Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1978 Oct;75(10):5137–5141. doi: 10.1073/pnas.75.10.5137

Spontaneous and antibody-dependent cell-mediated cytotoxicity by human T cell subpopulations.

S Gupta, G Fernandes, M Nair, R A Good
PMCID: PMC336279  PMID: 311006

Abstract

Human peripheral blood non-T cells, T cells and their subpopulations (Tmu, Tgamma, Tphi, Tgamma-depleted cells, and Tmu-depleted cells) were assayed for their capacity to mediated spontaneous lymphocyte-mediated cytotoxicity (SLMC) or natural killer activity against K562 tumor cell line and antibody-dependent cellular cytotoxicity (ADCC) against chicken erythrocytes coated with antibody. Non-T cells, unseparated T cells, Tgamma cells, and Tmu-depleted (Tgamma-enriched) cells were found to have both SLMC (NK activity) and ADCC. Tmu, Tphi, and Tgamma-depleted cells had minimal or no SLMC and ADCC activity. This study demonstrates that SLMC and ADCC activity in T cells is mediated by Tgamma cell subpopulations. These two cytotoxic reactions were either mediated by two distinct subsets of Tgamma cells or by a single effector cell using two different mechanisms.

Full text

PDF
5141

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akira D., Takasugi M. Loss of specific natural cell-mediated cytotoxicity with absorption of natural antibodies from serum. Int J Cancer. 1977 Jun 15;19(6):747–755. doi: 10.1002/ijc.2910190603. [DOI] [PubMed] [Google Scholar]
  2. Behelak Y., Banerjee D., Richter M. Immunocompetent cells in patients with malignant disease. I. The lack of naturally occurring killer cell activity in the unfractionated circulating lymphocytes from patients with chronic lymphocytic leukemia (CLL). Cancer. 1976 Dec;38(6):2274–2277. doi: 10.1002/1097-0142(197612)38:6<2274::aid-cncr2820380614>3.0.co;2-i. [DOI] [PubMed] [Google Scholar]
  3. Cordier G., Samarut C., Brochier J., Revillard J. P. Antibody-dependent cell cytoxicity (ADCC). Characterization of 'killer' cells in human lymphoid organs. Scand J Immunol. 1976;5(3):233–242. doi: 10.1111/j.1365-3083.1976.tb00274.x. [DOI] [PubMed] [Google Scholar]
  4. Cordier G., Samarut C., Revillard J. P. Changes of Fcgamma receptor-related properties induced by interaction of human lymphocytes with insoluble immune complexes. J Immunol. 1977 Dec;119(6):1943–1948. [PubMed] [Google Scholar]
  5. Eremin O., Coombs R. R., Plumb D., Ashby J. Characterization of the human natural killer (NK) cell in blood and lymphoid organs. Int J Cancer. 1978 Jan 15;21(1):42–50. doi: 10.1002/ijc.2910210109. [DOI] [PubMed] [Google Scholar]
  6. Fernandes G., Yunis E. J., Good R. A. Depression of cytotoxic T cell subpopulation in mice by hydrocortisone treatment. Clin Immunol Immunopathol. 1975 Jul;4(2):304–313. doi: 10.1016/0090-1229(75)90066-5. [DOI] [PubMed] [Google Scholar]
  7. Grossi C. E., Webb S. R., Zicca A., Lydyard P. M., Moretta L., Mingari M. C., Cooper M. D. Morphological and histochemical analyses of two human T-cell subpopulations bearing receptors for IgM or IgG. J Exp Med. 1978 May 1;147(5):1405–1417. doi: 10.1084/jem.147.5.1405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gupta S., Good R. A. Subpopulations of human T lymphocytes. I. Studies in immunodeficient patients. Clin Exp Immunol. 1977 Nov;30(2):222–228. [PMC free article] [PubMed] [Google Scholar]
  9. Gupta S., Good R. A. Subpopulations of human T lymphocytes. II. Effect of thymopoietin, corticosteroids, and irradiation. Cell Immunol. 1977 Nov;34(1):10–18. doi: 10.1016/0008-8749(77)90224-6. [DOI] [PubMed] [Google Scholar]
  10. Gupta S., Good R. A. Subpopulations of human T lymphocytes. III. Distribution and quantitation in peripheral blood, cord blood, tonsils, bone marrow, thymus, lymph nodes, and spleen. Cell Immunol. 1978 Mar 15;36(2):263–270. doi: 10.1016/0008-8749(78)90270-8. [DOI] [PubMed] [Google Scholar]
  11. Gupta S., Safai B., Good R. A. Subpopulations of human T lymphocytes. IV. Quantitation and distribution in patients with mycosis fungoides and Sézary syndrome. Cell Immunol. 1978 Aug;39(1):18–26. doi: 10.1016/0008-8749(78)90078-3. [DOI] [PubMed] [Google Scholar]
  12. Handwerger B. S., Koren H. S. The nature of the effector cell in antibody-dependent, cell-mediated cytolysis (ADCC): the cytotoxic activity of murine tumor cells and peritoneal macrophages. Clin Immunol Immunopathol. 1976 Mar;5(2):272–281. doi: 10.1016/0090-1229(76)90032-5. [DOI] [PubMed] [Google Scholar]
  13. Hellström I., Hellström K. E., Sjögren H. O., Warner G. A. Demonstration of cell-mediated immunity to human neoplasms of various histological types. Int J Cancer. 1971 Jan 15;7(1):1–16. doi: 10.1002/ijc.2910070102. [DOI] [PubMed] [Google Scholar]
  14. Jondal M., Pross H. Surface markers on human b and t lymphocytes. VI. Cytotoxicity against cell lines as a functional marker for lymphocyte subpopulations. Int J Cancer. 1975 Apr 15;15(4):596–605. doi: 10.1002/ijc.2910150409. [DOI] [PubMed] [Google Scholar]
  15. Kay H. D., Bonnard G. D., West W. H., Herberman R. B. A functional comparison of human Fc-receptor-bearing lymphocytes active in natural cytotoxicity and antibody-dependent cellular cytotoxicity. J Immunol. 1977 Jun;118(6):2058–2066. [PubMed] [Google Scholar]
  16. Klein E., Ben-Bassat H., Neumann H., Ralph P., Zeuthen J., Polliack A., Vánky F. Properties of the K562 cell line, derived from a patient with chronic myeloid leukemia. Int J Cancer. 1976 Oct 15;18(4):421–431. doi: 10.1002/ijc.2910180405. [DOI] [PubMed] [Google Scholar]
  17. Koide Y., Takasugi M., Biling R. B cell antigens on effectors for natural cell-mediated cytotoxicity. Eur J Immunol. 1977 Oct;7(10):731–736. doi: 10.1002/eji.1830071016. [DOI] [PubMed] [Google Scholar]
  18. Koren H. S., Amos D. B., Buckley R. H. Natural killing in immunodeficient patients. J Immunol. 1978 Mar;120(3):796–799. [PubMed] [Google Scholar]
  19. Moretta L., Ferrarini M., Mingari M. C., Moretta A., Webb S. R. Subpopulations of human T cells identified by receptors for immunoglobulins and mitogen responsiveness. J Immunol. 1976 Dec;117(6):2171–2174. [PubMed] [Google Scholar]
  20. Moretta L., Mingari M. C., Moretta A., Lydyard P. M. Receptors for IgM are expressed on acute lymphoblastic leukemic cells having T-cell characteristics. Clin Immunol Immunopathol. 1977 May;7(3):405–409. doi: 10.1016/0090-1229(77)90075-7. [DOI] [PubMed] [Google Scholar]
  21. Moretta L., Mingari M. C., Romanzi C. A. Loss of Fc receptors for IgG from human T lymphocytes exposed to IgG immune complexes. Nature. 1978 Apr 13;272(5654):618–620. doi: 10.1038/272618a0. [DOI] [PubMed] [Google Scholar]
  22. Moretta L., Mingari M. C., Webb S. R., Pearl E. R., Lydyard P. M., Grossi C. E., Lawton A. R., Cooper M. D. Imbalances in T cell subpopulations associated with immunodeficiency and autoimmune syndromes. Eur J Immunol. 1977 Oct;7(10):696–700. doi: 10.1002/eji.1830071009. [DOI] [PubMed] [Google Scholar]
  23. Moretta L., Webb S. R., Grossi C. E., Lydyard P. M., Cooper M. D. Functional analysis of two human T-cell subpopulations: help and suppression of B-cell responses by T cells bearing receptors for IgM or IgG. J Exp Med. 1977 Jul 1;146(1):184–200. doi: 10.1084/jem.146.1.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Nelson D. L., Bundy B. M., Pitchon H. E., Blaese R. M., Strober W. The effector cells in human peripheral blood mediating mitogen-induced cellular cytotoxicity and antibody-dependent cellular cytotoxicity. J Immunol. 1976 Nov;117(5 Pt 1):1472–1481. [PubMed] [Google Scholar]
  25. Nelson D. L., Bundy B. M., Strober W. Spontaneous cell-mediated cytotoxicity by human peripheral blood lymphocytes in vitro. J Immunol. 1977 Oct;119(4):1401–1405. [PubMed] [Google Scholar]
  26. Pape G. R., Troye M., Perlmann P. Characterization of cytolytic effector cells in peripheral blood of healthy individuals and cancer patients. I. Surface markers and K cell activity after separation of B cells and lymphocytes and Fc-receptors by column fractionation. J Immunol. 1977 Jun;118(6):1919–1924. [PubMed] [Google Scholar]
  27. Parrott D. M., Good R. A., O'Neill G. J., Gupta S. Heterogeneity of locomotion in human T cell subsets. Proc Natl Acad Sci U S A. 1978 May;75(5):2392–2395. doi: 10.1073/pnas.75.5.2392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Perlmann P., Holm G. Cytotoxic effects of lymphoid cells in vitro. Adv Immunol. 1969;11:117–193. doi: 10.1016/s0065-2776(08)60479-4. [DOI] [PubMed] [Google Scholar]
  29. Perlmann P., Perlmann H. Contactual lysis of antibody-coated chicken erythrocytes by purified lymphocytes. Cell Immunol. 1970 Sep;1(3):300–315. doi: 10.1016/0008-8749(70)90051-1. [DOI] [PubMed] [Google Scholar]
  30. Pross H. F., Baines M. G., Jondal M. Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells. II. Is the complement receptor necessarily present on the killer cells? Int J Cancer. 1977 Sep 15;20(3):353–358. doi: 10.1002/ijc.2910200306. [DOI] [PubMed] [Google Scholar]
  31. Pross H. F., Baines M. G. Spontaneous human lymphocyte-mediated cytotoxicity againts tumour target cells. I. The effect of malignant disease. Int J Cancer. 1976 Nov 15;18(5):593–604. doi: 10.1002/ijc.2910180508. [DOI] [PubMed] [Google Scholar]
  32. Pross H. F., Jondal M. Cytotoxic lymphocytes from normal donors. A functional marker of human non-T lymphocytes. Clin Exp Immunol. 1975 Aug;21(2):226–235. [PMC free article] [PubMed] [Google Scholar]
  33. Rosenberg E. B., McCoy J. L., Green S. S., Donnelly F. C., Siwarski D. F., Levine P. H., Herberman R. B. Destruction of human lymphoid tissue-culture cell lines by human peripheral lymphocytes in 51Cr-release cellular cytotoxicity assays. J Natl Cancer Inst. 1974 Feb;52(2):345–352. doi: 10.1093/jnci/52.2.345. [DOI] [PubMed] [Google Scholar]
  34. Sanal S. O., Buckley R. H. Antibody-dependent cellular cytotoxicity in primary immunodeficiency diseases and with normal leukocyte subpopulations. Importance of the type of target. J Clin Invest. 1978 Jan;61(1):1–10. doi: 10.1172/JCI108907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Takasugi M., Ward P. H., Mickey M. R., Terasaki P. I. Allogeneic cell-mediated testing for human tumor antigens. Natl Cancer Inst Monogr. 1972 Dec;35:251–257. [PubMed] [Google Scholar]
  36. Ting A., Terasaki P. I. Depressed lymphocyte-mediated killing of sensitized targets in cancer patients. Cancer Res. 1974 Oct;34(10):2694–2698. [PubMed] [Google Scholar]
  37. Troye M., Perlmann P., Pape G. R., Spiegelberg H. L., Näslund I., Gidlöf A. The use of Fab fragments of anti-human immunoglobulin as analytic tools for establishing the involvement of immunoglobulin in the spontaneous cytotoxicity to cultured tumor cells by lymphocytes from patients with bladder carcinoma and from healthy donors. J Immunol. 1977 Sep;119(3):1061–1067. [PubMed] [Google Scholar]
  38. West W. H., Boozer R. B., Herberman R. B. Low affinity E-rosette formation by the human K cell. J Immunol. 1978 Jan;120(1):90–95. [PubMed] [Google Scholar]
  39. West W. H., Cannon G. B., Kay H. D., Bonnard G. D., Herberman R. B. Natural cytotoxic reactivity of human lymphocytes against a myeloid cell line: characterization of effector cells. J Immunol. 1977 Jan;118(1):355–361. [PubMed] [Google Scholar]
  40. Wisloff F., Froland S. S., Michaelsen T. E. Antibody-dependent cytotoxicity mediated by human Fc-receptor-bearing cells lacking markers for B- and T-lymphocytes. Int Arch Allergy Appl Immunol. 1974;47(1):139–154. doi: 10.1159/000231208. [DOI] [PubMed] [Google Scholar]
  41. Yust I., Smith R. W., Wunderlich J. R., Mann D. L. Temporary inhibition of antibody-dependent, cell-mediated cytotoxicity by pretreatment of human attacking cells with ammonium chloride. J Immunol. 1976 Apr;116(4):1170–1172. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES