Abstract
Mycoplasmas require an external source of sterol for growth. For Mycoplasma capricolum this requirement is met not only by cholesterol but also by the methylcholestane derivatives lanosterol, cycloartenol, 4,4-dimethylcholesterol, and 4beta-methylcholestanol. Cholesteryl methyl ether and 3alpha-methylcholestanol serve equally well as sterol supplements. None of the growth-supporting sterol derivatives tested was metabolically modified. The unusual acceptance of diverse cholestane derivatives by a mycoplasma species contrasts with the structural attributes thought to be necessary for sterol function in eukaryotic membranes.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Archer D. B. Modification of the membrane composition of Mycoplasma mycoides subsp. capri by the growth medium. J Gen Microbiol. 1975 Jun;88(2):329–338. doi: 10.1099/00221287-88-2-329. [DOI] [PubMed] [Google Scholar]
- Bird C. W., Lynch J. M., Pirt F. J., Reid W. W. Steroids and squalene in Methylococcus capsulatus grown on methane. Nature. 1971 Apr 16;230(5294):473–474. doi: 10.1038/230473a0. [DOI] [PubMed] [Google Scholar]
- Bouvier P., Rohmer M., Benveniste P., Ourisson G. Delta8(14)-steroids in the bacterium Methylococcus capsulatus. Biochem J. 1976 Nov;159(2):267–271. doi: 10.1042/bj1590267. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brockerhoff H. Model of interaction of polar lipids, cholesterol, and proteins in biological membranes. Lipids. 1974 Sep;9(9):645–650. doi: 10.1007/BF02532169. [DOI] [PubMed] [Google Scholar]
- CLARK A. J., BLOCK K. The absence of sterol synthesis in insects. J Biol Chem. 1959 Oct;234:2578–2582. [PubMed] [Google Scholar]
- Chang T. Y., Telakowski C., Heuvel W. V., Alberts A. W., Vagelos P. R. Isolation and partial characterization of a cholesterol-requiring mutant of Chinese hamster ovary cells. Proc Natl Acad Sci U S A. 1977 Mar;74(3):832–836. doi: 10.1073/pnas.74.3.832. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Demel R. A., De Kruyff B. The function of sterols in membranes. Biochim Biophys Acta. 1976 Oct 26;457(2):109–132. doi: 10.1016/0304-4157(76)90008-3. [DOI] [PubMed] [Google Scholar]
- EDWARD D. G., FITZGERALD W. A. Cholesterol in the growth of organisms of the pleuropneumonia group. J Gen Microbiol. 1951 Aug;5(3):576–586. doi: 10.1099/00221287-5-3-576. [DOI] [PubMed] [Google Scholar]
- Förster H. J., Biemann K., Haigh W. G., Tattrie N. H., Colvin J. R. The structure of novel C35 pentacyclic terpenes from Acetobacter xylinum. Biochem J. 1973 Sep;135(1):133–143. doi: 10.1042/bj1350133. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hewlins M. J., Ehrhardt J. D., Hirth L., Ourisson G. The conversion of [14C]cycloartenol and [14C)lanosterol into phytosterols by cultures of Nicotiana tabacum. Eur J Biochem. 1969 Mar;8(2):184–188. doi: 10.1111/j.1432-1033.1969.tb00513.x. [DOI] [PubMed] [Google Scholar]
- Oldfield E., Chapman D. Effects of cholesterol and cholesterol derivatives on hydrocarbon chain mobility in lipids. Biochem Biophys Res Commun. 1971 May 7;43(3):610–616. doi: 10.1016/0006-291x(71)90658-9. [DOI] [PubMed] [Google Scholar]
- Razin S. Physiology of mycoplasmas. Adv Microb Physiol. 1973;10:1–80. doi: 10.1016/s0065-2911(08)60086-7. [DOI] [PubMed] [Google Scholar]
- Yeagle P. L., Martin R. B., Lala A. K., Lin H. K., Bloch K. Differential effects of cholesterol and lanosterol on artificial membranes. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4924–4926. doi: 10.1073/pnas.74.11.4924. [DOI] [PMC free article] [PubMed] [Google Scholar]