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. 1988 Mar 25;16(5 Pt B):2247–2262. doi: 10.1093/nar/16.5.2247

The adenovirus tripartite leader sequence can alter nuclear and cytoplasmic metabolism of a non-adenovirus mRNA within infected cells.

M A Moore 1, T Shenk 1
PMCID: PMC338213  PMID: 3357776

Abstract

All mRNAs encoded by the adenovirus major late transcription unit share a common 5' noncoding region, 200 nucleotides in length, termed the tripartite leader sequence. To assess function of the tripartite leader, recombinant viruses were prepared which carried either a bona fide herpes simplex virus thymidine kinase gene or a modified thymidine kinase gene whose normal 5' noncoding domain was replaced with the adenovirus leader sequence. The tripartite leader simultaneously decreased the nuclear half-life and increased the cytoplasmic half-life of the thymidine kinase-specific mRNA. The tripartite leader stabilized the non-adenovirus mRNA only within the environment of an adenovirus-infected cell during the late phase of the infectious cycle.

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Selected References

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