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. 1992 Sep 25;20(18):4747–4751. doi: 10.1093/nar/20.18.4747

Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants.

P Schmieder 1, J H Ippel 1, H van den Elst 1, G A van der Marel 1, J H van Boom 1, C Altona 1, H Kessler 1
PMCID: PMC334227  PMID: 1408787

Abstract

Two heteronuclear proton-carbon NMR experiments are applied to the DNA-octamer d(TTGGCCAA)2 with carbon in natural abundance. They lead to a complete assignment of the carbon resonances of the sugars and bases. In addition, several heteronuclear coupling constants, proton-carbon as well as proton-phosphorous and phosphorous-carbon, were determined. The information can be obtained in a reasonable measuring time and offers valuable information for a detailed picture of DNA structure.

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Selected References

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