Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1988 Mar 25;16(5 Pt B):2111–2126. doi: 10.1093/nar/16.5.2111

Close physical linkage of the murine Ren-1 and Ren-2 loci.

K J Abel 1, K W Gross 1
PMCID: PMC338203  PMID: 2833727

Abstract

In addition to the Ren-1 gene common to all mice, some inbred strains carry a second copy of the renin structural gene, Ren-2. These two loci are tightly linked genetically on mouse chromosome one. We have used pulsed field gel electrophoresis (PFGE) to study the physical arrangement of the two renin genes in the inbred strain DBA/2. PFGE mapping permitted the construction of a restriction map of the Ren loci spanning roughly 120 Kb. The results indicate that the genes are transcribed in the same relative direction, that Ren-2 lies upstream relative to Ren-1, and that the respective coding sequences are separated by approximately 20 Kb.

Full text

PDF
2113

Images in this article

2116Image
on p.2116
2117Image
on p.2117
2118Image
on p.2118
2119Image
on p.2119
2121Image
on p.2121
2122Image
on p.2122

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barlow D. P., Bućan M., Lehrach H., Hogan B. L., Gough N. M. Close genetic and physical linkage between the murine haemopoietic growth factor genes GM-CSF and Multi-CSF (IL3). EMBO J. 1987 Mar;6(3):617–623. doi: 10.1002/j.1460-2075.1987.tb04799.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carle G. F., Frank M., Olson M. V. Electrophoretic separations of large DNA molecules by periodic inversion of the electric field. Science. 1986 Apr 4;232(4746):65–68. doi: 10.1126/science.3952500. [DOI] [PubMed] [Google Scholar]
  3. Chirgwin J. M., Schaefer I. M., Rotwein P. S., Piccini N., Gross K. W., Naylor S. L. Human renin gene is on chromosome 1. Somat Cell Mol Genet. 1984 Jul;10(4):415–421. doi: 10.1007/BF01535637. [DOI] [PubMed] [Google Scholar]
  4. Dickinson D. P., Gross K. W., Piccini N., Wilson C. M. Evolution and variation of renin genes in mice. Genetics. 1984 Nov;108(3):651–667. doi: 10.1093/genetics/108.3.651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  6. Field L. J., Gross K. W. Ren-1 and Ren-2 loci are expressed in mouse kidney. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6196–6200. doi: 10.1073/pnas.82.18.6196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Field L. J., Philbrick W. M., Howles P. N., Dickinson D. P., McGowan R. A., Gross K. W. Expression of tissue-specific Ren-1 and Ren-2 genes of mice: comparative analysis of 5'-proximal flanking regions. Mol Cell Biol. 1984 Nov;4(11):2321–2331. doi: 10.1128/mcb.4.11.2321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gardiner K., Laas W., Patterson D. Fractionation of large mammalian DNA restriction fragments using vertical pulsed-field gradient gel electrophoresis. Somat Cell Mol Genet. 1986 Mar;12(2):185–195. doi: 10.1007/BF01560665. [DOI] [PubMed] [Google Scholar]
  9. Hagenbüchle O., Bovey R., Young R. A. Tissue-specific expression of mouse-alpha-amylase genes: nucleotide sequence of isoenzyme mRNAs from pancreas and salivary gland. Cell. 1980 Aug;21(1):179–187. doi: 10.1016/0092-8674(80)90125-7. [DOI] [PubMed] [Google Scholar]
  10. Hardy D. A., Bell J. I., Long E. O., Lindsten T., McDevitt H. O. Mapping of the class II region of the human major histocompatibility complex by pulsed-field gel electrophoresis. Nature. 1986 Oct 2;323(6087):453–455. doi: 10.1038/323453a0. [DOI] [PubMed] [Google Scholar]
  11. Herrmann B. G., Barlow D. P., Lehrach H. A large inverted duplication allows homologous recombination between chromosomes heterozygous for the proximal t complex inversion. Cell. 1987 Mar 13;48(5):813–825. doi: 10.1016/0092-8674(87)90078-x. [DOI] [PubMed] [Google Scholar]
  12. Holm I., Ollo R., Panthier J. J., Rougeon F. Evolution of aspartyl proteases by gene duplication: the mouse renin gene is organized in two homologous clusters of four exons. EMBO J. 1984 Mar;3(3):557–562. doi: 10.1002/j.1460-2075.1984.tb01846.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jagodzinski L. L., Sargent T. D., Yang M., Glackin C., Bonner J. Sequence homology between RNAs encoding rat alpha-fetoprotein and rat serum albumin. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3521–3525. doi: 10.1073/pnas.78.6.3521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lawrance S. K., Smith C. L., Srivastava R., Cantor C. R., Weissman S. M. Megabase-scale mapping of the HLA gene complex by pulsed field gel electrophoresis. Science. 1987 Mar 13;235(4794):1387–1390. doi: 10.1126/science.3029868. [DOI] [PubMed] [Google Scholar]
  15. Maniatis T., Fritsch E. F., Lauer J., Lawn R. M. The molecular genetics of human hemoglobins. Annu Rev Genet. 1980;14:145–178. doi: 10.1146/annurev.ge.14.120180.001045. [DOI] [PubMed] [Google Scholar]
  16. Morris B. J., de Zwart R. T., Young J. A. Renin in mouse but not in rat submandibular glands. Experientia. 1980 Nov 15;36(11):1333–1334. doi: 10.1007/BF01969621. [DOI] [PubMed] [Google Scholar]
  17. Mullins J. J., Burt D. W., Windass J. D., McTurk P., George H., Brammar W. J. Molecular cloning of two distinct renin genes from the DBA/2 mouse. EMBO J. 1982;1(11):1461–1466. doi: 10.1002/j.1460-2075.1982.tb01338.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Niall H. D., Hogan M. L., Sauer R., Rosenblum I. Y., Greenwood F. C. Sequences of pituitary and placental lactogenic and growth hormones: evolution from a primordial peptide by gene reduplication. Proc Natl Acad Sci U S A. 1971 Apr;68(4):866–870. doi: 10.1073/pnas.68.4.866. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Panthier J. J., Dreyfus M., Roux T. L., Rougeon F. Mouse kidney and submaxillary gland renin genes differ in their 5' putative regulatory sequences. Proc Natl Acad Sci U S A. 1984 Sep;81(17):5489–5493. doi: 10.1073/pnas.81.17.5489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Panthier J. J., Foote S., Chambraud B., Strosberg A. D., Corvol P., Rougeon F. Complete amino acid sequence and maturation of the mouse submaxillary gland renin precursor. Nature. 1982 Jul 1;298(5869):90–92. doi: 10.1038/298090a0. [DOI] [PubMed] [Google Scholar]
  21. Panthier J. J., Holm I., Rougeon F. The mouse Rn locus: S allele of the renin regulator gene results from a single structural gene duplication. EMBO J. 1982;1(11):1417–1421. doi: 10.1002/j.1460-2075.1982.tb01332.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Pellicer A., Wigler M., Axel R., Silverstein S. The transfer and stable integration of the HSV thymidine kinase gene into mouse cells. Cell. 1978 May;14(1):133–141. doi: 10.1016/0092-8674(78)90308-2. [DOI] [PubMed] [Google Scholar]
  23. Piccini N., Knopf J. L., Gross K. W. A DNA polymorphism, consistent with gene duplication, correlates with high renin levels in the mouse submaxillary gland. Cell. 1982 Aug;30(1):205–213. doi: 10.1016/0092-8674(82)90026-5. [DOI] [PubMed] [Google Scholar]
  24. Russell G. J., Walker P. M., Elton R. A., Subak-Sharpe J. H. Doublet frequency analysis of fractionated vertebrate nuclear DNA. J Mol Biol. 1976 Nov;108(1):1–23. doi: 10.1016/s0022-2836(76)80090-3. [DOI] [PubMed] [Google Scholar]
  25. SWARTZ M. N., TRAUTNER T. A., KORNBERG A. Enzymatic synthesis of deoxyribonucleic acid. XI. Further studies on nearest neighbor base sequences in deoxyribonucleic acids. J Biol Chem. 1962 Jun;237:1961–1967. [PubMed] [Google Scholar]
  26. Schwartz D. C., Cantor C. R. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984 May;37(1):67–75. doi: 10.1016/0092-8674(84)90301-5. [DOI] [PubMed] [Google Scholar]
  27. Soubrier F., Panthier J. J., Corvol P., Rougeon F. Molecular cloning and nucleotide sequence of a human renin cDNA fragment. Nucleic Acids Res. 1983 Oct 25;11(20):7181–7190. doi: 10.1093/nar/11.20.7181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  29. Wilson J. X. The renin-angiotensin system in nonmammalian vertebrates. Endocr Rev. 1984 Winter;5(1):45–61. doi: 10.1210/edrv-5-1-45. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES