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. 1978 Oct;75(10):4661–4663. doi: 10.1073/pnas.75.10.4661

A photochemical technique for measuring lateral diffusion of spin-labeled phospholipids in membranes

James R Sheats 1, Harden M McConnell 1,*
PMCID: PMC336176  PMID: 16592571

Abstract

A technique is described for the measurement of lateral diffusion of any spin-labeled molecule in planar phospholipid multilayers, using low probe concentrations and a photochemical reaction to generate the initial concentration gradient. Features of the technique include simplicity of data analysis, versatility, and applicability over a wide range of motional rates. Measurements have been made on dimyristoyl phosphatidylcholine and dipalmitoyl phosphatidylcholine in the fluid phase (using a phospholipid with head-group spin label), and excellent agreement is found with recent nuclear magnetic resonance spin-echo measurements, as well as with other spin-label and fluorescence photobleaching results.

Keywords: nitroxides, photobleaching, lipid bilayers, translational motion, alkylcobaltate

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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