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. 1978 Sep;75(9):4369–4373. doi: 10.1073/pnas.75.9.4369

Rat oocyte maturation in vitro: Relief of cyclic AMP inhibition by gonadotropins

Nava Dekel 1, William H Beers 1
PMCID: PMC336116  PMID: 212746

Abstract

The hormone-independent, spontaneous maturation that rat oocytes undergo in vitro can be inhibited by derivatives of cyclic AMP and inhibitors of cyclic nucleotide phosphodiesterase. In this study, we have shown that this inhibition of maturation can be partially relieved by preparations of ovine and rat luteinizing hormone or follicle-stimulating hormone. The ability of gonadotropins to foster the resumption of maturation in cultures of cyclic AMP-inhibited oocytes suggests that this system is suitable for studies of the hormonal control of oocyte development. The dose and time dependency of the response to gonadotropins has been examined in order to study the role of these hormones in oocyte maturation and to compare this effect to other known responses of the cumulus-oocyte complex. These studies show that highly purified preparations of rat gonadotropins are less effective inducers of maturation than the more commonly used, but considerably less purified, preparations of ovine gonadotropins. Almost complete relief of inhibition is observed, however, when the oocytes are exposed to a combination of rat luteinizing hormone and follicle-stimulating hormone. Oocyte maturation was not influenced by the sex steroids progesterone or 17β-estradiol. Our results suggest that: (i) cyclic AMP is involved in the intrafollicular inhibition of oocyte maturation; (ii) both gonadotropins are required for maximal stimulation of the resumption of oocyte meiosis; and (iii) steroids are not involved in this response to gonadotropins.

Keywords: dictyate, meiosis, cumulus-oocyte complex, inhibitors of cyclic nucleotide phosphodiesterase

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ayalon D., Tsafriri A., Lindner H. R., Cordova T., Harell A. Serum gonadotrophin levels in pro-oestrous rats in relation to the resumption of meiosis by the oocytes. J Reprod Fertil. 1972 Oct;31(1):51–58. doi: 10.1530/jrf.0.0310051. [DOI] [PubMed] [Google Scholar]
  2. Bauminger S., Koch Y., Khan I., Hillensjö T., Nilsson L., Ahrén K. Preovulatory changes in ovarian cyclic AMP and prostaglandins in immature rats injected with PMSG. J Reprod Fertil. 1978 Jan;52(1):21–23. doi: 10.1530/jrf.0.0520021. [DOI] [PubMed] [Google Scholar]
  3. Cho W. K., Stern S., Biggers J. D. Inhibitory effect of dibutyryl cAMP on mouse oocyte maturation in vitro. J Exp Zool. 1974 Mar;187(3):383–386. doi: 10.1002/jez.1401870307. [DOI] [PubMed] [Google Scholar]
  4. Daane T. A., Parlow A. F. Periovulatory patterns of rat serum follicle stimulating hormone and luteinizing hormone during the normal estrous cycle as revealed by radioimmunoassays: effects of pentobarbital. Endocrinology. 1971 Mar;88(3):653–663. doi: 10.1210/endo-88-3-653. [DOI] [PubMed] [Google Scholar]
  5. Dekel N., Kraicer P. F. Induction in vitro of mucification of rat cumulus oophorus by gonadotrophins and adenosine 3',5'-monophosphate. Endocrinology. 1978 Jun;102(6):1797–1802. doi: 10.1210/endo-102-6-1797. [DOI] [PubMed] [Google Scholar]
  6. Gilula N. B., Epstein M. L., Beers W. H. Cell-to-cell communication and ovulation. A study of the cumulus-oocyte complex. J Cell Biol. 1978 Jul;78(1):58–75. doi: 10.1083/jcb.78.1.58. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lawrence T. S., Beers W. H., Gilula N. B. Transmission of hormonal stimulation by cell-to-cell communication. Nature. 1978 Apr 6;272(5653):501–506. doi: 10.1038/272501a0. [DOI] [PubMed] [Google Scholar]
  8. Magnusson C., Hillensjö T. Inhibition of maturation and metabolism in rat oocytes by cyclic amp. J Exp Zool. 1977 Jul;201(1):139–147. doi: 10.1002/jez.1402010117. [DOI] [PubMed] [Google Scholar]
  9. Marsh J. M., Mills T. M., Lemaire W. J. Cyclic AMP synthesis in rabbit graafian follicles and the effect of luteinizing hormone. Biochim Biophys Acta. 1972 Jul 19;273(2):389–394. doi: 10.1016/0304-4165(72)90231-0. [DOI] [PubMed] [Google Scholar]
  10. Mason N. R., Marsh R. Cyclic AMP in the rat ovary: effect of endogenous LH secretion. Endocr Res Commun. 1975;2(4-5):357–365. doi: 10.1080/07435807509089008. [DOI] [PubMed] [Google Scholar]
  11. Nekola M. V., Smith D. M. Failure of gonadotropins to induce in vitro maturation of mouse oocytes treated with dibutyryl cyclic AMP. J Exp Zool. 1975 Dec;194(3):529–533. doi: 10.1002/jez.1401940309. [DOI] [PubMed] [Google Scholar]
  12. ODOR D. L. Electron microscopic studies on ovarian oocytes and unfertilized tubal ova in the rat. J Biophys Biochem Cytol. 1960 Jun;7:567–574. doi: 10.1083/jcb.7.3.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rondell P. Role of steroid synthesis in the process of ovulation. Biol Reprod. 1974 Mar;10(2):199–215. doi: 10.1095/biolreprod10.2.199. [DOI] [PubMed] [Google Scholar]
  14. Schuetz A. W. Role of hormones in oocyte maturation. Biol Reprod. 1974 Mar;10(2):150–178. doi: 10.1095/biolreprod10.2.150. [DOI] [PubMed] [Google Scholar]
  15. Schwartz N. B. The role of FSH and LH and of their antibodies on follicle growth and on ovulation. Biol Reprod. 1974 Mar;10(2):236–272. doi: 10.1095/biolreprod10.2.236. [DOI] [PubMed] [Google Scholar]
  16. Tsafriri A., Channing C. P. An inhibitory influence of granulosa cells and follicular fluid upon porcine oocyte meiosis in vitro. Endocrinology. 1975 Apr;96(4):922–927. doi: 10.1210/endo-96-4-922. [DOI] [PubMed] [Google Scholar]
  17. Tsafriri A., Lindner H. R., Zor U., Lamprecht S. A. In-vitro induction of meiotic division in follicle-enclosed rat oocytes by LH, cyclic AMP and prostaglandin E 2 . J Reprod Fertil. 1972 Oct;31(1):39–50. doi: 10.1530/jrf.0.0310039. [DOI] [PubMed] [Google Scholar]
  18. Tsafriri A., Pomerantz S. H., Channing C. P. Inhibition of oocyte maturation by porcine follicular fluid: partial characterization of the inhibitor. Biol Reprod. 1976 Jun;14(5):511–516. doi: 10.1095/biolreprod14.5.511. [DOI] [PubMed] [Google Scholar]

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