Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1978 Sep;75(9):4489–4493. doi: 10.1073/pnas.75.9.4489

Characterization of a feline sarcoma virus-coded antigen (FOCMA-S) by radioimmunoassay.

C J Sherr, G J Todaro, A Sliski, M Essex
PMCID: PMC336141  PMID: 212755

Abstract

A radioimmunoassay has been developed that detects a unique antigen encoded by the genome of the feline sarcoma virus (FeSV). Pseudotype viral particles containing an FeSV-specific polyprotein (p85) were used both as a source of antigen and to prepare specific antisera in rabbits. Because p85 contains antigens related to two structural proteins (p15 and p12) of feline leukemia virus (FeLV), antibodies directed to these were adsorbed with purified FeLV proteins. The adsorbed rabbit antiserum bound to antigenic determinants (designated FOCMA-S) which are also present in p85 and reacted specifically in immunofluorescence tests with rat cells transformed by FeSV and with FOCMA-positive cat lymphoid tumor cells. Competition assays detect FOCMA-S in pseudotype type C viruses rescued from FeSV-transformed mink and rat cells but not in heterologous type C helper viruses or in FeLV. A crossreactive antigen was also detected in pseudotypes of Kirsten sarcoma virus. The assay permits the quantitative measurement of an FeSV-coded protein whose expression is associated with viral transformation.

Full text

PDF
4490

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benveniste R. E., Sherr C. J., Todaro G. J. Evolution of type C viral genes: origin of feline leukemia virus. Science. 1975 Nov 28;190(4217):886–888. doi: 10.1126/science.52892. [DOI] [PubMed] [Google Scholar]
  2. Bister K., Hayman M. J., Vogt P. K. Defectiveness of avian myelocytomatosis virus MC29: isolation of long-term nonproducer cultures and analysis of virus-specific polypeptide synthesis. Virology. 1977 Oct 15;82(2):431–448. doi: 10.1016/0042-6822(77)90017-4. [DOI] [PubMed] [Google Scholar]
  3. Brugge J. S., Erikson R. L. Identification of a transformation-specific antigen induced by an avian sarcoma virus. Nature. 1977 Sep 22;269(5626):346–348. doi: 10.1038/269346a0. [DOI] [PubMed] [Google Scholar]
  4. Essex M. Immunity to leukemia, lymphoma, and fibrosarcoma in cats: a case for immunosurveillance. Contemp Top Immunobiol. 1977;6:71–106. doi: 10.1007/978-1-4684-3051-6_2. [DOI] [PubMed] [Google Scholar]
  5. Essex M., Jakowski R. M., Hardy W. D., Jr, Cotter S. M., Hess P., Sliski A. Feline oncornavirus-associated cell membrane antigen. III. Antibody titers in cats from leukemia cluster households. J Natl Cancer Inst. 1975 Mar;54(3):637–641. [PubMed] [Google Scholar]
  6. Essex M., Klein G., Snyder S. P., Harrold J. B. Correlation between humoral antibody and regression of tumours induced by feline sarcoma virus. Nature. 1971 Sep 17;233(5316):195–196. doi: 10.1038/233195a0. [DOI] [PubMed] [Google Scholar]
  7. Essex M., Sliski A., Hardy W. D., Jr, Cotter S. M. Immune response to leukemia virus and tumor-associated antigens in cats. Cancer Res. 1976 Feb;36(2 Pt 2):640–645. [PubMed] [Google Scholar]
  8. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Handa H., Shiroki K., Shimojo H. Helper factor(s) for growth of adeno-associated virus in cells transformed by adenovirus 12. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4508–4510. doi: 10.1073/pnas.74.10.4508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hardy W. D., Jr, Zuckerman E. E., MacEwen E. G., Hayes A. A., Essex M. A feline leukaemia virus- and sarcoma virus-induced tumour-specific antigen. Nature. 1977 Nov 17;270(5634):249–251. doi: 10.1038/270249a0. [DOI] [PubMed] [Google Scholar]
  11. Henderson I. C., Lieber M. M., Todaro G. J. Mink cell line Mv 1 Lu (CCL 64). Focus formation and the generation of "nonproducer" transformed cell lines with murine and feline sarcoma viruses. Virology. 1974 Jul;60(1):282–287. doi: 10.1016/0042-6822(74)90386-9. [DOI] [PubMed] [Google Scholar]
  12. Khan A. S., Stephenson J. R. Feline leukemia virus: biochemical and immunological characterization of gag gene-coded structural proteins. J Virol. 1977 Sep;23(3):599–607. doi: 10.1128/jvi.23.3.599-607.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kopchick J. J., Jamjoom G. A., Watson K. F., Arlinghaus R. B. Biosynthesis of reverse transcriptase from Rauscher murine leukemia virus by synthesis and cleavage of a gag-pol read-through viral precursor polyprotein. Proc Natl Acad Sci U S A. 1978 Apr;75(4):2016–2020. doi: 10.1073/pnas.75.4.2016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Okasinski G. F., Velicer L. F. Analysis of intracellular feline leukemia virus proteins II. Generation of feline leukemia virus structural proteins from precursor polypeptides. J Virol. 1977 Apr;22(1):74–85. doi: 10.1128/jvi.22.1.74-85.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Purchio A. F., Erikson E., Brugge J. S., Erikson R. L. Identification of a polypeptide encoded by the avian sarcoma virus src gene. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1567–1571. doi: 10.1073/pnas.75.3.1567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Scolnick E. M., Goldberg R. J., Williams D. Characterizatiion of rat genetic sequences of Kirsten sarcoma virus: distinct class of endogenous rat type C viral sequences. J Virol. 1976 May;18(2):559–566. doi: 10.1128/jvi.18.2.559-566.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Scolnick E. M., Parks W. P., Livingston D. M. Radioimmunoassay of mammalian type C viral proteins. I. Species specific reactions of murine and feline viruses. J Immunol. 1972 Sep;109(3):570–577. [PubMed] [Google Scholar]
  19. Sen A., Sherr C. J., Todaro G. J. Specific binding of the type C viral core protein p12 with purified viral RNA. Cell. 1976 Jan;7(1):21–32. doi: 10.1016/0092-8674(76)90251-8. [DOI] [PubMed] [Google Scholar]
  20. Shapiro A. L., Scharff M. D., Maizel J. V., Jr, Uhr J. W. Polyribosomal synthesis and assembly of the H and L chains of gamma globulin. Proc Natl Acad Sci U S A. 1966 Jul;56(1):216–221. doi: 10.1073/pnas.56.1.216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sherr C. J., Benveniste R. E., Todaro G. J. Endogenous mink (Mustela vison) type C virus isolated from sarcoma virus-transformed mink cells. J Virol. 1978 Mar;25(3):738–749. doi: 10.1128/jvi.25.3.738-749.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sherr C. J., Sen A., Todaro G. J., Sliski A., Essex M. Pseudotypes of feline sarcoma virus contain an 85,000-dalton protein with feline oncornavirus-associated cell membrane antigen (FOCMA) activity. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1505–1509. doi: 10.1073/pnas.75.3.1505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sherr C. J., Todaro G. J. Radioimmunoassay of the major group specific protein of endogenous baboon type C viruses: relation to the RD-114-CCC group and detection of antigen in normal baboon tissues. Virology. 1974 Sep;61(1):168–181. doi: 10.1016/0042-6822(74)90252-9. [DOI] [PubMed] [Google Scholar]
  24. Sliski A. H., Essex M., Meyer C., Todaro G. Feline oncornavirus-associated cell membrane antigen: expression in transformed nonproducer mink cells. Science. 1977 Jun 17;196(4296):1336–1339. doi: 10.1126/science.194310. [DOI] [PubMed] [Google Scholar]
  25. Strand M., August J. T. Structural proteins of oncogenic ribonucleic acid viruses. Interspec II, a new interspecies antigen. J Biol Chem. 1973 Aug 25;248(16):5627–5633. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES