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. 1989 Dec 11;17(23):9649–9660. doi: 10.1093/nar/17.23.9649

The use of 5'-phospho-2 deoxyribocytidylylriboadenosine as a facile route to chemical aminoacylation of tRNA.

S A Robertson 1, C J Noren 1, S J Anthony-Cahill 1, M C Griffith 1, P G Schultz 1
PMCID: PMC335204  PMID: 2602139

Abstract

Methodology is described for the synthesis and chemical aminoacylation of the hybrid dinucleotide 5'-phospho-2'-deoxyribocytidylylriboadenosine (pdCpA). Ligation of aminoacylated pdCpA to a truncated amber suppressor tRNACUA (-CA) using T4 RNA ligase generates an aminoacylated suppressor tRNA which can be used for site-specific incorporation of unnatural amino acids into proteins. Both the ligation and in vitro suppression efficiencies are the same when either pCpA or pdCpA is used. The use of deoxycytidine simplifies the chemistry involved in the synthesis of the dinucleotide pCpA. In addition, these results demonstrate that ribocytidine is not required for recognition of the aminoacylated tRNA during protein synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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