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. 1988 Mar 11;16(5 Pt A):1715–1728. doi: 10.1093/nar/16.5.1715

A backtranslation method based on codon usage strategy.

G Pesole 1, M Attimonelli 1, S Liuni 1
PMCID: PMC338166  PMID: 3281142

Abstract

This study describes a method for the backtranslation of an aminoacidic sequence, an extremely useful tool for various experimental approaches. It involves two computer programs CLUSTER and BACKTR written in Fortran 77 running on a VAX/VMS computer. CLUSTER generates a reliable codon usage table through a cluster analysis, based on a chi 2-like distance between the sequences. BACKTR produces backtranslated sequences according to different options when use is made of the codon usage table obtained in addition to selecting the least ambiguous potential oligonucleotide probes within an aminoacidic sequence. The method was tested by applying it to 158 yeast genes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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