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. 1988 May 25;16(10):4389–4406. doi: 10.1093/nar/16.10.4389

A DNA sequence outside the pUB110 minimal replicon is required for normal replication in Bacillus subtilis.

J F Viret 1, J C Alonso 1
PMCID: PMC336637  PMID: 2837734

Abstract

The origin of lagging strand synthesis in pUB110, oriL, has been localized within 140 bases outside the pUB110 minimal replicon. The oriL DNA sequence is a cis-acting and orientation dependent determinant required for normal plasmid replication. Rearrangements affecting oriL cause plasmid instability, lead to the accumulation of replication intermediates and result in a marked reduction of the plasmid copy number in some recombination deficient mutant strains. In addition, deletion of oriL triggers a dnaB-dependent mode of replication. Insertion of the functionally asymmetric oriL region in the proper orientation into pC194 reduces the accumulation of single-stranded DNA during the replication of this plasmid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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