Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1978 Sep;75(9):4465–4469. doi: 10.1073/pnas.75.9.4465

Relative sensitivities of forward and reverse mutation assays in Salmonella typhimurium.

T R Skopek, H L Liber, D A Kaden, W G Thilly
PMCID: PMC336136  PMID: 360221

Abstract

Forward mutation to 8-azaguanine resistance and reverse mutation to histidine prototrophy were measured in Salmonella typhimurium after treatment with 16 mutagens of both base-substitution and frameshift classes. The two approaches were found to be equisensitive for all 16 mutagens--i.e., induction of significant mutation occurred at similar concentrations in the forward mutation assay and in the most sensitive of the five Ames tester strains.

Full text

PDF
4469

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ames B. N., Lee F. D., Durston W. E. An improved bacterial test system for the detection and classification of mutagens and carcinogens. Proc Natl Acad Sci U S A. 1973 Mar;70(3):782–786. doi: 10.1073/pnas.70.3.782. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ames B. N., Mccann J., Yamasaki E. Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test. Mutat Res. 1975 Dec;31(6):347–364. doi: 10.1016/0165-1161(75)90046-1. [DOI] [PubMed] [Google Scholar]
  3. Ellenberger J., Mohn G. Mutagenic activity of cyclophosphamide, ifosfamide, and trofosfamide in different genes of escherichia coli and salmonella typhimurium after biotransformation through extracts of rodent liver. Arch Toxicol. 1975;33(3):225–240. doi: 10.1007/BF00311275. [DOI] [PubMed] [Google Scholar]
  4. Macgregor J. T., Sacks L. E. The sporulation system of Bacillus subtilis as the basis of a multi-gene mutagen screening test. Mutat Res. 1976 Aug;38(4):271–286. doi: 10.1016/0165-1161(76)90151-5. [DOI] [PubMed] [Google Scholar]
  5. McCann J., Choi E., Yamasaki E., Ames B. N. Detection of carcinogens as mutagens in the Salmonella/microsome test: assay of 300 chemicals. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5135–5139. doi: 10.1073/pnas.72.12.5135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. McCann J., Spingarn N. E., Kobori J., Ames B. N. Detection of carcinogens as mutagens: bacterial tester strains with R factor plasmids. Proc Natl Acad Sci U S A. 1975 Mar;72(3):979–983. doi: 10.1073/pnas.72.3.979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Scherr G. H., Fishman M., Weaver R. H. The Mutagenicity of Some Carcinogenic Compounds for Escherichia Coli. Genetics. 1954 Mar;39(2):141–149. doi: 10.1093/genetics/39.2.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Skopek T. R., Liber H. L., Krolewski J. J., Thilly W. G. Quantitative forward mutation assay in Salmonella typhimurium using 8-azaguanine resistance as a genetic marker. Proc Natl Acad Sci U S A. 1978 Jan;75(1):410–414. doi: 10.1073/pnas.75.1.410. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES