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. 1978 Oct;75(10):4759–4763. doi: 10.1073/pnas.75.10.4759

Circular dichroism analysis of mononucleosome DNA conformation.

M K Cowman, G D Fasman
PMCID: PMC336199  PMID: 283389

Abstract

Mononucleosomes were isolated from micrococcal nuclease digests of chicken erythrocyte nuclei. The circular dichroism properties of mononucleosome preparations, differing in average DNA length and in H1 and H5 content, demonstrate that the spectrum of chromatin is due only to the complete structure of its repeating subunits. The nucleoprotein spectra are all altered relative to protein-free DNA by the emergence of a single negative band at 275 nm, similar to the band observed for psi DNA. The intensity of the psi-type band depends on the proportion of DNA condensed in a specific manner. The psi-type band is proposed to be due to the compact DNA tertiary structure; i.e., the manner in which the DNA is wound around the histone core allowing interactions between adjacent turns of the superhelix. This interpretation attributes changes and variability in nucleoprotein circular dichroism spectra under different experimental conditions to alterations in DNA tertiary structure rather than secondary structure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartley J., Chalkley R. An approach to the structure of native nucleohistone. Biochemistry. 1973 Jan 30;12(3):468–474. doi: 10.1021/bi00727a017. [DOI] [PubMed] [Google Scholar]
  2. Bram S. The secondary structure of DNA in solution and in nucleohistone. J Mol Biol. 1971 May 28;58(1):277–288. doi: 10.1016/0022-2836(71)90246-4. [DOI] [PubMed] [Google Scholar]
  3. Brasch K. Studies on the role of histones HI (f1) and H5 (f2c) in chromatin structure. Exp Cell Res. 1976 Sep;101(2):396–410. doi: 10.1016/0014-4827(76)90391-8. [DOI] [PubMed] [Google Scholar]
  4. Britten R. J., Graham D. E., Neufeld B. R. Analysis of repeating DNA sequences by reassociation. Methods Enzymol. 1974;29:363–418. doi: 10.1016/0076-6879(74)29033-5. [DOI] [PubMed] [Google Scholar]
  5. Fasman G. D., Schaffhausen B., Goldsmith L., Adler A. Conformational changes associated with f-1 histone-deoxyribonucleic acid complexes. Circular dichroism studies. Biochemistry. 1970 Jul 7;9(14):2814–2822. doi: 10.1021/bi00816a010. [DOI] [PubMed] [Google Scholar]
  6. Felsenfeld G. Chromatin. Nature. 1978 Jan 12;271(5641):115–122. doi: 10.1038/271115a0. [DOI] [PubMed] [Google Scholar]
  7. Finch J. T., Klug A. Solenoidal model for superstructure in chromatin. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1897–1901. doi: 10.1073/pnas.73.6.1897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Finch J. T., Lutter L. C., Rhodes D., Brown R. S., Rushton B., Levitt M., Klug A. Structure of nucleosome core particles of chromatin. Nature. 1977 Sep 1;269(5623):29–36. doi: 10.1038/269029a0. [DOI] [PubMed] [Google Scholar]
  9. Goodwin D. C., Brahms J. Form of DNA and the nature of interactions with proteins in chromatin. Nucleic Acids Res. 1978 Mar;5(3):835–850. doi: 10.1093/nar/5.3.835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gray D. M., Taylor T. N., Lang D. Dehydrated circular DNA: circular dichroism of molecules in ethanolic solutions. Biopolymers. 1978 Jan;17(1):145–157. doi: 10.1002/bip.1978.360170111. [DOI] [PubMed] [Google Scholar]
  11. Haynes M., Garrett R. A., Gratzer W. B. Structure of nucleic acid-poly base complexes. Biochemistry. 1970 Oct 27;9(22):4410–4416. doi: 10.1021/bi00824a600. [DOI] [PubMed] [Google Scholar]
  12. Jordan C. F., Lerman L. S., Venable J. H. Structure and circular dichroism of DNA in concentrated polymer solutions. Nat New Biol. 1972 Mar 22;236(64):67–70. doi: 10.1038/newbio236067a0. [DOI] [PubMed] [Google Scholar]
  13. Klevan L., Crothers D. M. Isolation and characterization of a spacerless dinucleosome from H1-deleted chromatin. Nucleic Acids Res. 1977 Dec;4(12):4077–4089. doi: 10.1093/nar/4.12.4077. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kornberg R. D. Structure of chromatin. Annu Rev Biochem. 1977;46:931–954. doi: 10.1146/annurev.bi.46.070177.004435. [DOI] [PubMed] [Google Scholar]
