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. Author manuscript; available in PMC: 2012 Sep 28.
Published in final edited form as: J Am Chem Soc. 2011 Sep 2;133(38):14940–14943. doi: 10.1021/ja206743v

Concise Total Synthesis and Stereochemical Revision of (+)-Naseseazines A and B. Regioselective Arylative Dimerization of Diketopiperazine Alkaloids

Justin Kim 1, Mohammad Movassaghi 1,*
PMCID: PMC3369503  NIHMSID: NIHMS323492  PMID: 21875056

Abstract

The concise and enantioselective total syntheses of (+)-naseseazines A and B are described. Our regioselective and directed dimerization of diketopiperazines provides their critical C3–Csp2 linkages, an assembly with plausible biogenetic relevance. We revise the absolute stereochemistry of (+)-naseseazines A and B.


Dimeric diketopiperazine alkaloids, which comprise an important class of natural products, possess an expansive repertoire of biological activities intimately mirroring their structural diversity.1 An important source of diversity of great strategic relevance to their synthesis is the nature of the dimeric linkage. In recent years, much progress has been made toward the rapid construction of the C3sp3–C3'sp32,3,4 as well as the C3sp3–N1'5 bond connectivity. In this report, we aim to broaden the spectrum of dimerization modes that are predisposed for rapid disconnection. We describe herein a Friedel–Crafts based method for the regioselective and directed C3-functionalization of advanced diketopiperazine intermediates employing a range of π-nucleophiles. The concision and broad relevance of this approach to the synthesis of C3-arylated hexahydropyrroloindole alkaloids6 (Figure 1) are highlighted through the first total syntheses of Fijan actinomycete Streptomyces sp. derived alkaloids (+)-naseseazines A (1) and B (2),7 an endeavor culminating in their stereochemical revision.

Figure 1.

Figure 1

Representative C3-arylated hexahydropyrroloindole alkaloids. Revised structures of (+)-naseseazines A and B (12).

In deference to our desire for a maximally convergent synthesis of (+)-naseseazines A (1) and B (2), and consistent with a plausible hypothesis for their biogenesis,8,9 our retrosynthetic analysis commenced with the disconnection of the C3–C7'10 bonds of (+)-1 and (+)-2, affording two diketopiperazine fragments of comparable complexity (Scheme 1). Tetracyclic bromide 6 could be derived from the bromocyclization of diketopiperazine 8,3b whereas pinacolboronate 5 could be prepared from the corresponding bromodiketopiperazine 7. We envisioned that ionization of tetracyclic bromide 6 would provide the necessary C3-electrophile,11,12 while conversion of the pinacolboronate function of diketopiperazine 5 to the corresponding trifluoroborate13 would offer the necessary C7'-nucleophilic partner for a directed and regioselective sp3−sp2 bond formation (Scheme 1).

Scheme 1.

Scheme 1

Retrosynthetic analysis of (+)-naseseazines A and B (12).

In our first generation approach, alanine and proline diketopiperazines (−)-9 and (−)-10, the requisite tetracyclic bromides en route to the corresponding naseseazine alkaloids, were synthesized in 89% and 71% yields, respectively, from readily available Nα-Boc-Nin-Cbz-l-tryptophan methyl ester.14 Exposure of diketopiperazines (−)-9 and (−)-10 to pyridinium tribromide in 2,2,2-trifluoroethanol afforded the respective tetracyclic bromides (+)-11 and (+)-12 in 67% and 51% yields as single diastereomers (Scheme 2). In possession of all the necessary components, we sought to validate the feasibility of our planned Friedel–Crafts-based strategy for C3-arylation at this juncture. Treating a solution of diketopiperazine (−)-10 (1.5 equiv) and tetracyclic bromide (+)-11 (1 equiv) with silver hexafluoroantimonate in nitroethane under optimized conditions (vide infra), afforded a mixture of regioisomeric dimers (−)-13 and (−)-15 in 53% combined yield ((−)-13:(−)-15 = 1:1.4). Similarly, the same proline derived diketopiperazine (−)-10 could be coupled to tetracyclic bromide (+)-12 to give regioisomeric dimers (−)-14 and (−)-16 in 47% combined yield ((−)-14:(−)-16 = 1:1.4). Hydrogenolytic removal of the carboxybenzyl groups on compounds 1316, provided the first access to (+)-iso-naseseazines A (17) and B (18) as well as our target alkaloids (+)-1 and (+)-2, respectively (Scheme 2). Notably, selectivity was observed in favor of the regioisomer with the desired C3–C7' bond connectivity during the coupling reaction.8 While we were pleased with the utility of this new mode of heterodimerization, the low level of regioselectivity in this unguided union prompted further refinement of our new strategy toward a more selective and directed assembly of complex diketopiperazine fragments.

Scheme 2.

Scheme 2

First generation synthesis of (+)-naseseazines A (1) and B (2).a

a Conditions: (a) PyHBr3, 2,2,2-trifluoroethanol, 23 °C. (b) (−)-10, AgSbF6, EtNO2, 23 °C; for (+)-11, 13:15=1:1.4, 53%; for (+)-12, 14:16=1:1.4, 47%. (c) H2, Pd/C, AcOH, 23 °C.

We anticipated that the regioselective arylation of tetracyclic bromide (+)-11 could be achieved by installation of a directing group at an appropriate position of the π-nucleophile.15,16 Importantly, we wished to increase the ratio of (−)-15 and (−)-16 to (−)-13 and (−)-14, respectively. In the early stages of our optimization studies, we selected thiophene, with its innate preference for reaction at the 2-position (Table 1, entry 1, 19:20 = 6.2:1), as our model system. While 3-cuprate, 3-trimethylstannyl, and 3-zinc chloride thiophene derivatives were ineffective as directed nucleophiles in the C3-arylation of bromide (+)-11, the use of the corresponding potassium trifluoroborate derivative proved promising.15,17 We expected that using 18-crown-6 as an additive would confer multiple benefits: increased solubility of the potassium trifluoroborate salt, enhanced nucleophilicity of the arene via ion pairing with the electrophile, and facilitated trifluoroborate elimination by further dissociation of the potassium-aryltrifluoroborate ion pair.18 Indeed, when silver hexafluoroantimonate was introduced to a solution of tetracyclic bromide (+)-11, potassium 3-thiophenetrifluoroborate, and 18-crown-6 in dichloromethane at 23 °C, we obtained the desired C3-arylated product as a mixture of regioisomers favoring the desired C3-adduct (19:20 = 1:1.3). Sub-ambient temperatures were then examined to enhance the regioselectivity for C3; however, selectivity did not improve noticeably at 0 °C in dichloromethane, and conversion to the product was not observed at lower temperatures. Thus, we turned to higher dielectric-constant media to facilitate the ionization event.

Table 1.

Lewis acid-promoted C3-nucleophile substitution.

graphic file with name nihms323492t1.jpg

Entry Nucleophile Solvent, Temp (°C) graphic file with name nihms323492t2.jpg Yield (%)a
1 graphic file with name nihms323492t3.jpg CH2Cl2, 23 graphic file with name nihms323492t4.jpg 77 (6.3:1)b
2 graphic file with name nihms323492t5.jpg EtNO2, −45c graphic file with name nihms323492t6.jpg 50 (17:1)b
3 graphic file with name nihms323492t7.jpg CH2Cl2, 23 graphic file with name nihms323492t8.jpg 60
4 graphic file with name nihms323492t9.jpg CH2Cl2, 23 graphic file with name nihms323492t10.jpg 52
5 graphic file with name nihms323492t11.jpg CH2Cl2, 23 graphic file with name nihms323492t12.jpg 91
6 graphic file with name nihms323492t13.jpg CH2Cl2, 23 graphic file with name nihms323492t14.jpg 89
7 graphic file with name nihms323492t15.jpg CH2Cl2, 23 graphic file with name nihms323492t16.jpg 50
8 graphic file with name nihms323492t17.jpg EtNO2, −45c graphic file with name nihms323492t18.jpg 26 (3.9:1)d
9 graphic file with name nihms323492t19.jpg EtNO2, −45c graphic file with name nihms323492t20.jpg 54 (2.4:1)d
10 graphic file with name nihms323492t21.jpg EtNO2, 23c graphic file with name nihms323492t22.jpg 59
a

Isolated yield; average of two experiments; AgSbF6 (2.0 equiv) and nucleophile (2.0 equiv).

b

Regioisomeric ratios determined by 1H NMR. Major regioisomer shown.

c

18-crown-6 (2.0 equiv).

d

The minor regioisomer is the para-adduct.

Nitrile-based solvents such as acetonitrile and benzonitrile enabled ionization; however, tight solvent-carbocation interactions significantly impeded the nucleophilic reaction, and hydroxylation19 and Ritter reaction adducts20 formed in significant amounts as principal byproducts. Similarly, use of N,N'-dimethylformamide resulted exclusively in hydroxyl and formate addition products.21 Ultimately, we discovered that the use of nitroalkane solvents was most effective for the desired transformation. The level of regioselection improved for the C3-thiophenyl adduct at −25 °C in nitromethane (19:20 = 1:2.8). Under optimal conditions, treatment of (+)-11 with 3-thiophenetrifluoroborate at −45 °C in nitroethane provided the desired product with an excellent level of regioselection (Table 1, entry 2, 50%, 19:20 = 1:17).9

Encouraged by the efficiency and high regioselectivity observed in the trifluoroborate-directed thiophenylation of bromide (+)-11, we sought to examine the generality of this chemistry with respect to other π-nucleophiles. Table 1 demonstrates the substrate scope for the nucleophilic addition into the C3-position of the tetracyclic bromide (+)-11: allyltri-n-butylstannane (Table 1, entry 3, 60%), allyltrimethylsilane (Table 1, entry 4, 52%), as well as (isopropenyloxy) trimethylsilane (Table 1, entry 5, 91%) act as suitable nucleophiles. Notably, vicinal quaternary stereocenters can also be installed efficiently, as demonstrated by the addition of methyl trimethysilyl dimethylketene acetal (Table 1, entry 6, 89%). Electron rich arenes, such as thiophene, (Table 1, entry 1, 76%) and electron neutral arenes, best represented by toluene (Table 1, entry 7, 50%), are also able to incorporate efficiently. Toluene itself adds with exclusive selectivity for the para-position, a preference that can be reversed using the trifluoroborate group.

The regioselectivity of aryltrifluoroborate addition, although moderate, is eroded in the presence of ortho-substituents such as in ortho-tolyltrifluoroborate (Table 1, entry 8, 25:24 = 3.5:1) and 2-methoxyphenyl trifluoroborate (Table 1, entry 9, 26:26' = 2.7:1), likely reflecting a sterically demanding transition state in the formation of quaternary stereocenters. Unfortunately, electron deficient arenes, such as potassium 4-acetylphenyl-trifluoroborate, lead to exclusive C3-fluorination via fluoride transfer from the aryltrifluoroborate. Nucleophiles without a competent electrofuge, such as styrene, result in polymerization. This problem can be circumvented through use of a trifluoroborate group, which likely eliminates faster than a proton (Table 1, entry 10, 59%). In general, the efficiency of the reactions appears to correlate well with the relative nucleophilicity of the compounds.22 The mildness of the reaction conditions is also of particular note. The utility of the method in late-stage applications is highlighted by the preservation of stereochemical integrity at C11 and C15, epimerizable stereocenters3b,5e consequential for the further derivatization of advanced intermediates.3c

Our planned strategy for the directed union of advanced stage diketopiperazine fragments for the synthesis of (+)-naseseazines A (1) and B (2) required the synthesis of the trifluoroborate (−)-33 (Scheme 3). Starting from commercially available 6-bromo-3-carboxaldehyde (28), benzyloxycarbonylation followed by a Horner–Wadsworth–Emmons reaction with Boc-α-phosphonoglycine trimethylester afforded enamide 29 in 97% yield (2-steps).23 Catalytic asymmetric hydrogenation of the olefin using (S,S)-Et-DUPHOS-Rh (1.8 mol%) efficiently provided the bromotryptophan (+)-30 in 97% yield and >99% ee.24 An expeditious two-step sequence afforded the bromocyclodipeptide (−)-31 in 75% yield from bromotryptophan derivative (+)-30 without a need for chromatography. Our initial efforts to introduce the pinacol boronic ester function via palladium-catalyzed cross-coupling with pinacolborane25 were mired by undesired carboxybenzyl deprotection as well as the formation of significant amounts of a C7'-reduction byproduct. After significant experimentation, we recognized that employing Buchwald’s aminobiphenyl-(XPhos)PdCl precatalyst (34, 5 mol%),26 XPhos (15 mol%), K3PO4 (3 equiv), and bis(pinacolato)diboron (3 equiv) in DMSO at 60 °C effectively afford the desired pinacol boronate (−)-32 in 65% yield (Scheme 3). Treatment of the pinacol boronate (−)-32 with aqueous potassium hydrogen difluoride gave the key potassium trifluoroborate (−)-33 in 88% yield.13

Scheme 3.

