMed] [Google Scholar]
  2. Baas P. D., Liewerink H., van Teeffelen H. A., van Mansfeld A. D., van Boom J. H., Jansz H. S. Alteration of the ATG start codon of the A protein of bacteriophage phi X174 into an ATT codon yields a viable phage indicating that A protein is not essential for phi X174 reproduction. FEBS Lett. 1987 Jun 22;218(1):119–125. doi: 10.1016/0014-5793(87)81030-x. [DOI] [PubMed] [Google Scholar]
  3. Bamford D. H., Mindich L. Characterization of the DNA-protein complex at the termini of the bacteriophage PRD1 genome. J Virol. 1984 May;50(2):309–315. doi: 10.1128/jvi.50.2.309-315.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chow M., Yabrov R., Bittle J., Hogle J., Baltimore D. Synthetic peptides from four separate regions of the poliovirus type 1 capsid protein VP1 induce neutralizing antibodies. Proc Natl Acad Sci U S A. 1985 Feb;82(3):910–914. doi: 10.1073/pnas.82.3.910. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hermoso J. M., Méndez E., Soriano F., Salas M. Location of the serine residue involved in the linkage between the terminal protein and the DNA of phage phi 29. Nucleic Acids Res. 1985 Nov 11;13(21):7715–7728. doi: 10.1093/nar/13.21.7715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kitamura N., Semler B. L., Rothberg P. G., Larsen G. R., Adler C. J., Dorner A. J., Emini E. A., Hanecak R., Lee J. J., van der Werf S. Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature. 1981 Jun 18;291(5816):547–553. doi: 10.1038/291547a0. [DOI] [PubMed] [Google Scholar]
  7. Kuyl-Yeheskiely E., Dreef-Tromp C. M., Geluk A., van der Marel G. A., van Boom J. H. Synthesis of the nucleopeptides H-Phe-Tyr(pGC)-NH2 and H-Phe-Ser(pGC)-Ala-OH via a phosphotriester approach. Nucleic Acids Res. 1989 Apr 25;17(8):2897–2905. doi: 10.1093/nar/17.8.2897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sinha N. D., Biernat J., McManus J., Köster H. Polymer support oligonucleotide synthesis XVIII: use of beta-cyanoethyl-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product. Nucleic Acids Res. 1984 Jun 11;12(11):4539–4557. doi: 10.1093/nar/12.11.4539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. van Mansfeld A. D., van Teeffelen H. A., Zandberg J., Baas P. D., Jansz H. S., Veeneman G. H., van Boom J. H. A protein of bacteriophage phi X174 carries an oligonucleotide which it can transfer to the 3' -OH of a DNA chain. FEBS Lett. 1982 Dec 13;150(1):103–108. doi: 10.1016/0014-5793(82)81313-6. [DOI] [PubMed] [Google Scholar]

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