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. 1988 May 11;16(9):3739–3749. doi: 10.1093/nar/16.9.3739

Transcriptional terminators in the caa-cal operon and cai gene.

R Lloubès 1, D Baty 1, C Lazdunski 1
PMCID: PMC336553  PMID: 2453841

Abstract

We have analysed by S1 nuclease mapping the in vivo termination sites of transcription of the caa-cal operon and cai gene. The termination region for caa mRNA (T1A terminator) features characteristics of a rho-independent terminator. This terminator is a convergent transcription terminator, its complementary secondary structure being present at the 3'-end of cai mRNA. The caa-cal mRNA terminator (T2A terminator) has a stable potential secondary structure and shows homology with rho-dependent terminators. In vitro transcription of caa-cal operon demonstrated that the two terminators T1A and T2A are efficient. The 3'-ends of the mRNAs which end at T1A and T2A were analysed by S1 mapping with total RNA purified from a mutant strain deficient in exoribonuclease activities, in particular RNase II. The results suggest that the potential secondary structures of T1A and T2A are sufficiently stable to prevent 3'-end degradation by RNase II. On the other hand, the T2A terminator should be efficient enough to stop transcription through the downstream DNA region involved in pColA replication.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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