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. 1978 Oct;75(10):4661–4663. doi: 10.1073/pnas.75.10.4661

A photochemical technique for measuring lateral diffusion of spin-labeled phospholipids in membranes

James R Sheats 1, Harden M McConnell 1,*
PMCID: PMC336176  PMID: 16592571

Abstract

A technique is described for the measurement of lateral diffusion of any spin-labeled molecule in planar phospholipid multilayers, using low probe concentrations and a photochemical reaction to generate the initial concentration gradient. Features of the technique include simplicity of data analysis, versatility, and applicability over a wide range of motional rates. Measurements have been made on dimyristoyl phosphatidylcholine and dipalmitoyl phosphatidylcholine in the fluid phase (using a phospholipid with head-group spin label), and excellent agreement is found with recent nuclear magnetic resonance spin-echo measurements, as well as with other spin-label and fluorescence photobleaching results.

Keywords: nitroxides, photobleaching, lipid bilayers, translational motion, alkylcobaltate

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berg H. C., Purcell E. M. Physics of chemoreception. Biophys J. 1977 Nov;20(2):193–219. doi: 10.1016/S0006-3495(77)85544-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brûlet P., McConnell H. M. Lateral hapten mobility and immunochemistry of model membranes. Proc Natl Acad Sci U S A. 1976 Sep;73(9):2977–2981. doi: 10.1073/pnas.73.9.2977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brûlet P., McConnell H. M. Magnetic resonance spectra of membranes. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1451–1455. doi: 10.1073/pnas.72.4.1451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brûlet P., McConnell H. M. Structural and dynamical aspects of membrane immunochemistry using model membranes. Biochemistry. 1977 Mar 22;16(6):1209–1217. doi: 10.1021/bi00625a028. [DOI] [PubMed] [Google Scholar]
  5. Devaux P., McConnell H. M. Lateral diffusion in spin-labeled phosphatidylcholine multilayers. J Am Chem Soc. 1972 Jun 28;94(13):4475–4481. doi: 10.1021/ja00768a600. [DOI] [PubMed] [Google Scholar]
  6. Edelman G. M. Surface modulation in cell recognition and cell growth. Science. 1976 Apr 16;192(4236):218–226. doi: 10.1126/science.769162. [DOI] [PubMed] [Google Scholar]
  7. Nicolson G. L. Transmembrane control of the receptors on normal and tumor cells. I. Cytoplasmic influence over surface components. Biochim Biophys Acta. 1976 Apr 13;457(1):57–108. doi: 10.1016/0304-4157(76)90014-9. [DOI] [PubMed] [Google Scholar]
  8. Rey P., McConnell H. M. Clustering of nitroxide spin labels in lipid bilayer membranes. J Am Chem Soc. 1977 Mar 2;99(5):1637–1642. doi: 10.1021/ja00447a059. [DOI] [PubMed] [Google Scholar]
  9. Sackmann E., Träuble H. Studies of the crystalline-liquid crystalline phase transition of lipid model membranes. II. Analysis of electron spin resonance spectra of steroid labels incorporated into lipid membranes. J Am Chem Soc. 1972 Jun 28;94(13):4492–4498. doi: 10.1021/ja00768a014. [DOI] [PubMed] [Google Scholar]
  10. Saffman P. G., Delbrück M. Brownian motion in biological membranes. Proc Natl Acad Sci U S A. 1975 Aug;72(8):3111–3113. doi: 10.1073/pnas.72.8.3111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schlessinger J., Webb W. W., Elson E. L., Metzger H. Lateral motion and valence of Fc receptors on rat peritoneal mast cells. Nature. 1976 Dec 9;264(5586):550–552. doi: 10.1038/264550a0. [DOI] [PubMed] [Google Scholar]
  12. Segal D. M., Taurog J. D., Metzger H. Dimeric immunoglobulin E serves as a unit signal for mast cell degranulation. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2993–2997. doi: 10.1073/pnas.74.7.2993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shimshick E. J., McConnell H. M. Lateral phase separation in phospholipid membranes. Biochemistry. 1973 Jun 5;12(12):2351–2360. doi: 10.1021/bi00736a026. [DOI] [PubMed] [Google Scholar]
  14. Smith B. A., McConnell H. M. Determination of molecular motion in membranes using periodic pattern photobleaching. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2759–2763. doi: 10.1073/pnas.75.6.2759. [DOI] [PMC free article] [PubMed] [Google Scholar]

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