Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1970 Aug;66(4):1252–1259. doi: 10.1073/pnas.66.4.1252

PROMITOCHONDRIA OF ANAEROBICALLY GROWN YEAST, IV. CONVERSION INTO RESPIRING MITOCHONDRIA*

H Plattner 1, M M Salpeter 1, J Saltzgaber 1, G Schatz 1,†
PMCID: PMC335813  PMID: 4319885

Abstract

Promitochondria of anaerobically grown Saccharomyces cerevisiae were selectively labeled in vivo by incubating the cells with [3H]leucine and cycloheximide. When the labeled cells were washed free of cycloheximide and adapted to oxygen in the presence of unlabeled leucine, the respiring mitochondria formed during adaptation proved to be radioactive. In contrast, only insignificant label was found in iso-1-cytochrome c which is synthesized de novo during adaptation. Respiratory adaptation of anaerobically grown yeast thus involves differentiation of promitochondrial organelles.

Full text

PDF
1257

Images in this article

1256Image
on p.1256
1257Image
on p.1257
1258Image
on p.1258

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Criddle R. S., Schatz G. Promitochondria of anaerobically grown yeast. I. Isolation and biochemical properties. Biochemistry. 1969 Jan;8(1):322–334. doi: 10.1021/bi00829a045. [DOI] [PubMed] [Google Scholar]
  2. Damsky C. H., Nelson W. M., Claude A. Mitochondria in anaerobically-grown, lipid-limited brewer's yeast. J Cell Biol. 1969 Oct;43(1):174–179. doi: 10.1083/jcb.43.1.174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fukuhara H., Sels A. Différence entre les mécanismes de la bioxynthèse induite des iso-cytochromes c. I. Dépendance vis-à-vis des acides aminés libres. J Mol Biol. 1966 Jun;17(2):319–333. doi: 10.1016/s0022-2836(66)80144-4. [DOI] [PubMed] [Google Scholar]
  4. Paltauf F., Schatz G. Promitochondria of anaerobicallly grown yeast. II. Lipid composition. Biochemistry. 1969 Jan;8(1):335–339. doi: 10.1021/bi00829a046. [DOI] [PubMed] [Google Scholar]
  5. Pease D. C. The preservation of tissue fine structure during rapid freezing. J Ultrastruct Res. 1967 Nov;21(1):98–124. doi: 10.1016/s0022-5320(67)80008-x. [DOI] [PubMed] [Google Scholar]
  6. Plattner H., Schatz G. Promitochondria of anaerobically grown yeast. 3. Morphology. Biochemistry. 1969 Jan;8(1):339–343. doi: 10.1021/bi00829a047. [DOI] [PubMed] [Google Scholar]
  7. Rouslin W., Schatz G. Interdependence between promitochondrial and cytoplasmic protein synthesis during respiratory adaptation in baker's yeast. Biochem Biophys Res Commun. 1969 Dec 4;37(6):1002–1007. doi: 10.1016/0006-291x(69)90231-9. [DOI] [PubMed] [Google Scholar]
  8. SALPETER M. M., BACHMANN L. AUTORADIOGRAPHY WITH THE ELECTRON MICROSCOPE. A PROCEDURE FOR IMPROVING RESOLUTION, SENSITIVITY, AND CONTRAST. J Cell Biol. 1964 Aug;22:469–477. doi: 10.1083/jcb.22.2.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Schatz G. Impaired binding of mitochondrial adenosine triphosphatase in the cytoplasmic "petite" mutant of Saccharomyces cerevisiae. J Biol Chem. 1968 May 10;243(9):2192–2199. [PubMed] [Google Scholar]
  10. Schatz G., Saltzgaber J. Protein synthesis by yeast promitochondria in vivo. Biochem Biophys Res Commun. 1969 Dec 4;37(6):996–1001. doi: 10.1016/0006-291x(69)90230-7. [DOI] [PubMed] [Google Scholar]
  11. Seligman A. M., Karnovsky M. J., Wasserkrug H. L., Hanker J. S. Nondroplet ultrastructural demonstration of cytochrome oxidase activity with a polymerizing osmiophilic reagent, diaminobenzidine (DAB). J Cell Biol. 1968 Jul;38(1):1–14. doi: 10.1083/jcb.38.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sherman F., Stewart J. W., Margoliash E., Parker J., Campbell W. The structural gene for yeast cytochrome C. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1498–1504. doi: 10.1073/pnas.55.6.1498. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. VENABLE J. H., COGGESHALL R. A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY. J Cell Biol. 1965 May;25:407–408. doi: 10.1083/jcb.25.2.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wintersberger E., Viehhauser G. Function of mitochondrial DNA in yeast. Nature. 1968 Nov 16;220(5168):699–702. doi: 10.1038/220699b0. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES