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. 1988 Mar 25;16(6):2509–2521. doi: 10.1093/nar/16.6.2509

Transcriptional activity and factor binding are stimulated by separate and distinct sequences in the 5' flanking region of a mouse tRNAAsp gene.

R J Rooney 1, J D Harding 1
PMCID: PMC336386  PMID: 3362674

Abstract

The transcriptional properties of two cloned mouse tRNAAsp genes were examined in vitro. The tRNA(2Asp) gene displays a five fold greater transcriptional activity than the tRNA(1Asp) gene and a greater ability to form stable complexes with transcription factors. Transcription of a hybrid gene with swapped 5' flanking sequences and of 5' flanking region deletion mutants demonstrates that the differential transcription of the genes results from stimulatory sequences in the 5' flanking region of the tRNA(2Asp) gene. Distal sequences including those between positions -53 and -31 stimulate transcription but do not affect factor binding. Proximal sequences between positions -9 and -1 enhance factor binding. Thus, binding of transcription factors and later steps required for transcription can be modulated by separate and distinct 5' flanking sequence motifs in eukaryotic tRNA genes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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