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. 1989 Oct 25;17(20):8185–8195. doi: 10.1093/nar/17.20.8185

Binding of a pancreatic nuclear protein is correlated with amylase enhancer activity.

G Howard 1, P R Keller 1, T M Johnson 1, M H Meisler 1
PMCID: PMC334957  PMID: 2478959

Abstract

The mouse amylase gene Amy-2.2 is expressed at high levels specifically in the acinar cells of the pancreas. The region between -172 and -110 of this gene includes sequence elements common to pancreas-specific genes. Nuclear proteins with specific affinity for this region were partially purified from rat pancreas. The consensus element of another pancreas-specific gene, elastase 1, competes for protein binding to the amylase sequences. Binding was localized by DNase I protection to the sequence -156 to -122. Site-directed mutagenesis of this sequence resulted in concomitant loss of protein binding and enhancer activity. Photo-affinity labelling of pancreatic nuclear extracts identified one predominant binding protein with a molecular weight of approximately 75 kDa. The data indicate that binding of this nuclear protein is essential for the enhancer activity of this pancreas-specific element.

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