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. 1988 Jul 11;16(13):5973–5989. doi: 10.1093/nar/16.13.5973

Binding of integration host factor (IHF) to the ilvGp1 promoter of the ilvGMEDA operon of Escherichia coli K12.

R F Pereira 1, M J Ortuno 1, R P Lawther 1
PMCID: PMC336841  PMID: 2840636

Abstract

Crude extracts of Escherichia coli K-12 were found to bind DNA restriction fragments containing ilvGp1. Our analysis using a series of restriction fragments and a BamHI linker mutation indicate that a factor binds to ilvGp1 or adjacent to it. Analysis with mutant strains of E. coli K-12 and purified IHF indicate that IHF binds to ilvGp1. Furthermore, both analysis in vivo and in vitro indicate that IHF precludes transcription from ilvGp1.

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Selected References

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