  15. Lewis E. A., DeBuysere M. S., Rees A. W. Configuration of unsheared nucleohistone. Effects of ionic strength and of histone F1 removal. Biochemistry. 1976 Jan 13;15(1):186–192. doi: 10.1021/bi00646a029. [DOI] [PubMed] [Google Scholar]
  16. Loening U. E. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967 Jan;102(1):251–257. doi: 10.1042/bj1020251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mandel R., Fasman G. D. Chromatin and nucleosome structure. Nucleic Acids Res. 1976 Aug;3(8):1839–1855. doi: 10.1093/nar/3.8.1839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mandel R., Fasman G. D. Thermal denaturation of DNA and DNA:polypeptide complexes. Simultaneous absorption and circular dichroism measurements. Biochem Biophys Res Commun. 1974 Jul 24;59(2):672–679. doi: 10.1016/s0006-291x(74)80032-x. [DOI] [PubMed] [Google Scholar]
  19. Maniatis T., Jeffrey A., van deSande H. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975 Aug 26;14(17):3787–3794. doi: 10.1021/bi00688a010. [DOI] [PubMed] [Google Scholar]
  20. Maniatis T., Venable J. H., Jr, Lerman L. S. The structure of psi DNA. J Mol Biol. 1974 Mar 25;84(1):37–64. doi: 10.1016/0022-2836(74)90211-3. [DOI] [PubMed] [Google Scholar]
  21. Nicolini C., Baserga R., Kendall F. DNA structure in sheared and unsheared chromatin. Science. 1976 May 21;192(4241):796–798. doi: 10.1126/science.1265482. [DOI] [PubMed] [Google Scholar]
  22. Olins A. L., Olins D. E. Spheroid chromatin units (v bodies). Science. 1974 Jan 25;183(4122):330–332. doi: 10.1126/science.183.4122.330. [DOI] [PubMed] [Google Scholar]
  23. Peacock A. C., Dingman C. W. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis. Biochemistry. 1967 Jun;6(6):1818–1827. doi: 10.1021/bi00858a033. [DOI] [PubMed] [Google Scholar]
  24. Renz M., Nehls P., Hozier J. Involvement of histone H1 in the organization of the chromosome fiber. Proc Natl Acad Sci U S A. 1977 May;74(5):1879–1883. doi: 10.1073/pnas.74.5.1879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Shaw B. R., Herman T. M., Kovacic R. T., Beaudreau G. S., Van Holde K. E. Analysis of subunit organization in chicken erythrocyte chromatin. Proc Natl Acad Sci U S A. 1976 Feb;73(2):505–509. doi: 10.1073/pnas.73.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Shih T. Y., Fasman G. D. Conformation of deoxyribonucleic acid in chromatin: a circular dichroism study. J Mol Biol. 1970 Aug 28;52(1):125–129. doi: 10.1016/0022-2836(70)90182-8. [DOI] [PubMed] [Google Scholar]
  27. Shih T. Y., Lake R. S. Studies on the structure of metaphase and interphase chromatin of Chinese hamster cells by circular dichroism and thermal denaturation. Biochemistry. 1972 Dec 5;11(25):4811–4817. doi: 10.1021/bi00775a026. [DOI] [PubMed] [Google Scholar]
  28. Slayter H. S., Shih T. Y., Adler A. J., Fasman G. D. Electron microscopy and circular dichroism studies on chromatin. Biochemistry. 1972 Aug 1;11(16):3044–3054. doi: 10.1021/bi00766a016. [DOI] [PubMed] [Google Scholar]
  29. Tatchell K., Van Holde K. E. Compact oligomers and nucleosome phasing. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3583–3587. doi: 10.1073/pnas.75.8.3583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Tatchell K., Van Holde K. E. Reconstitution of chromatin core particles. Biochemistry. 1977 Nov 29;16(24):5295–5303. doi: 10.1021/bi00643a021. [DOI] [PubMed] [Google Scholar]
  31. Thoma F., Koller T. Influence of histone H1 on chromatin structure. Cell. 1977 Sep;12(1):101–107. doi: 10.1016/0092-8674(77)90188-x. [DOI] [PubMed] [Google Scholar]
  32. Tunis-Schneider M. J., Maestre M. F. Circular dichroism spectra of oriented and unoriented deoxyribonucleic acid films--a preliminary study. J Mol Biol. 1970 Sep 28;52(3):521–541. doi: 10.1016/0022-2836(70)90417-1. [DOI] [PubMed] [Google Scholar]
  33. Whitlock J. P., Jr, Simpson R. T. Preparation and physical characterization of a homogeneous population of monomeric nucleosomes from HeLa cells. Nucleic Acids Res. 1976 Sep;3(9):2255–2266. doi: 10.1093/nar/3.9.2255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. de Murcia G., Das G. C., Erard M., Daune M. Superstructure and CD spectrum as probes of chromatin integrity. Nucleic Acids Res. 1978 Feb;5(2):523–535. doi: 10.1093/nar/5.2.523. [DOI] [PMC free article] [PubMed] [Google Scholar]

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