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. 1992 Oct 11;20(19):4981–4985. doi: 10.1093/nar/20.19.4981

Alw26I, Eco31I and Esp3I--type IIs methyltransferases modifying cytosine and adenine in complementary strands of the target DNA.

J Bitinaite 1, Z Maneliene 1, S Menkevicius 1, S Klimasauskas 1, V Butkus 1, A Janulaitis 1
PMCID: PMC334273  PMID: 1408816

Abstract

The specificity of three DNA methyltransferases M.Alw26I, M.Eco31I and M.Esp3I, isolated from Acinetobacter Iwoffi RFL26, Escherichia coli RFL31 and Hafnia alvei RFL3+, respectively, was determined. All the enzymes methylate both strands of asymmetric recognition sites yielding m5C in the top-strand and m6A in the bottom-strand, as below: 5'-GTm5CTC 5'-GGTm5CTC 5'-CGTm5CTC 3'-Cm6AGAG 3'-CCm6AGAG 3'-GCm6AGAG (M.Alw26I) (M.Eco31I) (M.Esp3I) They are the first members of type IIs methyltransferases that modify different types of nucleotides in the recognition sequence.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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