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. 1989 Dec 11;17(23):9947–9956. doi: 10.1093/nar/17.23.9947

Sequence limits of DNA strands in the arrest replication fork at the Bacillus subtilis chromosome terminus.

N K Williams 1, R G Wake 1
PMCID: PMC335224  PMID: 2513558

Abstract

The DNA sequence limits of the leading and lagging strands in the arrested clockwise replication fork at the terminus of the Bacillus subtilis chromosome have been investigated. On the basis of hybridization to synthetic oligonucleotides corresponding to known positions in the terminus region sequence it has been shown that neither the leading nor lagging strands, as they approach terC, traverse the distal inverted repeat, IRI. But a small fraction of the leading strands pass through the proximal inverted repeat, IRII. This is consistent with IRI being the functional inverted repeat in arresting the clockwise fork. But most of the forks appear to stop at least 100 nucleotides short of IRI, and at various positions extending over a distance of at least 100 nucleotides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bastia D., Germino J., Crosa J. H., Ram J. The nucleotide sequence surrounding the replication terminus of R6K. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2095–2099. doi: 10.1073/pnas.78.4.2095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carrigan C. M., Haarsma J. A., Smith M. T., Wake R. G. Sequence features of the replication terminus of the Bacillus subtilis chromosome. Nucleic Acids Res. 1987 Oct 26;15(20):8501–8509. doi: 10.1093/nar/15.20.8501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hanley P. J., Carrigan C. M., Rowe D. B., Wake R. G. Breakdown and quantitation of the forked termination of replication intermediate of Bacillus subtilis. J Mol Biol. 1987 Aug 5;196(3):721–727. doi: 10.1016/0022-2836(87)90043-x. [DOI] [PubMed] [Google Scholar]
  4. Hill T. M., Pelletier A. J., Tecklenburg M. L., Kuempel P. L. Identification of the DNA sequence from the E. coli terminus region that halts replication forks. Cell. 1988 Nov 4;55(3):459–466. doi: 10.1016/0092-8674(88)90032-3. [DOI] [PubMed] [Google Scholar]
  5. Horiuchi T., Hidaka M. Core sequence of two separable terminus sites of the R6K plasmid that exhibit polar inhibition of replication is a 20 bp inverted repeat. Cell. 1988 Aug 12;54(4):515–523. doi: 10.1016/0092-8674(88)90073-6. [DOI] [PubMed] [Google Scholar]
  6. Kolter R., Helinski D. R. Activity of the replication terminus of plasmid R6K in hybrid replicons in Escherichia coli. J Mol Biol. 1978 Sep 25;124(3):425–441. doi: 10.1016/0022-2836(78)90180-8. [DOI] [PubMed] [Google Scholar]
  7. Lewis P. J., Smith M. T., Wake R. G. A protein involved in termination of chromosome replication in Bacillus subtilis binds specifically to the terC site. J Bacteriol. 1989 Jun;171(6):3564–3567. doi: 10.1128/jb.171.6.3564-3567.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sista P. R., Mukherjee S., Patel P., Khatri G. S., Bastia D. A host-encoded DNA-binding protein promotes termination of plasmid replication at a sequence-specific replication terminus. Proc Natl Acad Sci U S A. 1989 May;86(9):3026–3030. doi: 10.1073/pnas.86.9.3026. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Smith M. T., Wake R. G. DNA sequence requirements for replication fork arrest at terC in Bacillus subtilis. J Bacteriol. 1988 Sep;170(9):4083–4090. doi: 10.1128/jb.170.9.4083-4090.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Weiss A. S., Wake R. G. A unique DNA intermediate associated with termination of chromosome replication in Bacillus subtilis. Cell. 1984 Dec;39(3 Pt 2):683–689. doi: 10.1016/0092-8674(84)90475-6. [DOI] [PubMed] [Google Scholar]
  11. Weiss A. S., Wake R. G., Inman R. B. An immobilized fork as a termination of replication intermediate in Bacillus subtilis. J Mol Biol. 1986 Mar 20;188(2):199–205. doi: 10.1016/0022-2836(86)90304-9. [DOI] [PubMed] [Google Scholar]
  12. Weiss A. S., Wake R. G. Restriction map of DNA spanning the replication terminus of the Bacillus subtilis chromosome. J Mol Biol. 1983 Dec 5;171(2):119–137. doi: 10.1016/s0022-2836(83)80349-0. [DOI] [PubMed] [Google Scholar]
  13. Wood W. I., Gitschier J., Lasky L. A., Lawn R. M. Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries. Proc Natl Acad Sci U S A. 1985 Mar;82(6):1585–1588. doi: 10.1073/pnas.82.6.1585. [DOI] [PMC free article] [PubMed] [Google Scholar]

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