Scheme 3

Concise and directed synthesis of (+)-naseseazines A (1) and B (2).a

a Conditions: (a) CbzCl, Et3N, DMAP, CH2Cl2, 100%. (b) Boc-α-phosphonoglycine trimethylester, DBU, CH2Cl2, 97%. (c) H2 (80 psi), (S,S)-Et-DUPHOS-Rh (1.8 mol%), CH2Cl2, MeOH, 97%, >99% ee. (d) TFA, CH2Cl2; EDC•HCl, HOBt, Et3N, Boc-l-Pro. (e) TFA, CH2Cl2; NH4OH, MeOH, 75% (2-steps). (f) 2-aminobiphenyl(XPhos)PdCl (5 mol%), XPhos (15 mol%), (BPin)2, K3PO4, DMSO, 60 °C, 65%. (g) KHF2 (aq.), MeOH, 88%. (h) AgSbF6, 18-crown-6, EtNO2, 23 °C. (i) H2, Pd/C, AcOH, 23 °C.

Treatment of alanine-derived tetracyclic bromide (+)-11 (1 equiv) with tetracyclic potassium trifluoroborate (−)-33 (1.5 equiv) in the presence of silver hexafluoroantimonate and 18-crown-6 in nitroethane at 23 °C afforded the desired (−)-dicarboxybenzyl naseseazine A (15) in 56% yield with complete regioselection (Scheme 3). Under identical conditions, exposure of proline derived tetracyclic bromide (+)-12 (1 equiv) with tetracyclic potassium trifluoroborate (−)-33 (1.5 equiv) gave (−)-dicarboxybenzyl naseseazine B (16) in 50% yield as a single regioisomer (Scheme 3). Excitingly, the ability to override the innate nucleophilic tendencies of the indole substructure through an appropriately positioned directing group has broad implications toward the synthesis of related congeners of different regioisomeric constitutions about the C3sp3–Csp2 dimeric linkage, such as in (+)-asperazine,27 (+)-pestalazine,28 as well as the entire superfamily of oligomeric cyclotryptamines,1 represented by (+)-caledonine (Figure 1).29

Hydrogenolytic removal of the benzyloxycarbonyl groups from (−)-15 and (−)-16 with palladium on carbon in acetic acid provided (+)-naseseazine A (1) {[α]22D = +123 (c 0.12, CH3OH); lit. [α]23D = +139 (c 0.10, CH3OH)}7 and (+)-naseseazine B (2) {[α]22D = +101 (c 0.23, CH3OH); lit. [α]23D = +95 (c 0.08, CH3OH)},7 respectively, each in 80% yield (Scheme 3). All of the spectroscopic data for (+)-1 and (+)-2 matched those reported in the literature.7 The agreement between the optical rotation sign of our synthetic samples of naseseazine A (+)-1 and B (+)-2 and those of the natural alkaloids stands in direct contrast to the predicted absolute stereochemistry of (+)-1 and (+)-2 based on C3 Marfey’s analysis30 of degradation products. Given the use of l-amino acid derivatives in our synthesis of (+)-naseseazine A (1) and B (2), we revise the absolute stereochemistry of these dimeric natural alkaloids (Figure 1).

We have developed a general approach to dimeric diketopiperazine alkaloids containing a C3sp3–Csp2 linkage, with the solution yielding a concise 9-step total synthesis of (+)-naseseazines A (1) and B (2). Our mild and highly regioselective Friedel–Crafts based coupling strategy, featuring the formation of quaternary stereogenic centers, facilitated the late-stage union of advanced diketopiperazine structures in a convergent manner. Our total syntheses of (+)-1 and (+)-2 have also enabled the revision of the absolute stereochemistry of these natural products. This new fragment assembly strategy, ideated from a close adherence to biogenetically relevant intermediates and transformations, highlights the power of a strategic framework guided by retrobiosynthetic31 analysis.

Supplementary Material

1_si_001

Acknowledgement

We acknowledge financial support by NIH-NIGMS (GM089732), Amgen, and DuPont. M.M. is a Camille Dreyfus Teacher-Scholar. J.K. acknowledges a National Defense Science and Engineering Graduate Fellowship. We thank Dr. Omar K. Ahmad and Dr. Nicolas Boyer for helpful discussions. We are grateful to Prof. R. J. Capon for a communication regarding the discrepancy between our respective stereochemical assignments of (+)-naseseazines A and B.

Footnotes

Supporting Information. Experimental procedures, spectroscopic data, and copies of UV-vis, 1H, 13C, and 19F NMR spectra. This material is free of charge via the Internet at http://pubs.acs.org.

References

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