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. 1978 Sep;75(9):4257–4261. doi: 10.1073/pnas.75.9.4257

3'-terminal nucleotide sequence of encephalomyocarditis virus RNA determined by reverse transcriptase and chain-terminating inhibitors.

D Zimmern, P Kaesberg
PMCID: PMC336091  PMID: 81487

Abstract

We have adapted the chain-termination method for determining the nucleotide sequence of DNA of Sanger, Nicklen, and Coulson [(1977) Proc. Natl. Acad. Sci. USA 74, 5463--5467] for use with reverse transcriptase (RNA-directed DNA nucleotidyltransferase) on RNA templates. With this method and using a primer (the octanucleotide pdT7rC) directed at the 3'-terminal poly(A), we have determined a sequence of 166 residues in the genomic RNA of the picornavirus encephalomyocarditis virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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